NG2 (CSPG4) (NM_001897) Human Tagged ORF Clone
$2,874.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | NG2 |
| Synonyms | CSPG4A; HMW-MAA; MCSP; MCSPG; MEL-CSPG; MSK16; NG2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001897 |
| ORF Size | 6966 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001897.5 |
| RefSeq Size | 7896 bp |
| RefSeq ORF | 6969 bp |
| Locus ID | 1464 |
| UniProt ID | Q6UVK1 |
| Cytogenetics | 15q24.2 |
| Protein Families | Transmembrane |
| MW | 250.4 kDa |
| Summary | A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001897" in other vectors (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC218462L1 | Lenti ORF clone of Human chondroitin sulfate proteoglycan 4 (CSPG4), Myc-DDK-tagged | 10 ug |
$3,174.00
|
|
| RG218462 | CSPG4 (tGFP-tagged) - Human chondroitin sulfate proteoglycan 4 (CSPG4) | 10 ug |
$3,074.00
|
|
| SC303093 | CSPG4 (untagged)-Human chondroitin sulfate proteoglycan 4 (CSPG4) | 10 ug |
$2,875.00
|
|
Citations
Functional defects in Clostridium difficile TcdB toxin uptake identify CSPG4 receptor binding determinants
Gupta, Zhang, Sugiman-Marangos et al
J. Biol. Chem. (2017)
Gupta, Zhang, Sugiman-Marangos et al
J. Biol. Chem. (2017)
AI Summary: OriGene's pCMV6-CSPG4 vector (Cat# RC218462), expressing full-length Myc-DDK-tagged CSPG4, was used to generate CSPG4-expressing HEK 293 cell lines for studying TcdB toxin-receptor interactions in this paper.
Snippet: BioMax MR film (Kodak). Generation of CSPG4 expressing HEK 293 Cell Line—pCMV6-CSPG4 vector expressing full length Myc-DDK-tagged CSPG4 was purchased from OriGene Technologies (Cat# RC218462). HEK-293 cells ... Cite SaveSaving
Clostridium difficile Toxins: Entry and Uptake
Zhang
Thesis (2018)
Zhang
Thesis (2018)
Snippet: pCMV6-CSPG4 vector expressing full length Myc-DDK-tagged CSPG4 was purchased from OriGene Technologies (Cat# RC218462). HEK-293 cells were transfected with pCMV6-CSPG4 plasmid using Lipofectamine-2000 according to manufacturer's protocol (ThermoFisher Scientific)
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
