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ZNF750 (NM_024702) Human Tagged ORF Clone

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SKU
RC218356
ZNF750 (Myc-DDK-tagged)-Human zinc finger protein 750 (ZNF750)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $662.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "ZNF750 or NM_024702"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ZNF750
Synonyms ZFP750
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC218356 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTCTCCTCAAAGAGCGGAAGCCAAAAAAGCCACATTACATCCCCAGGCCTCCAGGAAAGCCCTTCA
AGTATAAATGTTTCCAATGTCCCTTTACTTGCAATGAGAAGTCACATCTTTTTAATCACATGAAGTATGG
TCTTTGTAAAAACTCGATTACTTTAGTATCAGAGCAGGATCGAGTTCCCAAGTGCCCTAAATCTAACTCA
CTAGACCCCAAGCAAACCAACCAGCCCGATGCCACGGCGAAGCCAGCCTCTTCCAAGTCTGTCGCAAATG
GACTCTCTGCCTTCGACTCGAAGCTTCAGCACAGCTCTGCCAGGGAAGACATCAAGGAAAACCTGGAGCT
GCAAGCCCGGGGAACCCACAGGTGCCTGGGACAGAAGCCAGCCCTCCACAGGGCATCACCCTGCAAGAGC
CCAGCTCCGGAAGCCGCCCTCGGTGCCCAGCCTGCTCTGGAAGGCGCAGCTCGGCCTTCTGCATTTGTTC
CAGTCGGCGAGCACAGACTCAAGGGGCCAGACAACGCCGAGGCGCCCGAGACACTGGCTTTACACAACCC
CACTGCCAAGGCCGTGTCTTTCCACACCAAGTCGGCCTTCCACACTCCTGGCTACCCCTGGAAAGCCGGC
TCACCTTTCCTTCCACCAGAGTTTCCACATAAAATCTCATCTACAAAGGGGCTTGGGGCCATTTCCCCTT
ACATGCACCCCACAATCCCAGAGTACCCGCCTCACTTTTACACAGAGCACGGGCTGGCCACCATCTACTC
GCCTTACCTGCTGGCTGGGAGCTCGCCTGAGTGTGACGCACCCCTGCTGTCAGTCTACGGAACCCAAGAC
CCGAGACACTTCCTGCCTCACCCGGGGCCGATCCCTAAGCACCTGGCTCCATCTCCAGCCACATACGATC
ACTACAGGTTTTTCCAGCAATATCCCTCTAACCTGCCGATTCCTTACGGATTTTACAGGCCAGAGTCTGC
ATTTTCCTCCTATGGTCTCAGACTCCCACCTGTCACTGGCCTCACCCGAGATCAGAGCTCTCACCTGCTT
GAAGAAGCCACCCTGGTCTATCCAGCCTCGAGTCCTTCCAGGTTAAACCCTTCGGACCCCAACAGAAAAC
ACGTCGAGTTCGAAAGTCCAATTCCTGAGGCTAAAGACTCCTCCAAGGCTGGGCAGAGAGACACGGAAGG
GTCCAAAATGAGCCCCCGCGCAGGGAGTGCAGCCACGGGCTCCCCAGGGAGGCCGAGCCCCACCGACTTC
ATGCAGACGAGCCAGACCTGCGAAGGCCTGTACGACCTCTCCAACAAGGCAGCCTCCAGCGCACTGGGAA
GACTCTACCCGCCAGAGCAAAGCCTCACAGCCTTCAGGCCTGTTAAGAAAAGCACAGAATGCCTACCTGC
CCAGGCTGCTGAGACCACAGCAGAGTCTCCAGTAAGCCTCAATGTTGTGAACGGAGACCCTCCTGCTCCG
ACCGGAAGCGCCTCTCTCGTCTCGGAGGCCGCGCCTTCCAGTCCGGACGACAGCTCCGGGATGGGCCCCC
TCAACCTCTCCAAGAAATCAGAGATAAACCTGGCAGCCACCCACGAACCCACGTACCAAGGCAGCCCCCA
GGCGGAAACCGCCAGCTTCTCAGAGCTGCAGGACCTTCCACTCAATCTCTCGGTGAAGGACCCCTGTAAC
ACCCAGGCTCCGAGGCCCGCCTTCCCCGGTCGACCACGAGCTGCGGAACCTGCTGCTGCTGTTCCACAGA
AGACTGGGACAGAAGGTTCTGAGGATGGGCCCAGCCACCCTGAGACCAAGCCAGGCAGCCTCGACGGTGA
CGGGGCCCCACCCACAGGCCCCGGCGAGGAGGCTCCAGACGCATGCGCGGTGGACAGCAGCGAGGAGCAG
AAGCAGACGGCAGCCGTGGCCCTGTGCCAGCTGGCGGCCTACAGCCCCAGGAACATCCGGGTGGGCGATG
GGGATGCTGCGGCCCCGGAACCTGCCTGCCGGCAAGACACACCCACACTGAGCTCCATGGAGAGCCAAGA
GGCCCAGTGTGACCTCAGACCCAAAGGACAAAAGAGGACAAGTCTAAGGGATGCTGGAAAATCCCAGCAA
GGAGCTAAGAAGGCGAAGCTGCAGGACACGGCCAGAGTGTTCACACTACGAAGGAGGGCCCGGGTGTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC218356 protein sequence
Red=Cloning site Green=Tags(s)

