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AJM1 (NM_001080482) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC217132
C9orf172 (Myc-DDK-tagged)-Human chromosome 9 open reading frame 172 (C9orf172)
ORF Plasmid:
DDK tGFP
$589.00 $894.00
20 Weeks*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol AJM1
Synonyms ajm-1; C9orf172
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC217132 representing NM_001080482
Red=Cloning site Blue=ORF Green=Tags(s)

CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCCGTACGGACCCTCCGGACCTGCTGGTGTCGACCGTGTACCAGGACATCAAGGTGGCGACCCCGG
GACCCGCGTCCAAGTGCTCGCCATGTGAGCGATCCGTGGCCCGGCCTGCTGAGCCCGCGCCTTTCAACAA
GCGCCACTGCCGCAGCTTCGACTTCCTGGAGGCGCTGGACGGGCCGGCCATGGAGACCCTGCCGGAGCCA
CCGCCGCCGGAGTCCGCTGTGCCGCGCGCCCGGACCCGCGAAGCCGAGCCACGCCGCCGCGCCCGCTCCA
AGAGTGCGCCCCGCGCGCCCCCGGGCCTGACGCCCGCGCCCGCCTCGCCGCCGGTGTTGCCCCGCCGAGG
GCGGGAGGCCCAGCGTGCGGCGCGGGCCGAGGCATCGCCGCGCCGGGAGCCCGCGTACCCGGCGCTCCGC
GCCCTTGCCAACGAGCTGCATCCCATCAAGTTGCAGCCGCAGCGGGGCGGCCCCGGCCGCGTCGCGCCCC
TGTGCGCCGCCGCGGGCCGCTGCGCACCGCCCGAGCCACCCGCGGGTCCCGCCCCCCACGTGCGCTGCCG
CCTGGACATCAAGCCAGACGACGCAGTGCTCCAGCACGCCACCCGGGGCTCGCGGTCCTGCGGGCCCACC
GAGGCCGCGCACTGGGCCCGCCCCGCCCCGCAGTTCCACGGCCTCACGGTGCCCGGGCCCCGCCACATGG
CGCTGTCGCGCACCCCGACGCCCAGCGACTCATACTGTGCGGATCCCCGGGCGTTCTACTGCGACGGGCC
CCTGCCTGGGCCCCGGGACTACGCCGAGCGCCGCAGTCTGCCCTTCACCACCCCGCCGGGCCCCACCCAG
TTCTTCTATACAGAGGAGCCCCAAGGCTTCCGGGGCAGCTTTGCAGCCAGTCCCGGCCCAACCTTCGACG
CCTACTACCCCAGGCCCTATCCGTCCGAGGAGCTCTCGGGGCCCAGTCCAAGGCGCATGGGCGGCTACTA
CGCAGGAGAGGTGCGCACCTTCCCAATCCAGGAACCGCCCTCCCGCTCCTACTATGGGGAGGCTCCACGA
GCCTACGGCCTGCCCTACGGGCCCCGCTATGTCCCCGAGGAGCCCCGGGCCCACTCCACCGCCCGCCCCT
TTTACACGGAGGACTTCGGAAGGTACCGCGAGCGTGACGTCCTGGCTCGGACGTACCCGCACCCGCGCAG
CAGCCCGGCCTGGGCGGACTGGGGCCCGCGACCGTACCGCACCCTGCAAGTGGTGCCGCCCTCTGACCCC
GACCCGTTGCTCGCCTCCTGGCACGGCGGCACCGGCACCAGTCCGCCCCGGCTGGCCACCGACAGCCGCC
ACTACTCGCGCTCCTGGGACAACATTCTGGCCCCGGGGCCGCGCCGAGAAGACCCGTTGGGCCGCGGCCG
