NSMase2 (SMPD3) (NM_018667) Human Tagged ORF Clone
SKU
RC218441
SMPD3 (Myc-DDK-tagged)-Human sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinase II) (SMPD3)
$610.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | NSMase2 |
| Synonyms | NSMASE2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_018667 |
| ORF Size | 1965 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_018667.2, NP_061137.1 |
| RefSeq Size | 5284 bp |
| RefSeq ORF | 1968 bp |
| Locus ID | 55512 |
| UniProt ID | Q9NY59 |
| Cytogenetics | 16q22.1 |
| Domains | Exo_endo_phos |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, Sphingolipid metabolism |
| MW | 70.9 kDa |
| Summary | Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_018667" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC218441L1 | Lenti ORF clone of Human sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinase II) (SMPD3), Myc-DDK-tagged | 10 ug |
$910.00
|
|
| RC218441L2 | Lenti ORF clone of Human sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinase II) (SMPD3), mGFP tagged | 10 ug |
$910.00
|
|
| RC218441L3 | Lenti ORF clone of Human sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinase II) (SMPD3), Myc-DDK-tagged | 10 ug |
$910.00
|
|
| RC218441L4 | Lenti ORF clone of Human sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinase II) (SMPD3), mGFP tagged | 10 ug |
$910.00
|
|
| RG218441 | SMPD3 (tGFP-tagged) - Human sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinase II) (SMPD3) | 10 ug |
$810.00
|
|
| SC314339 | SMPD3 (untagged)-Human sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinase II) (SMPD3) | 10 ug |
$672.00
|
|
Citations
Reduction of sphingomyelinase activity associated with progranulin deficiency and frontotemporal dementia
Boyle, Fox, Tadepalli et al
Neurobiol Dis (2025) 213, 107024 DOI: 10.1016/j.nbd.2025.107024
Boyle, Fox, Tadepalli et al
Neurobiol Dis (2025) 213, 107024 DOI: 10.1016/j.nbd.2025.107024
Cited by 4
AI Summary: OriGene products contributed to this study by providing plasmids expressing FLAG-tagged human ASMase (RC219758), FLAG-tagged human nSMase2 (RC218441), and HA-tagged human progranulin (derived from RC202139), which were used for transient transfection experiments to investigate the roles of these proteins in sphingomyelin metabolism associated with progranulin deficiency and frontotemporal dementia.
Snippet: For transient transfections, plasmids containing the relevant constructs were diluted in Opti-MEM (Gibco) and incubated with Fugene (Promega) for 15 min at room temperature before being directly added to the wells. Plasmids expressing FLAG-tagged human ASMase (Origene RC219758), FLAG-tagged human nSMase2 (Origene RC218441), and HA-tagged human progranulin (derived from Origene RC202139) were used
Allosteric Inhibition of Neutral Sphingomyelinase 2 (nSMase2) by DPTIP: From Antiflaviviral Activity to Deciphering Its Binding Site through In Silico Studies and Experimental Validation
Álvarez-Fernández, Mingo-Casas, Blázquez et al
Int J Mol Sci (2022) 23 (22) DOI: 10.3390/ijms232213935
Álvarez-Fernández, Mingo-Casas, Blázquez et al
Int J Mol Sci (2022) 23 (22) DOI: 10.3390/ijms232213935
Cited by 14
AI Summary: OriGene’s human NSMase2 (SMPD3) expression plasmid (Catalog # RC218441) was used to produce wild-type and mutant nSMase2 proteins, enabling experimental validation of DPTIP’s inhibitory mechanism in the study.
Snippet: se2 (SMPD3) was produced using an expression plasmid that encodes the cDNA of human NSMase2 (SMPD3) (NM_018667) fused to Myc-DDk tag cloned between SgfI-MluI restricition sites of pCMV6-Entry vector (OriGene technologies, Inc., Rockville, MD, USA, Catalog # RC218441). Plasmid encoding mutant nSMAse2 with amino acid replacement histidine 463 to alanine (H463A) was produced by directed mutagenesis w
A novel and potent brain penetrant inhibitor of extracellular vesicle release
Rojas, Sala, Thomas et al
Br. J. Pharmacol. (2019)
Rojas, Sala, Thomas et al
Br. J. Pharmacol. (2019)
AI Summary: OriGene contributed the pCMV6 entry vector containing a C-terminal Flag-tagged SMPD3 gene, which was used to transfect HEK293 cells in this study.
Snippet: Materials The pCMV6 entry vector containing C-terminal Flag-tagged SMPD3 gene used to transfect into HEK293 cells [RRID: CVCL_0045] was obtained from Origene (Rockville, MD, USA). Sphingomyelin (SM) and Amplex Red reagent were purchased from Life Technologies
DPTIP, a newly identified potent brain penetrant neutral sphingomyelinase 2 inhibitor, regulates astrocyte-peripheral immune communication following brain inflammation
Rojas, Barnaeva, Thomas et al
Sci Rep (2018) 8 (1), 17715 DOI: 10.1038/s41598-018-36144-2
Rojas, Barnaeva, Thomas et al
Sci Rep (2018) 8 (1), 17715 DOI: 10.1038/s41598-018-36144-2
AI Summary: OriGene’s full-length human nSMase2 cDNA with a C-terminal Flag tag cloned into the pCMV6-Entry expression vector was used to express nSMase2 in HEK293 cells, enabling the characterization and screening of nSMase2 inhibitors in this study.
Snippet: … Methods Expression of human nSMase2 Full length human nSMase2 cDNA with a C-terminal Flag tag cloned into a pCMV6-Entry expression vector (Origene) was transfected into HEK293 cells using lipofectamine 2000 (Life Technologies). Selection of transfected cells was carried out for two weeks with 500 µg/ml G418 in EMEM containing 10% FBS;Full length human nSMase2 cDNA with a C-terminal Flag tag cloned into a pCMV6-Entry expression vector (Origene) was transfected into HEK293 cells using lipofectamine 2000 (Life Technologies). …
Involvement of Cryptosporidium parvum Cdg7_FLc_1000 RNA in the Attenuation of Intestinal Epithelial Cell Migration via Trans-Suppression of Host Cell SMPD3
Ming, Gong, Wang et al
J Infect Dis (2017) 217 (1), 122-133 DOI: 10.1093/infdis/jix392
Ming, Gong, Wang et al
J Infect Dis (2017) 217 (1), 122-133 DOI: 10.1093/infdis/jix392
Cited by 19
AI Summary: In this study, OriGene’s SMPD3 Human cDNA ORF Clone was used to investigate the role of SMPD3 in mediating the effects of Cryptosporidium parvum Cdg7_FLc_1000 RNA on intestinal epithelial cell migration.
The cationic small molecule GW4869 is cytotoxic to high phosphatidylserine-expressing myeloma cells
Vuckovic, Vandyke, Rickards et al
Br. J. Haematol. (2017)
Vuckovic, Vandyke, Rickards et al
Br. J. Haematol. (2017)
Showing 1-6 of 7 papers.
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