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PALM2AKAP2 (NM_001004065) Human Tagged ORF Clone

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SKU
RC217988
AKAP2 (Myc-DDK-tagged)-Human A kinase (PRKA) anchor protein 2 (AKAP2), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $868.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PALM2AKAP2
Synonyms AKAP-2; AKAP-KL; AKAP2; AKAPKL; MISP2; PALM2; PALM2-AKAP2; PRKA2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC217988 representing NM_001004065
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCTGGCCCCAGCCCGGGGCTGCCGCTCGCCTTCCCCCGGAGTCTCCTGGACCCCCGGAGTCTCCTG
GACCCCCGGAGCGGGAGGCAGCGGCCGCGCGGCGCTGGACTGGAGCAGAACCCCAGGACTGCGCCCCCGG
GAGCGGGCGCCCAGAGAAGCCTCCCCAGCTTTCTGAGGATGATATCTGGCTAAAAAGCGAGGGAGACAAC
TATAGTGCCACCCTCCTGGAGCCTGCTGCCAGCTCTCTTTCCCCAGATCACAAAAACATGGAAATTGAGG
TGTCTGTTGCAGAATGTAAAAGTGTTCCTGGAATCACCTCTACCCCACATCCCATGGACCATCCCTCCGC
TTTCTATTCACCCCCGCATAATGGCCTCCTTACTGATCACCACGAATCCCTGGATAATGATGTTGCCAGA
GAGATCCGCTATCTAGATGAGGTGCTAGAGGCCAACTGCTGTGATTCTGCTGTGGATGGAACGTACAATG
GAACATCCTCCCCAGAGCCTGGTGCAGTGGTTCTGGTGGGCGGCCTAAGCCCCCCTGTCCACGAGGCGAC
CCAGCCAGAACCCACTGAAAGAACAGCTAGCCGGCAGGCACCTCCTCACATCGAGCTCAGTAATAGCAGC
CCTGACCCCATGGCAGAGGCAGAAAGAACAAATGGCCATTCCCCCAGCCAGCCTAGAGATGCGCTGGGGG
ACAGCCTGCAGGTGCCTGTCAGCCCCAGCTCCACAACCAGCTCACGGTGTTCTTCCCGAGATGGAGAGTT
CACTCTCACCACACTGAAAAAGGAGGCCAAGTTTGAGCTGCGTGCCTTCCATGAGGACAAGAAGCCCTCC
AAGCTCTTTGAGGATGACGAGCATGAGAAAGAACAATACTGCATTAGAAAAGTGAGGCCTTCAGAGGAGA
TGCTGGAGCTGGAAAAGGAGAGGAGAGAGCTCATCCGCAGCCAGGCCGTCAAGAAGAATCCTGGCATTGC
AGCAAAATGGTGGAATCCCCCGCAGGAAAAAACCATCGAGGAGCAGCTGGACGAGGAACATCTGGAGTCG
CACAAAAAGTACAAGGAGCGCAAAGAGAGAAGGGCACAGCAGGAACAGTTGCTGCTGCAGAAGCAGTTAC
AGCAGCAGCAGCAGCAGCCCCCATCGCAGCTCTGCACAGCCCCTGCCTCTTCTCATGAACGCGCAAGCAT
GATTGACAAAGCAAAGGAGGACATTGTCACAGAGCAGATAGATTTCTCTGCTGCTCGCAAACAATTTCAG
CTGATGGAGAATTCCAGGCAAGCGGTGGCCAAGGGCCAGAGTACACCCAGGCTGTTCTCCATCAAGCCTT
TCTACAGGCCTCTGGGGTCAGTCAACTCAGACAAGCCACTGACTAATCCGAGACCACCTTCTGTCGGGGG
ACCTCCAGAAGACAGTGGTGCCTCAGCCGCCAAGGGACAGAAATCCCCCGGTGCCCTGGAGACCCCATCG
GCAGCAGGAAGCCAGGGCAACACAGCCTCTCAGGGGAAGGAAGGGCCCTACAGCGAGCCTTCTAAACGTG
GGCCCTTATCTAAACTGTGGGCTGAGGATGGAGAATTTACGAGCGCCCGGGCTGTCCTCACTGTGGTCAA
GGATGATGACCATGGGATTTTGGATCAGTTCTCAAGATCTGTCAATGTCTCCTTGACCCAAGAGGAGCTT
GACTCTGGTCTGGACGAATTGTCGGTGAGGTCTCAGGATACCACAGTCCTGGAGACCCTATCCAATGATT
TCAGCATGGACAACATCAGTGACAGCGGGGCATCCAATGAGACAACCAATGCCCTCCAGGAAAATTCACT
GGCTGATTTTTCTCTGCCCCAGACACCACAAACTGACAACCCCTCAGAGGGCCGAGGAGAAGGCGTCTCC
AAGTCATTTAGTGATCATGGTTTCTATTCCCCTTCCTCCACGCTGGGGGACTCTCCGTTGGTTGATGACC
CCTTGGAGTATCAGGCTGGCCTCCTGGTGCAGAATGCCATTCAACAAGCCATAGCCGAGCAGGTGGATAA
AGCTGTGTCCAAAACCAGCAGGGATGGAGCAGAGCAACAGGGACCTGAAGCGACTGTAGAGGAAGCTGAA
GCTGCGGCTTTCGGCTCAGAAAAGCCTCAGAGCATGTTTGAGCCACCTCAGGTGTCTTCTCCTGTTCAAG
AGAAAAGGGATGTATTACCAAAGATTCTGCCTGCTGAAGACAGGGCGCTCAGGGAAAGGGGGCCCCCCCA
GCCACTGCCAGCTGTGCAGCCCAGTGGCCCGATTAACATGGAGGAGACCAGGCCCGAAGGAAGCTATTTC
AGCAAGTACTCGGAGGCAGCTGAGCTGAGAAGCACAGCCTCCCTCCTGGCCACTCAAGAATCTGACGTGA
TGGTTGGGCCTTTCAAGCTGAGGTCCAGGAAACAGCGGACTTTGTCCATGATTGAGGAAGAGATCCGAGC
AGCTCAGGAAAGGGAAGAGGAGCTGAAGAGGCAGAGACAAGTCTTGCAGAGTACGCAGAGCCCCAGGACA
AAGAATGCCCCATCACTGCCCTCCAGAACATGCTACAAAACTGCTCCAGGGAAAATAGAGAAAGTCAAAC
CTCCTCCATCCCCCACCACTGAAGGCCCCAGCTTGCAGCCTGACTTAGCCCCTGAAGAGGCTGCCGGAAC
CCAGCGGCCCAAGAATCTGATGCAGACCCTCATGGAAGACTATGAGACACACAAATCTAAAAGGCGCGAG
AGAATGGATGATAGTAGTGTCCTCGAGGCCACACGGGTTAATCGAAGAAAGAGCGCACTGGCTTTGCGCT
GGGAAGCAGGGATCTATGCCAACCAGGAGGAAGAAGACAACGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC217988 representing NM_001004065
Red=Cloning site Green=Tags(s)

