GPLD1 (NM_001503) Human Tagged ORF Clone

SKU
RC217578
GPLD1 (Myc-DDK-tagged)-Human glycosylphosphatidylinositol specific phospholipase D1 (GPLD1), transcript variant 1
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GPLD1
Synonyms GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; PLD
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC217578 representing NM_001503
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGCTTTCAGGTTGTGGCCTGGCCTGCTGATCATGTTGGGTTCTCTCTGCCATAGAGGTTCACCGT
GTGGCCTTTCAACACACGTAGAAATAGGACACAGAGCTCTGGAGTTTCTTCAGCTTCACAATGGGCGTGT
TAACTACAGAGAGCTGTTACTAGAACACCAGGATGCGTATCAGGCTGGAATCGTGTTTCCTGATTGTTTT
TACCCTAGCATCTGCAAAGGAGGAAAATTCCATGATGTGTCTGAGAGCACTCACTGGACTCCGTTTCTTA
ATGCAAGCGTTCATTATATCCGAGAGAACTATCCCCTTCCCTGGGAGAAGGACACAGAGAAACTGGTAGC
TTTCTTGTTTGGAATTACTTCTCACATGGCGGCAGATGTCAGCTGGCATAGTCTGGGCCTTGAACAAGGA
TTCCTTAGGACCATGGGAGCTATTGATTTTCACGGCTCCTATTCAGAGGCTCATTCGGCTGGTGATTTTG
GAGGAGATGTGTTGAGCCAGTTTGAATTTAATTTTAATTACCTTGCACGACGCTGGTATGTGCCAGTCAA
AGATCTACTGGGAATTTATGAGAAACTGTATGGTCGAAAAGTCATCACCGAAAATGTAATCGTTGATTGT
TCACATATCCAGTTCTTAGAAATGTATGGTGAGATGCTAGCTGTTTCCAAGTTATATCCCACTTACTCTA
CAAAGTCCCCGTTTTTGGTGGAACAATTCCAAGAGTATTTTCTTGGAGGACTGGATGATATGGCATTTTG
GTCCACTAATATTTACCATCTAACAAGCTTCATGTTGGAGAATGGGACCAGTGACTGCAACCTGCCTGAG
AACCCTCTGTTCATTGCATGTGGCGGCCAGCAAAACCACACCCAGGGCTCAAAAATGCAGAAAAATGATT
TTCACAGAAATTTGACTACATCCCTAACTGAAAGTGTTGACAGGAATATAAACTATACTGAAAGAGGAGT
GTTCTTTAGTGTAAATTCCTGGACCCCGGATTCCATGTCCTTTATCTACAAGGCTTTGGAAAGGAACATA
AGGACAATGTTCATAGGTGGCTCTCAGTTGTCACAAAAGCACGTCTCCAGCCCCTTAGCATCTTACTTCT
TGTCATTTCCTTATGCGAGGCTTGGCTGGGCAATGACCTCAGCTGACCTCAACCAGGATGGGCACGGTGA
CCTCGTGGTGGGCGCACCAGGCTACAGCCGCCCCGGCCACATCCACATCGGGCGCGTGTACCTCATCTAC
GGCAATGACCTGGGCCTGCCACCTGTTGACCTGGACCTGGACAAGGAGGCCCACAGGATCCTTGAAGGCT
TCCAGCCCTCAGGTCGGTTTGGCTCGGCCTTGGCTGTGTTGGACTTTAACGTGGACGGCGTGCCTGACCT
GGCCGTGGGAGCTCCCTCGGTGGGCTCCGAGCAGCTCACCTACAAAGGTGCCGTGTATGTCTACTTTGGT
TCCAAACAAGGAGGAATGTCTTCTTCCCCTAACATCACCATTTCTTGCCAGGACATCTACTGTAACTTGG
GCTGGACTCTCTTGGCTGCAGATGTGAATGGAGACAGTGAACCCGATCTGGTCATCGGCTCCCCTTTTGC
ACCAGGTGGAGGGAAGCAGAAGGGAATTGTGGCTGCGTTTTATTCTGGCCCCAGCCTGAGCGACAAAGAA
AAACTGAACGTGGAGGCAGCCAACTGGACGGTGAGAGGCGAGGAAGACTTCTCCTGGTTTGGATATTCCC
TTCACGGTGTCACTGTGGACAACAGAACCTTGCTGTTGGTTGGGAGCCCGACCTGGAAGAATGCCAGCAG
GCTGGGCCATTTGTTACACATCCGAGATGAGAAAAAGAGCCTTGGGAGGGTGTATGGCTACTTCCCACCA
AACGGCCAAAGCTGGTTTACCATTTCTGGAGACAAGGCAATGGGGAAACTGGGTACTTCCCTTTCCAGTG
GCCACGTACTGATGAATGGGACTCTGAAACAAGTGCTGCTGGTTGGAGCCCCTACGTACGATGACGTGTC
TAAGGTGGCATTCCTGACCGTGACCCTACACCAAGGCGGAGCCACTCGCATGTACGCACTCATATCTGAC
GCGCAGCCTCTGCTGCTCAGCACCTTCAGCGGAGACCGCCGCTTCTCCCGATTTGGTGGCGTTCTGCACT
TGAGTGACCTGGATGATGATGGCTTAGATGAAATCATCATGGCAGCCCCCCTGAGGATAGCAGATGTAAC
CTCTGGACTGATTGGGGGAGAAGACGGCCGAGTATATGTATATAATGGCAAAGAGACCACCCTTGGTGAC
ATGACTGGCAAATGCAAATCATGGATAACTCCATGTCCAGAAGAAAAGGCCCAATATGTATTGATTTCTC
CTGAAGCCAGCTCAAGGTTTGGGAGCTCCCTCATCACCGTGAGGTCCAAGGCAAAGAACCAAGTCGTCAT
TGCTGCTGGAAGGAGTTCTTTGGGAGCCCGACTCTCCGGGGCACTTCACGTCTATAGCCTTGGCTCAGAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC217578 representing NM_001503
Red=Cloning site Green=Tags(s)

