RHBG (NM_020407) Human Tagged ORF Clone

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SKU
RC217600
RHBG (Myc-DDK-tagged)-Human Rh family, B glycoprotein (gene/pseudogene) (RHBG), transcript variant 1
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RHBG
Synonyms SLC42A2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC217600 representing NM_020407
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGGGTCTCCTAGCCGCGCCGCGGGCCGGCGACTGCAGCTTCCCCTGCTGTGCCTCTTCCTCCAGG
GCGCCACTGCCGTCCTCTTTGCTGTCTTTGTCCGCTACAACCACAAAACCGACGCTGCCCTCTGGCACCG
GAGCAACCACAGTAACGCGGACAATGAATTTTACTTTCGCTACCCAAGCTTCCAGGACGTGCATGCCATG
GTCTTCGTGGGCTTTGGCTTCCTCATGGTCTTCCTGCAGCGTTACGGCTTCAGCAGCGTGGGCTTCACCT
TCCTCCTGGCCGCCTTTGCCCTGCAGTGGTCCACACTGGTCCAGGGCTTTCTCCACTCCTTCCACGGTGG
CCACATCCATGTTGGCGTGGAGAGCATGATCAATGCTGACTTTTGTGCGGGGGCCGTGCTCATCTCCTTT
GGTGCCGTCCTGGGCAAGACCGGGCCTACCCAGCTGCTGCTCATGGCCCTGCTGGAGGTGGTGCTGTTTG
GCATCAATGAGTTTGTGCTCCTTCATCTCCTGGGGGTGAGAGATGCCGGAGGCTCCATGACTATCCACAC
CTTTGGTGCCTACTTCGGGCTCGTCCTTTCGCGGGTTCTGTACAGGCCCCAGCTGGAGAAGAGCAAGCAC
CGCCAGGGCTCCGTCTACCATTCAGACCTCTTCGCCATGATTGGGACCATCTTCCTGTGGATCTTCTGGC
CTAGCTTCAATGCTGCACTCACAGCGCTGGGGGCTGGGCAGCATCGGACGGCCCTCAACACATACTACTC
CCTGGCTGCCAGCACCCTTGGCACCTTTGCCTTGTCAGCCCTTGTAGGGGAAGATGGGAGGCTTGACATG
GTCCACATCCAAAATGCAGCGCTGGCTGGAGGGGTTGTGGTGGGGACCTCAAGTGAAATGATGCTGACAC
CCTTTGGGGCTCTGGCAGCTGGCTTCTTGGCTGGGACTGTCTCCACGCTGGGGTACAAGTTCTTCACGCC
CATCCTTGAATCAAAATTCAAAGTCCAAGACACATGTGGAGTCCACAACCTCCATGGGATGCCGGGGGTC
CTGGGGGCCCTCCTGGGGGTCCTTGTGGCTGGACTTGCCACCCATGAAGCTTACGGAGATGGCCTGGAGA
GTGTGTTTCCACTCATAGCCGAGGGCCAGCGCAGTGCCACGTCACAGGCCATGCACCAGCTCTTCGGGCT
GTTTGTCACACTGATGTTTGCCTCTGTGGGCGGGGGCCTTGGAGGGCTCCTGCTGAAGCTACCCTTTCTG
GACTCCCCCCCAGACTCCCAGCACTACGAGGACCAAGTTCACTGGCAGGTGCCTGGCGAGCATGAGGATA
AAGCCCAGAGACCTCTGAGGGTGGAGGAGGCAGACACTCAGGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC217600 representing NM_020407
Red=Cloning site Green=Tags(s)

MAGSPSRAAGRRLQLPLLCLFLQGATAVLFAVFVRYNHKTDAALWHRSNHSNADNEFYFRYPSFQDVHAM
VFVGFGFLMVFLQRYGFSSVGFTFLLAAFALQWSTLVQGFLHSFHGGHIHVGVESMINADFCAGAVLISF
GAVLGKTGPTQLLLMALLEVVLFGINEFVLLHLLGVRDAGGSMTIHTFGAYFGLVLSRVLYRPQLEKSKH
RQGSVYHSDLFAMIGTIFLWIFWPSFNAALTALGAGQHRTALNTYYSLAASTLGTFALSALVGEDGRLDM
VHIQNAALAGGVVVGTSSEMMLTPFGALAAGFLAGTVSTLGYKFFTPILESKFKVQDTCGVHNLHGMPGV
LGALLGVLVAGLATHEAYGDGLESVFPLIAEGQRSATSQAMHQLFGLFVTLMFASVGGGLGGLLLKLPFL
DSPPDSQHYEDQVHWQVPGEHEDKAQRPLRVEEADTQA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020407
ORF Size 1374 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020407.5
RefSeq Size 1805 bp
RefSeq ORF 1377 bp
Locus ID 57127
UniProt ID Q9H310
Cytogenetics 1q22
Protein Families Transmembrane
MW 49.3 kDa
Summary This gene encodes one of two non-erythroid members of the Rhesus (Rh) protein family. Non-erythroid Rh protein family members are mainly expressed in the kidney and belong to the methylammonium-ammonium permease/ammonia transporters superfamily. All Rh family proteins are predicted to be transmembrane proteins with 12 membrane spanning domains and intracytoplasmic N- and C-termini. Alternative splicing of this gene results in multiple transcript variants. provided by RefSeq, Jan 2012
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Documents
Resources
"NM_020407" in other vectors (4)
SKU Description Size Price
RC217600L3 Lenti ORF clone of Human Rh family, B glycoprotein (gene/pseudogene) (RHBG), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC217600L4 Lenti ORF clone of Human Rh family, B glycoprotein (gene/pseudogene) (RHBG), transcript variant 1, mGFP tagged 10 ug
$757.00
RG217600 RHBG (tGFP-tagged) - Human Rh family, B glycoprotein (gene/pseudogene) (RHBG), transcript variant 1 10 ug
$657.00
SC304759 RHBG (untagged)-Human Rh family, B glycoprotein (gene/pseudogene) (RHBG), transcript variant 1 10 ug
$503.00

Plasmid Map for RC217600

Download sequence for

Cloning scheme for RC217600

Circular map for RC217600

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