MRWPQPGAAARLPPESPGPPESPGPPEREAAAARRWTGAEPQDCAPGSGRPEKPPQLSEDDIWLKSEGDN
YSATLLEPAASSLSPDHKNMEIEVSVAECKSVPGITSTPHPMDHPSAFYSPPHNGLLTDHHESLDNDVAR
EIRYLDEVLEANCCDSAVDGTYNGTSSPEPGAVVLVGGLSPPVHEATQPEPTERTASRQAPPHIELSNSS
PDPMAEAERTNGHSPSQPRDALGDSLQVPVSPSSTTSSRCSSRDGEFTLTTLKKEAKFELRAFHEDKKPS
KLFEDDEHEKEQYCIRKVRPSEEMLELEKERRELIRSQAVKKNPGIAAKWWNPPQEKTIEEQLDEEHLES
HKKYKERKERRAQQEQLLLQKQLQQQQQQPPSQLCTAPASSHERASMIDKAKEDIVTEQIDFSAARKQFQ
LMENSRQAVAKGQSTPRLFSIKPFYRPLGSVNSDKPLTNPRPPSVGGPPEDSGASAAKGQKSPGALETPS
AAGSQGNTASQGKEGPYSEPSKRGPLSKLWAEDGEFTSARAVLTVVKDDDHGILDQFSRSVNVSLTQEEL
DSGLDELSVRSQDTTVLETLSNDFSMDNISDSGASNETTNALQENSLADFSLPQTPQTDNPSEGRGEGVS
KSFSDHGFYSPSSTLGDSPLVDDPLEYQAGLLVQNAIQQAIAEQVDKAVSKTSRDGAEQQGPEATVEEAE
AAAFGSEKPQSMFEPPQVSSPVQEKRDVLPKILPAEDRALRERGPPQPLPAVQPSGPINMEETRPEGSYF
SKYSEAAELRSTASLLATQESDVMVGPFKLRSRKQRTLSMIEEEIRAAQEREEELKRQRQVLQSTQSPRT
KNAPSLPSRTCYKTAPGKIEKVKPPPSPTTEGPSLQPDLAPEEAAGTQRPKNLMQTLMEDYETHKSKRRE
RMDDSSVLEATRVNRRKSALALRWEAGIYANQEEEDNE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001004065
ORF Size 2844 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001004065.4, NP_001004065.2
RefSeq Size 6866 bp
RefSeq ORF 2847 bp
Locus ID 445815
UniProt ID Q9Y2D5
Cytogenetics 9q31.3
MW 103.9 kDa
Summary This gene belongs to the paralemmin downstream gene (PDG) family defined in PMID:22855693. Paralemmin downstream genes may have evolved contiguously with the paralemmin genes and are associated with other paralemmin paralogs in humans and several other taxa. The gene encodes three distinct protein isoforms, the PALM2 isoform, the AKAP2 isoform and the PALM2-AKAP2 isoform. The biological significance of the PALM2-AKAP2 isoforms is yet unknown. Earlier, PALM2 and AKAP2 were annotated as separate genes and PALM2-AKAP2 was annotated as a readthrough gene. provided by RefSeq, May 2019
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Documents
Resources
"NM_001004065" in other vectors (4)
SKU Description Size Price
RC217988L3 Lenti-ORF clone of AKAP2 (Myc-DDK-tagged)-Human A kinase (PRKA) anchor protein 2 (AKAP2), transcript variant 1 10 ug
$1,168.00
RC217988L4 Lenti-ORF clone of AKAP2 (mGFP-tagged)-Human A kinase (PRKA) anchor protein 2 (AKAP2), transcript variant 1 10 ug
$1,168.00
RG217988 AKAP2 (tGFP-tagged) - Human A kinase (PRKA) anchor protein 2 (AKAP2), transcript variant 1 10 ug
$1,068.00
SC300599 AKAP2 (untagged)-Human A kinase (PRKA) anchor protein 2 (AKAP2), transcript variant 1 10 ug
$956.00

Plasmid Map for RC217988

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC217988
Circular map for RC217988

file-alt Citations

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