GPR137C (NM_001099652) Human Tagged ORF Clone

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SKU
RC218990
GPR137C (Myc-DDK-tagged)-Human G protein-coupled receptor 137C (GPR137C)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GPR137C
Synonyms TM7SF1L2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC218990 representing NM_001099652
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGGTGTCCGTGCCGGGTCCGGCGGCCGCTGCCGCCCCCGCAGCCGGCCGCGAGCCCTCCACGCCCG
GCGGGGGCAGCGGAGGCGGAGGCGCCGTCGCTGCAGCCTCAGGCGCCGCGGTGCCGGGCTCCGTGCAGTT
GGCGCTGAGCGTCCTGCACGCCCTGCTCTACGCCGCGCTGTTCGCCTTTGCCTACCTGCAGCTGTGGCGG
CTGCTCCTGTACCGCGAGCGGCGGCTGAGTTACCAGAGCCTCTGCCTCTTCCTCTGTCTCCTGTGGGCAG
CGCTCAGGACCACCCTCTTCTCCGCCGCCTTCTCGCTCAGCGGCTCCCTGCCCTTGCTCCGGCCGCCCGC
TCACCTGCACTTCTTCCCCCACTGGCTGCTCTACTGCTTCCCCTCCTGTCTCCAGTTCTCCACGCTCTGT
CTCCTCAACCTCTACCTGGCGGAGGTTATATGTAAAGTCAGATGTGCCACTGAACTTGACAGACACAAAA
TTCTACTGCATTTGGGCTTTATAATGGCAAGCCTGCTCTTTTTAGTGGTGAACTTGACTTGCGCAATGCT
AGTTCATGGAGATGTCCCAGAAAATCAGTTGAAGTGGACTGTGTTTGTTCGAGCATTAATTAATGATAGC
CTGTTTATTCTTTGTGCCATCTCTTTAGTGTGTTACATATGCAAAATTACAAAAATGTCATCAGCTAATG
TCTACCTCGAATCAAAGGGTATGTCTCTGTGCCAGACTGTCGTCGTGGGCTCTGTAGTCATTCTTCTGTA
CTCTTCCAGAGCTTGTTATAATTTGGTGGTGGTCACCATATCTCAGGATACATTAGAAAGTCCATTTAAT
TATGGCTGGGATAATCTTTCAGATAAGGCTCATGTAGAAGACATAAGTGGAGAAGAGTATATAGTATTTG
GAATGGTCCTCTTTCTGTGGGAACATGTGCCAGCATGGTCGGTGGTACTGTTTTTCCGGGCACAGAGATT
AAACCAGAATTTGGCACCTGCTGGCATGATAAATAGTCACAGTTATAGTTCCAGAGCTTACTTTTTCGAC
AATCCAAGACGATATGATAGTGATGATGACCTGCCAAGACTGGGAAGTTCAAGAGAAGGAAGTTTACCAA
ATTCGCAAAGTTTGGGCTGGTATGGCACCATGACTGGGTGTGGCAGCAGCAGTTACACAGTCACTCCCCA
CCTGAATGGACCTATGACAGATACTGCTCCTTTGCTCTTTACTTGTAGTAATTTAGATTTGAACAATCAT
CATAGCTTATATGTGACACCACAAAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC218990 representing NM_001099652
Red=Cloning site Green=Tags(s)

MRVSVPGPAAAAAPAAGREPSTPGGGSGGGGAVAAASGAAVPGSVQLALSVLHALLYAALFAFAYLQLWR
LLLYRERRLSYQSLCLFLCLLWAALRTTLFSAAFSLSGSLPLLRPPAHLHFFPHWLLYCFPSCLQFSTLC
LLNLYLAEVICKVRCATELDRHKILLHLGFIMASLLFLVVNLTCAMLVHGDVPENQLKWTVFVRALINDS
LFILCAISLVCYICKITKMSSANVYLESKGMSLCQTVVVGSVVILLYSSRACYNLVVVTISQDTLESPFN
YGWDNLSDKAHVEDISGEEYIVFGMVLFLWEHVPAWSVVLFFRAQRLNQNLAPAGMINSHSYSSRAYFFD
NPRRYDSDDDLPRLGSSREGSLPNSQSLGWYGTMTGCGSSSYTVTPHLNGPMTDTAPLLFTCSNLDLNNH
HSLYVTPQN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001099652
ORF Size 1287 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001099652.2
RefSeq Size 3908 bp
RefSeq ORF 1290 bp
Locus ID 283554
UniProt ID Q8N3F9, B3KW22, Q6AWC7
Cytogenetics 14q22.1
Protein Families Druggable Genome, Transmembrane
MW 46.9 kDa
Summary Lysosomal integral membrane protein that may regulate MTORC1 complex translocation to lysosomes.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001099652" in other vectors (4)
SKU Description Size Price
RC218990L3 Lenti ORF clone of Human G protein-coupled receptor 137C (GPR137C), Myc-DDK-tagged 10 ug
$757.00
RC218990L4 Lenti ORF clone of Human G protein-coupled receptor 137C (GPR137C), mGFP tagged 10 ug
$757.00
RG218990 GPR137C (tGFP-tagged) - Human G protein-coupled receptor 137C (GPR137C) 10 ug
$657.00
SC316751 GPR137C (untagged)-Human G protein-coupled receptor 137C (GPR137C) 10 ug
$457.00

Plasmid Map for RC218990

Download sequence for

Cloning scheme for RC218990

Circular map for RC218990

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