MSAFRLWPGLLIMLGSLCHRGSPCGLSTHVEIGHRALEFLQLHNGRVNYRELLLEHQDAYQAGIVFPDCF
YPSICKGGKFHDVSESTHWTPFLNASVHYIRENYPLPWEKDTEKLVAFLFGITSHMAADVSWHSLGLEQG
FLRTMGAIDFHGSYSEAHSAGDFGGDVLSQFEFNFNYLARRWYVPVKDLLGIYEKLYGRKVITENVIVDC
SHIQFLEMYGEMLAVSKLYPTYSTKSPFLVEQFQEYFLGGLDDMAFWSTNIYHLTSFMLENGTSDCNLPE
NPLFIACGGQQNHTQGSKMQKNDFHRNLTTSLTESVDRNINYTERGVFFSVNSWTPDSMSFIYKALERNI
RTMFIGGSQLSQKHVSSPLASYFLSFPYARLGWAMTSADLNQDGHGDLVVGAPGYSRPGHIHIGRVYLIY
GNDLGLPPVDLDLDKEAHRILEGFQPSGRFGSALAVLDFNVDGVPDLAVGAPSVGSEQLTYKGAVYVYFG
SKQGGMSSSPNITISCQDIYCNLGWTLLAADVNGDSEPDLVIGSPFAPGGGKQKGIVAAFYSGPSLSDKE
KLNVEAANWTVRGEEDFSWFGYSLHGVTVDNRTLLLVGSPTWKNASRLGHLLHIRDEKKSLGRVYGYFPP
NGQSWFTISGDKAMGKLGTSLSSGHVLMNGTLKQVLLVGAPTYDDVSKVAFLTVTLHQGGATRMYALISD
AQPLLLSTFSGDRRFSRFGGVLHLSDLDDDGLDEIIMAAPLRIADVTSGLIGGEDGRVYVYNGKETTLGD
MTGKCKSWITPCPEEKAQYVLISPEASSRFGSSLITVRSKAKNQVVIAAGRSSLGARLSGALHVYSLGSD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_001503
ORF Size 2520 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001503.4
RefSeq Size 3489 bp
RefSeq ORF 2523 bp
Locus ID 2822
UniProt ID P80108
Cytogenetics 6p22.3
Protein Families Druggable Genome, Secreted Protein
Protein Pathways Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
MW 92.35 kDa
Summary Many proteins are tethered to the extracellular face of eukaryotic plasma membranes by a glycosylphosphatidylinositol (GPI) anchor. The GPI-anchor is a glycolipid found on many blood cells. The protein encoded by this gene is a GPI degrading enzyme. Glycosylphosphatidylinositol specific phospholipase D1 hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans, thereby releasing the attached protein from the plasma membrane. [provided by RefSeq, Jul 2008]
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Citations

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