C16orf89 (NM_152459) Human Tagged ORF Clone

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SKU
RC218930
C16orf89 (Myc-DDK-tagged)-Human chromosome 16 open reading frame 89 (C16orf89), transcript variant 1
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol C16orf89
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC218930 representing NM_152459
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAGCCTGGGGCTGCTGCTCCTGCTCTTACTGACAGCACTGCCACCGCTGTGGTCCTCCTCACTGC
CTGGGCTGGACACTGCTGAAAGTAAAGCCACCATTGCAGACCTGATCCTGTCTGCGCTGGAGAGAGCCAC
CGTCTTCCTAGAACAGAGGCTGCCTGAAATCAACCTGGATGGCATGGTGGGGGTCCGAGTGCTGGAAGAG
CAGCTAAAAAGTGTCCGGGAGAAGTGGGCCCAGGAGCCCCTGCTGCAGCCGCTGAGCCTGCGCGTGGGGA
TGCTGGGGGAGAAGCTGGAGGCTGCCATCCAGAGATCCCTCCACTACCTCAAGCTGAGTGATCCCAAGTA
CCTAAGAGAGTTCCAGCTGACCCTCCAGCCCGGGTTTTGGAAGCTCCCACATGCCTGGATCCACACTGAT
GCCTCCTTGGTGTACCCCACGTTCGGGCCCCAGGACTCATTCTCAGAGGAGAGAAGTGACGTGTGCCTGG
TGCAGCTGCTGGGAACCGGGACGGACAGCAGCGAGCCCTGCGGCCTCTCAGACCTCTGCAGGAGCCTCAT
GACCAAGCCCGGCTGCTCAGGCTACTGCCTGTCCCACCAACTGCTCTTCTTCCTCTGGGCCAGAATGAGG
GGGTGCACACAGGGACCACTCCAACAGAGCCAGGACTATATCAACCTCTTCTGCGCCAACATGATGGACT
TGAACCGCAGAGCTGAGGCCATCGGATACGCCTACCCTACCCGGGACATCTTCATGGAAAACATCATGTT
CTGTGGAATGGGCGGCTTCTCCGACTTCTACAAGCTCCGGTGGCTGGAGGCCATTCTCAGCTGGCAGAAA
CAGCAGGAAGGATGCTTCGGGGAGCCTGATGCTGAAGATGAAGAATTATCTAAAGCTATTCAATATCAGC
AGCATTTTTCGAGGAGAGTGAAGAGGCGAGAAAAACAATTTCCAGATTCTCGCTCTGTTGCTCAGGCTGG
AGTACAGTGGCGCAATCTCGGCTCACTGCAACCTTTGCCTCCTGGGTTCAAGCAATTCTCTTGCCTCATC
CTCCCGAGTAGCTGGGACTACAGGAGCGTGCCACCATACCTGGCTAATTTTTATATTTTTTTAGTAGAGA
CAGGGTTTCATCATGTTGCTCATGCTGGTCTCGAACTCCTGATCTCAAGAGATCCGCCCACCTCAGGCTC
CCAAAGTGTGGGATTA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC218930 representing NM_152459
Red=Cloning site Green=Tags(s)

MASLGLLLLLLLTALPPLWSSSLPGLDTAESKATIADLILSALERATVFLEQRLPEINLDGMVGVRVLEE
QLKSVREKWAQEPLLQPLSLRVGMLGEKLEAAIQRSLHYLKLSDPKYLREFQLTLQPGFWKLPHAWIHTD
ASLVYPTFGPQDSFSEERSDVCLVQLLGTGTDSSEPCGLSDLCRSLMTKPGCSGYCLSHQLLFFLWARMR
GCTQGPLQQSQDYINLFCANMMDLNRRAEAIGYAYPTRDIFMENIMFCGMGGFSDFYKLRWLEAILSWQK
QQEGCFGEPDAEDEELSKAIQYQQHFSRRVKRREKQFPDSRSVAQAGVQWRNLGSLQPLPPGFKQFSCLI
LPSSWDYRSVPPYLANFYIFLVETGFHHVAHAGLELLISRDPPTSGSQSVGL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_152459
ORF Size 1206 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152459.4, NP_689672.4
RefSeq Size 1898 bp
RefSeq ORF 1209 bp
Locus ID 146556
UniProt ID Q6UX73
Cytogenetics 16p13.3
MW 49.2 kDa
Summary This gene is expressed predominantly in the thyroid. Based on expression patterns similar to thyroid transcription factors and proteins, this gene may function in the development and function of the thyroid. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Oct 2011
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Documents
Resources
"NM_152459" in other vectors (4)
SKU Description Size Price
RC218930L3 Lenti ORF clone of Human chromosome 16 open reading frame 89 (C16orf89), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC218930L4 Lenti ORF clone of Human chromosome 16 open reading frame 89 (C16orf89), transcript variant 1, mGFP tagged 10 ug
$757.00
RG218930 C16orf89 (tGFP-tagged) - Human chromosome 16 open reading frame 89 (C16orf89), transcript variant 1 10 ug
$657.00
SC317721 C16orf89 (untagged)-Human chromosome 16 open reading frame 89 (C16orf89), transcript variant 1 10 ug
$503.00

Plasmid Map for RC218930

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Cloning scheme for RC218930

Circular map for RC218930

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