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PBX4 (NM_025245) Human Tagged ORF Clone

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SKU
RC217742
PBX4 (Myc-DDK-tagged)-Human pre-B-cell leukemia homeobox 4 (PBX4), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PBX4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC217742 representing NM_025245
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCCCCGCCGCGCCCCGCGCCATCGCCCCCCGCCCCGCGGCGCCTCGACACGAGCGACGTCCTGC
AGCAGATCATGGCCATCACCGACCAGAGCCTGGACGAGGCACAGGCCAGAAAGCATGCTCTGAATTGCCA
TCGGATGAAGCCTGCTCTGTTCAGCGTGCTCTGTGAGATCAAGGAAAAGACAGTGGTAAGCATCCGTGGC
ATTCAAGACGAAGATCCCCCTGACGCCCAGCTCCTGAGGCTGGATAACATGCTGCTGGCTGAGGGCGTGT
GCAGGCCCGAGAAGAGAGGAAGAGGAGGAGCGGTGGCCAGGGCCGGCACAGCAACACCAGGTGGCTGTCC
AAATGACAATAGCATTGAGCACTCTGACTACAGGGCCAAGCTGTCCCAGATCCGACAGATTTACCACTCT
GAGCTAGAGAAATATGAACAGGCCTGTCGTGAGTTCACCACGCACGTCACCAACCTCCTCCAGGAGCAGA
GCAGGATGAGGCCTGTCTCCCCTAAGGAGATTGAGCGCATGGTCGGCGCCATTCACGGCAAGTTCAGCGC
CATCCAGATGCAGTTGAAGCAGAGCACCTGTGAGGCAGTGATGACCCTGCGTTCGCGGCTGCTCGATGCC
AGGCGCAAGCGGCGGAATTTCAGCAAGCAGGCGACGGAAGTGCTGAATGAGTATTTTTACTCCCATCTGA
ACAACCCTTACCCCAGCGAAGAAGCCAAAGAAGAGCTGGCCAGGAAGGGCGGCCTCACCATCTCCCAGGT
CTCTAACTGGTTTGGCAACAAAAGAATCCGGTATAAAAAGAACATGGGGAAGTTTCAAGAAGAGGCTACC
ATTTACACGGGTAAAACGGCTGTGGATACCACGGAAGTTGGGGTCCCAGGGAACCACGCCAGCTGCCTGT
CAACACCTAGCTCCGGCTCCTCTGGACCCTTCCCGCTGCCCAGCGCTGGGGACGCCTTCCTCACCCTGCG
GACTCTGGCCTCTCTCCAGCCTCCTCCTGGGGGAGGCTGCCTGCAGTCCCAGGCCCAGGGTAGCTGGCAG
GGGGCCACCCCCCAACCTGCAACTGCCTCACCTGCTGGAGACCCTGGCAGCATCAACTCCAGTACATCTA
AT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC217742 representing NM_025245
Red=Cloning site Green=Tags(s)

MAAPPRPAPSPPAPRRLDTSDVLQQIMAITDQSLDEAQARKHALNCHRMKPALFSVLCEIKEKTVVSIRG
IQDEDPPDAQLLRLDNMLLAEGVCRPEKRGRGGAVARAGTATPGGCPNDNSIEHSDYRAKLSQIRQIYHS
ELEKYEQACREFTTHVTNLLQEQSRMRPVSPKEIERMVGAIHGKFSAIQMQLKQSTCEAVMTLRSRLLDA
RRKRRNFSKQATEVLNEYFYSHLNNPYPSEEAKEELARKGGLTISQVSNWFGNKRIRYKKNMGKFQEEAT
IYTGKTAVDTTEVGVPGNHASCLSTPSSGSSGPFPLPSAGDAFLTLRTLASLQPPPGGGCLQSQAQGSWQ
GATPQPATASPAGDPGSINSSTSN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025245
ORF Size 1122 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025245.3
RefSeq Size 1443 bp
RefSeq ORF 1125 bp
Locus ID 80714
UniProt ID Q9BYU1
Cytogenetics 19p13.11
Protein Families Transcription Factors
MW 40.7 kDa
Summary This gene encodes a member of the pre-B cell leukemia transcription factor family. These proteins are homeobox proteins that play critical roles in embryonic development and cellular differentiation both as Hox cofactors and through Hox-independent pathways. The encoded protein contains a homeobox DNA-binding domain, but specific functions of the protein have not been determined. Alternatively spliced transcript variants have been observed for this gene. provided by RefSeq, May 2011
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"NM_025245" in other vectors (6)
SKU Description Size Price
RC217742L1 Lenti ORF clone of Human pre-B-cell leukemia homeobox 4 (PBX4), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC217742L2 Lenti ORF clone of Human pre-B-cell leukemia homeobox 4 (PBX4), transcript variant 1, mGFP tagged 10 ug
$757.00
RC217742L3 Lenti ORF clone of Human pre-B-cell leukemia homeobox 4 (PBX4), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC217742L4 Lenti ORF clone of Human pre-B-cell leukemia homeobox 4 (PBX4), transcript variant 1, mGFP tagged 10 ug
$757.00
RG217742 PBX4 (tGFP-tagged) - Human pre-B-cell leukemia homeobox 4 (PBX4), transcript variant 1 10 ug
$489.00 $657.00
SC305251 PBX4 (untagged)-Human pre-B-cell leukemia homeobox 4 (PBX4), transcript variant 1 10 ug
$503.00

Plasmid Map for RC217742

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC217742
Circular map for RC217742

file-alt Citations

PBX4 functions as a potential novel oncopromoter in colorectal cancer: a comprehensive analysis of the PBX gene family
Martinou, Moller-Levet, Angelidi
Am J Cancer Res (2022) 12 (2), 585-600
AI Summary: OriGene’s pCMV6-AC-PBX4-GFP plasmid vector was used to overexpress PBX4 in colorectal cancer cells, enabling the study of PBX4’s role in cancer cell proliferation and gene expression changes.
Snippet: Plasmid vectors tagged with Green Fluorescence Protein (GFP) (OriGene, Germany) were used in the overexpression mechanistic studies. The pCMV6-AC-PBX4-GFP (
Showing 1-1 of 1 papers.
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