HOXB5 (NM_002147) Human Tagged ORF Clone

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SKU
RC218740
HOXB5 (Myc-DDK-tagged)-Human homeobox B5 (HOXB5)
  $450.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol HOXB5
Synonyms HHO.C10; HOX2; Hox2.1; HOX2A; HU-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC218740 representing NM_002147
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTCGTACTTTGTAAACTCCTTCTCGGGGCGTTATCCAAATGGCCCGGACTATCAGTTGCTAAATT
ATGGCAGTGGCAGCTCTCTGAGCGGCTCTTACAGGGATCCCGCTGCCATGCACACCGGCTCTTACGGCTA
CAATTACAATGGGATGGACCTCAGCGTCAACCGCTCCTCGGCCTCCTCCAGCCACTTTGGGGCGGTGGGC
GAGAGCTCGCGCGCCTTCCCCGCGCCCGCCCAGGAGCCCCGCTTCAGGCAAGCGGCTTCGAGCTGCTCCC
TGTCCTCGCCCGAGTCCCTGCCCTGCACCAACGGCGACAGCCACGGCGCCAAGCCCTCTGCTTCGTCCCC
CTCCGACCAGGCGACCTCAGCCAGCTCCAGCGCCAATTTCACCGAAATAGACGAGGCCAGCGCGTCCTCG
GAGCCTGAGGAAGCGGCAAGCCAGCTAAGCAGCCCCAGCCTAGCTCGGGCGCAGCCAGAGCCCATGGCCA
CCTCCACAGCCGCGCCCGAGGGGCAGACTCCGCAGATATTCCCCTGGATGAGGAAGCTTCACATCAGCCA
TGATATGACCGGGCCGGACGGGAAAAGGGCCCGGACCGCGTATACCCGCTACCAGACCCTGGAGCTGGAA
AAGGAGTTCCACTTCAACCGCTACCTGACCCGGCGACGGCGCATCGAGATCGCCCACGCACTCTGCCTGT
CCGAGCGCCAGATCAAGATCTGGTTCCAGAACCGGCGCATGAAGTGGAAGAAGGACAACAAATTGAAAAG
TATGAGCCTGGCTACAGCTGGCAGCGCCTTCCAGCCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC218740 representing NM_002147
Red=Cloning site Green=Tags(s)

MSSYFVNSFSGRYPNGPDYQLLNYGSGSSLSGSYRDPAAMHTGSYGYNYNGMDLSVNRSSASSSHFGAVG
ESSRAFPAPAQEPRFRQAASSCSLSSPESLPCTNGDSHGAKPSASSPSDQATSASSSANFTEIDEASASS
EPEEAASQLSSPSLARAQPEPMATSTAAPEGQTPQIFPWMRKLHISHDMTGPDGKRARTAYTRYQTLELE
KEFHFNRYLTRRRRIEIAHALCLSERQIKIWFQNRRMKWKKDNKLKSMSLATAGSAFQP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_002147
ORF Size 807 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002147.2, NP_002138.1
RefSeq Size 1835 bp
RefSeq ORF 810 bp
Locus ID 3215
UniProt ID P09067
Cytogenetics 17q21.32
Domains homeobox
Protein Families Transcription Factors
MW 29.3 kDa
Summary This gene is a member of the Antp homeobox family and encodes a nuclear protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17. The encoded protein functions as a sequence-specific transcription factor that is involved in lung and gut development. Increased expression of this gene is associated with a distinct biologic subset of acute myeloid leukemia (AML) and the occurrence of bronchopulmonary sequestration (BPS) and congenital cystic adenomatoid malformation (CCAM) tissue. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_002147" in other vectors (6)
SKU Description Size Price
RC218740L1 Lenti ORF clone of Human homeobox B5 (HOXB5), Myc-DDK-tagged 10 ug
$750.00
RC218740L2 Lenti ORF clone of Human homeobox B5 (HOXB5), mGFP tagged 10 ug
$750.00
RC218740L3 Lenti ORF clone of Human homeobox B5 (HOXB5), Myc-DDK-tagged 10 ug
$750.00
RC218740L4 Lenti ORF clone of Human homeobox B5 (HOXB5), mGFP tagged 10 ug
$750.00
RG218740 HOXB5 (tGFP-tagged) - Human homeobox B5 (HOXB5) 10 ug
$650.00
SC118779 HOXB5 (untagged)-Human homeobox B5 (HOXB5) 10 ug
$450.00

Plasmid Map for RC218740

Download sequence for

Cloning scheme for RC218740

Circular map for RC218740

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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