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TdT (DNTT) (NM_004088) Human Tagged ORF Clone

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SKU
RC217586
DNTT (Myc-DDK-tagged)-Human deoxynucleotidyltransferase, terminal (DNTT), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $474.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TdT
Synonyms TDT
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC217586 representing NM_004088
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCCACCACGAGCGTCCCACTTGAGCCCTCGGAAGAAGAGACCCCGGCAGACGGGTGCCTTGATGG
CCTCCTCTCCTCAAGACATCAAATTTCAAGATTTGGTCGTCTTCATTTTGGAGAAGAAAATGGGAACCAC
CCGCAGAGCGTTCCTCATGGAGCTGGCCCGCAGGAAAGGGTTCAGGGTTGAAAATGAGCTCAGTGATTCT
GTCACCCACATTGTAGCAGAGAACAACTCGGGTTCGGATGTTCTGGAGTGGCTTCAAGCACAGAAAGTAC
AAGTCAGCTCACAACCAGAGCTCCTCGATGTCTCCTGGCTGATCGAATGCATAGGAGCAGGGAAACCGGT
GGAAATGACAGGAAAACACCAGCTTGTTGTGAGAAGAGACTATTCAGATAGCACCAACCCAGGCCCCCCG
AAGACTCCACCAATTGCTGTACAAAAGATCTCCCAGTATGCGTGTCAGAGAAGAACCACTTTAAACAACT
GTAACCAGATATTCACGGATGCCTTTGATATACTGGCTGAAAACTGTGAGTTTAGAGAAAATGAAGACTC
CTGTGTGACATTTATGAGAGCAGCTTCTGTATTGAAATCTCTGCCATTCACAATCATCAGTATGAAGGAC
ACAGAAGGAATTCCCTGCCTGGGGTCCAAGGTGAAGGGTATCATAGAGGAGATTATTGAAGATGGAGAAA
GTTCTGAAGTTAAAGCTGTGTTAAATGATGAACGATATCAATCCTTCAAACTCTTTACTTCTGTATTTGG
AGTGGGGCTGAAGACTTCTGAGAAGTGGTTCAGGATGGGTTTCAGAACTCTGAGTAAAGTAAGGTCGGAC
AAAAGCCTGAAATTTACACGAATGCAGAAAGCAGGATTTCTGTATTATGAAGACCTTGTCAGCTGTGTGA
CCAGGGCAGAAGCAGAGGCCGTCAGTGTGCTGGTTAAAGAGGCTGTCTGGGCATTTCTTCCGGATGCTTT
CGTCACCATGACAGGAGGGTTCCGGAGGGGTAAGAAGATGGGGCATGATGTAGATTTTTTAATTACCAGC
CCAGGATCAACAGAGGATGAAGAGCAACTTTTACAGAAAGTGATGAACTTATGGGAAAAGAAGGGATTAC
TTTTATATTATGACCTTGTGGAGTCAACATTTGAAAAGCTCAGGTTGCCTAGCAGGAAGGTTGATGCTTT
GGATCATTTTCAAAAGTGCTTTCTGATTTTCAAATTGCCTCGTCAAAGAGTGGACAGTGACCAGTCCAGC
TGGCAGGAAGGAAAGACCTGGAAGGCCATCCGTGTGGATTTAGTTCTGTGCCCCTACGAGCGTCGTGCCT
TTGCCCTGTTGGGATGGACTGGCTCCCGGCAGTTTGAGAGAGACCTCCGGCGCTATGCCACACATGAGCG
GAAGATGATTCTGGATAACCATGCTTTATATGACAAGACCAAGAGGATATTCCTCAAAGCAGAAAGTGAA
GAAGAAATTTTTGCGCATCTGGGATTGGATTATATTGAACCGTGGGAAAGAAATGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC217586 representing NM_004088
Red=Cloning site Green=Tags(s)

MDPPRASHLSPRKKRPRQTGALMASSPQDIKFQDLVVFILEKKMGTTRRAFLMELARRKGFRVENELSDS
VTHIVAENNSGSDVLEWLQAQKVQVSSQPELLDVSWLIECIGAGKPVEMTGKHQLVVRRDYSDSTNPGPP
KTPPIAVQKISQYACQRRTTLNNCNQIFTDAFDILAENCEFRENEDSCVTFMRAASVLKSLPFTIISMKD
TEGIPCLGSKVKGIIEEIIEDGESSEVKAVLNDERYQSFKLFTSVFGVGLKTSEKWFRMGFRTLSKVRSD
KSLKFTRMQKAGFLYYEDLVSCVTRAEAEAVSVLVKEAVWAFLPDAFVTMTGGFRRGKKMGHDVDFLITS
PGSTEDEEQLLQKVMNLWEKKGLLLYYDLVESTFEKLRLPSRKVDALDHFQKCFLIFKLPRQRVDSDQSS
WQEGKTWKAIRVDLVLCPYERRAFALLGWTGSRQFERDLRRYATHERKMILDNHALYDKTKRIFLKAESE
EEIFAHLGLDYIEPWERNA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004088
ORF Size 1527 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004088.2
RefSeq Size 2071 bp
RefSeq ORF 1530 bp
Locus ID 1791
UniProt ID P04053
Cytogenetics 10q24.1
Protein Pathways Hematopoietic cell lineage, Non-homologous end-joining
MW 58.4 kDa
Summary This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. provided by RefSeq, Jul 2008
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"NM_004088" in other vectors (6)
SKU Description Size Price
RC217586L1 Lenti ORF clone of Human deoxynucleotidyltransferase, terminal (DNTT), transcript variant 1, Myc-DDK-tagged 10 ug
$774.00
RC217586L2 Lenti ORF clone of Human deoxynucleotidyltransferase, terminal (DNTT), transcript variant 1, mGFP tagged 10 ug
$774.00
RC217586L3 Lenti ORF clone of Human deoxynucleotidyltransferase, terminal (DNTT), transcript variant 1, Myc-DDK-tagged 10 ug
$774.00
RC217586L4 Lenti ORF clone of Human deoxynucleotidyltransferase, terminal (DNTT), transcript variant 1, mGFP tagged 10 ug
$774.00
RG217586 DNTT (tGFP-tagged) - Human deoxynucleotidyltransferase, terminal (DNTT), transcript variant 1 10 ug
$489.00 $674.00
SC122668 DNTT (untagged)-Human deoxynucleotidyltransferase, terminal (DNTT), transcript variant 1 10 ug
$475.00

Plasmid Map for RC217586

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC217586
Circular map for RC217586

file-alt Citations

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