PNR (TAAR5) (NM_003967) Human Tagged ORF Clone

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SKU
RC218295
TAAR5 (Myc-DDK-tagged)-Human trace amine associated receptor 5 (TAAR5)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PNR
Synonyms PNR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC218295 representing NM_003967
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGAGCTGTCTTCATCCAAGGTGCTGAAGAGCACCCTGCGGCATTCTGCTACCAGGTGAATGGGTCTT
GCCCCAGGACAGTACATACTCTGGGCATCCAGTTGGTCATCTACCTGGCCTGTGCAGCAGGCATGCTGAT
TATCGTGCTAGGGAATGTATTTGTGGCATTTGCTGTGTCCTACTTCAAAGCGCTTCACACGCCCACCAAC
TTCCTGCTGCTCTCCCTGGCCCTGGCTGACATGTTTCTGGGTCTGCTGGTGCTGCCCCTCAGCACCATTC
GCTCAGTGGAGAGCTGCTGGTTCTTCGGGGACTTCCTCTGCCGCCTGCACACCTACCTGGACACCCTCTT
CTGCCTCACCTCCATCTTCCATCTCTGTTTCATTTCCATTGACCGCCACTGTGCCATCTGTGACCCCCTG
CTCTATCCCTCCAAGTTCACAGTGAGGGTGGCTCTCAGGTACATCCTGGCAGGATGGGGGGTGCCCGCAG
CATACACTTCGTTATTCCTCTACACAGATGTGGTAGAGACAAGGCTCAGCCAGTGGCTGGAAGAGATGCC
TTGTGTGGGCAGTTGCCAGCTGCTGCTCAATAAATTTTGGGGCTGGTTAAACTTCCCTTTGTTCTTTGTC
CCCTGCCTCATTATGATCAGCTTGTATGTGAAGATCTTTGTGGTTGCTACCAGACAGGCTCAGCAGATTA
CCACATTGAGCAAAAGCCTGGCTGGGGCTGCCAAGCATGAGAGAAAAGCTGCCAAGACCCTGGGCATTGC
TGTGGGCATATACCTCTTGTGCTGGCTGCCCTTCACCATAGACACGATGGTCGACAGCCTCCTTCACTTT
ATCACACCCCCACTGGTCTTTGACATCTTTATCTGGTTTGCTTACTTCAACTCAGCCTGCAACCCCATCA
TCTATGTCTTTTCCTACCAGTGGTTTCGGAAGGCACTGAAACTCACACTGAGCCAGAAGGTCTTCTCACC
GCAGACACGCACTGTTGATTTGTACCAAGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC218295 representing NM_003967
Red=Cloning site Green=Tags(s)

MRAVFIQGAEEHPAAFCYQVNGSCPRTVHTLGIQLVIYLACAAGMLIIVLGNVFVAFAVSYFKALHTPTN
FLLLSLALADMFLGLLVLPLSTIRSVESCWFFGDFLCRLHTYLDTLFCLTSIFHLCFISIDRHCAICDPL
LYPSKFTVRVALRYILAGWGVPAAYTSLFLYTDVVETRLSQWLEEMPCVGSCQLLLNKFWGWLNFPLFFV
PCLIMISLYVKIFVVATRQAQQITTLSKSLAGAAKHERKAAKTLGIAVGIYLLCWLPFTIDTMVDSLLHF
ITPPLVFDIFIWFAYFNSACNPIIYVFSYQWFRKALKLTLSQKVFSPQTRTVDLYQE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003967
ORF Size 1011 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003967.2
RefSeq Size 1014 bp
RefSeq ORF 1014 bp
Locus ID 9038
UniProt ID O14804
Cytogenetics 6q23.2
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
MW 38.1 kDa
Summary Olfactory receptor specific for trimethylamine, a trace amine. Also activated at lower level by dimethylethylamine. Trimethylamine is a bacterial metabolite found in some animal odors, and to humans it is a repulsive odor associated with bad breath and spoiled food. This receptor is probably mediated by the G(s)-class of G-proteins which activate adenylate cyclase.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_003967" in other vectors (6)
SKU Description Size Price
RC218295L1 Lenti ORF clone of Human trace amine associated receptor 5 (TAAR5), Myc-DDK-tagged 10 ug
$757.00
RC218295L2 Lenti ORF clone of Human trace amine associated receptor 5 (TAAR5), mGFP tagged 10 ug
$757.00
RC218295L3 Lenti ORF clone of Human trace amine associated receptor 5 (TAAR5), Myc-DDK-tagged 10 ug
$757.00
RC218295L4 Lenti ORF clone of Human trace amine associated receptor 5 (TAAR5), mGFP tagged 10 ug
$757.00
RG218295 TAAR5 (tGFP-tagged) - Human trace amine associated receptor 5 (TAAR5) 10 ug
$489.00 $657.00
SC117662 TAAR5 (untagged)-Human trace amine associated receptor 5 (TAAR5) 10 ug
$457.00

Plasmid Map for RC218295

Download sequence for

Cloning scheme for RC218295

Circular map for RC218295

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