DOT1L (NM_032482) Human Tagged ORF Clone
- TrueORF®
SKU
RC218547
DOT1L (Myc-DDK-tagged)-Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L)
$1,972.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | DOT1L |
| Synonyms | DOT1; KMT4 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_032482 |
| ORF Size | 4611 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_032482.3 |
| RefSeq Size | 4889 bp |
| RefSeq ORF | 4614 bp |
| Locus ID | 84444 |
| UniProt ID | Q8TEK3 |
| Cytogenetics | 19p13.3 |
| Domains | AT_hook |
| Protein Families | Druggable Genome |
| Protein Pathways | Lysine degradation |
| MW | 164.7 kDa |
| Summary | The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. provided by RefSeq, Aug 2011 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_032482" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC218547L1 | Lenti-ORF clone of DOT1L (Myc-DDK-tagged)-Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L) | 10 ug |
$2,272.00
|
|
| RC218547L2 | Lenti-ORF clone of DOT1L (mGFP-tagged)-Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L) | 10 ug |
$2,272.00
|
|
| RC218547L3 | Lenti-ORF clone of DOT1L (Myc-DDK-tagged)-Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L) | 10 ug |
$2,272.00
|
|
| RC218547L4 | Lenti-ORF clone of DOT1L (mGFP-tagged)-Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L) | 10 ug |
$2,272.00
|
|
| RG218547 | DOT1L (tGFP-tagged) - Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L) | 10 ug |
$2,172.00
|
|
| SC315370 | DOT1L (untagged)-Human DOT1-like, histone H3 methyltransferase (S. cerevisiae) (DOT1L) | 10 ug |
$1,315.00
|
|
Citations
Targeting senescence-associated secretory phenotype macrophage: apigenin inhibits DOT1L-dependent H3K79me2 at IL1A to fight SASP in senescent macrophages
Ni, Ma, Xu
Front Mol Biosci (2026) 13, 1801780-1801780 DOI: 10.3389/fmolb.2026.1801780
Ni, Ma, Xu
Front Mol Biosci (2026) 13, 1801780-1801780 DOI: 10.3389/fmolb.2026.1801780
Transcriptomic Profiling Identifies Higher DOT1L Expression as a Candidate Biomarker for a Positive Response to Gemcitabine in Cholangiocarcinoma
DU, Moi, Hung et al
Cancer Genomics Proteomics (2026) 23 (4), 714-729 DOI: 10.21873/cgp.20598
DU, Moi, Hung et al
Cancer Genomics Proteomics (2026) 23 (4), 714-729 DOI: 10.21873/cgp.20598
AI Summary: OriGene’s human DOT1L overexpression plasmid (RC218547) was used to generate CCA cell lines with elevated DOT1L expression, enabling functional studies that demonstrated DOT1L’s role in enhancing gemcitabine sensitivity in cholangiocarcinoma.
Snippet: ycin–glutamine (10378016, Gibco).Transfection and stable clone selection. Cells were transfected with 0.5 μg of a human DOT1L overexpression plasmid (RC218547, OriGene, Rockville, MD, USA) together with 3 μl HyFect (Leadgene, Tainan, Taiwan, ROC) and incubated for 2 days. Afterwards, the cells were reseeded into a 10 cm dish and selected with 1.6 mg/ml neom
Showing 1-2 of 2 papers.
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