MSLLKERKPKKPHYIPRPPGKPFKYKCFQCPFTCNEKSHLFNHMKYGLCKNSITLVSEQDRVPKCPKSNS
LDPKQTNQPDATAKPASSKSVANGLSAFDSKLQHSSAREDIKENLELQARGTHRCLGQKPALHRASPCKS
PAPEAALGAQPALEGAARPSAFVPVGEHRLKGPDNAEAPETLALHNPTAKAVSFHTKSAFHTPGYPWKAG
SPFLPPEFPHKISSTKGLGAISPYMHPTIPEYPPHFYTEHGLATIYSPYLLAGSSPECDAPLLSVYGTQD
PRHFLPHPGPIPKHLAPSPATYDHYRFFQQYPSNLPIPYGFYRPESAFSSYGLRLPPVTGLTRDQSSHLL
EEATLVYPASSPSRLNPSDPNRKHVEFESPIPEAKDSSKAGQRDTEGSKMSPRAGSAATGSPGRPSPTDF
MQTSQTCEGLYDLSNKAASSALGRLYPPEQSLTAFRPVKKSTECLPAQAAETTAESPVSLNVVNGDPPAP
TGSASLVSEAAPSSPDDSSGMGPLNLSKKSEINLAATHEPTYQGSPQAETASFSELQDLPLNLSVKDPCN
TQAPRPAFPGRPRAAEPAAAVPQKTGTEGSEDGPSHPETKPGSLDGDGAPPTGPGEEAPDACAVDSSEEQ
KQTAAVALCQLAAYSPRNIRVGDGDAAAPEPACRQDTPTLSSMESQEAQCDLRPKGQKRTSLRDAGKSQQ
GAKKAKLQDTARVFTLRRRARVS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024702
ORF Size 2169 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024702.3
RefSeq Size 3218 bp
RefSeq ORF 2172 bp
Locus ID 79755
UniProt ID Q32MQ0
Cytogenetics 17q25.3
Domains zf-C2H2
MW 77.4 kDa
Summary This gene encodes a protein with a nuclear localization site and a C2H2 zinc finger domain. Mutations in this gene have been associated with seborrhea-like dermatitis with psoriasiform elements. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_024702" in other vectors (6)
SKU Description Size Price
RC218356L1 Lenti ORF clone of Human zinc finger protein 750 (ZNF750), Myc-DDK-tagged 10 ug
$962.00
RC218356L2 Lenti ORF clone of Human zinc finger protein 750 (ZNF750), mGFP tagged 10 ug
$962.00
RC218356L3 Lenti ORF clone of Human zinc finger protein 750 (ZNF750), Myc-DDK-tagged 10 ug
$962.00
RC218356L4 Lenti ORF clone of Human zinc finger protein 750 (ZNF750), mGFP tagged 10 ug
$962.00
RG218356 ZNF750 (tGFP-tagged) - Human zinc finger protein 750 (ZNF750) 10 ug
$789.00 $862.00
SC112082 ZNF750 (untagged)-Human zinc finger protein 750 (ZNF750) 10 ug
$663.00

Plasmid Map for RC218356

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC218356
Circular map for RC218356

file-alt Citations

ZNF750 represses breast cancer invasion via epigenetic control of prometastatic genes
Cassandri, Butera, Amelio et al
Oncogene (2020) 39 (22), 4331-4343 DOI: 10.1038/s41388-020-1277-5
Cited by 81
AI Summary: OriGene products contributed to this study by providing the ZNF750 cDNA clone (Cat# RC217307) and ZNF750-specific shRNA (Cat# TL314863), which were used to manipulate ZNF750 expression and investigate its role in repressing breast cancer cell migration and invasion.
Snippet: … Here, we show that zinc-finger protein 750 (ZNF750) opposes the migration and invasion;- Breast cancer is the second leading cause of cancer-related deaths among women, largely due to the progression of a significant fraction of primary tumours to the metastatic stage. Here, we show that zinc-finger protein 750 (ZNF750) opposes the migration and invasion;narico%2CAG&author=Giacobbe%2CA&author=Peschiaroli%2CA&author=Frezza%2CV&author=Cicalese%2CA] * 12. …
Showing 1-1 of 1 papers.
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