CAGCTACGAGAACCTGCTGGGGCGCGAGGTGCGGGAGCCGCGAGGCGTGTCCCCCGAAGGCCGGCGCCCG
CCCGTCGTCGTGAACCTGTCCACCTCTCCCAGACGCTACGCCGCACTGTCCCTGTCCGAGACGTCGCTGA
CGGAGAAGGGCCGCGCGGGCGAGGGCCTGGGCCGCAACTGGTACGTGACGCCCGAGATCACCATCACTGA
CAATGACCTGCGCGCCACCGAGCGCCCGAGCGCCAGGGCCTGGGAGTTGCCCGGGGGCCGCACGCGGCCA
CCTCCCCACGCGGCCCCCGACGGCCCCACCTCTGGCCGCCAGCGGAGCCTAGAGCAGCTGGACGAGCTCA
TCACGGACCTGGTCATCGACTCGCGACCCACCGCCGGCCAGGCCTCAGAGCCCGCGGCCGACTGCCTGGG
CCCCCAACTGCGCCGACTGCTGGACTCGCGGCCCGCGGGCTCCGGGGCCCCCGCGCTGGCGCCGCCACGC
TCGCCCCCCGCCTCGGCCGGCAGCGCCGAGGAGCCCGCGGCCCCGGGAGAGGCGGCCGACGCGTCCCCCG
AACCCAGCGCCGACGAGGACGACCTGATGACCTGCTCCAATGCGCGCTGCCGGCGCACCGAGACCATGTT
CAACGCCTGCCTCTACTTCAAGTCCTGCCACAGCTGCTACACCTACTACTGCTCGCGCCTGTGCCGCCGC
GAGGACTGGGACGCCCACAAGGCGCGCTGCGTGTACGGCCGCGTGGGCAGCGTGTGCCGCCACGTACTGC
AGTTTTGCCGCGACAGCGGCCCGGTGCACCGCGCTTTCTCGCGCATCGCGCGTGTCGGCTTCCTGTCGCG
CGGCCGCGGCGTGCTCTTCCTGGGCTTCCCAAGTCCAGGCTCGGCCGACAACTTCCTGCGCTTTGGCCTG
GAGGGGCTGCTGCTATCCCCCACCTACCTATCGCTGCGTGAGCTGGCCACACACGCGGCGCCCCTGGGCA
GCTACGCGCGCGAGCTGGCGGCCGCTGGGCGCCTCTACGAACCGGCAGAGTGCTTCCTGCTCAGCGTGTC
CGTGGCCGTGGGACCCGGCACCGCGCCACCGGGGACACCGGCCCTGCCCGCGCCCGCGCCACGCAGCCAC
GGGCCAACAGTGCGCAAGTTCGCCAAGGTAGCGCTGGCGGCCGGCAGCCCCGCGCGGCCGCCCCCGGCGC
GGAGCCGCGAGCCCGACATGGAGACGCTGATCCTGACGCCACCGCCGGGCACGGCGGGCCTGGATCAGGA
CGGCGAGGCGGGCCGGCGCGCGCGCGAGGTGGCCTTCATCCACATCCAGCGCGAGCTGCGGCTGCGCGGC
GTCTTCCTGCGCCACGAGTTCCCGCGCGTCTACGAGCAGCTTTGCGAGTTCGTCGAGGCCAACAGGCGCT
TCACGCCCACCACCATCTACCCCACGGACCGGCGCACCGGCCGCCCCTTCATGTGCATGATCATGGCCGC
CTCCGAGCCGCGCGCGCTCGACTGGGTGGCCAGCGCCAACCTGCTGGACGACATCATG

CTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACG
ATAAGGTTTAA
Protein Sequence
>RC217132 representing NM_001080482
Red=Cloning site Green=Tags(s)

MTRTDPPDLLVSTVYQDIKVATPGPASKCSPCERSVARPAEPAPFNKRHCRSFDFLEALDGPAMETLPEP
PPPESAVPRARTREAEPRRRARSKSAPRAPPGLTPAPASPPVLPRRGREAQRAARAEASPRREPAYPALR
ALANELHPIKLQPQRGGPGRVAPLCAAAGRCAPPEPPAGPAPHVRCRLDIKPDDAVLQHATRGSRSCGPT
EAAHWARPAPQFHGLTVPGPRHMALSRTPTPSDSYCADPRAFYCDGPLPGPRDYAERRSLPFTTPPGPTQ
FFYTEEPQGFRGSFAASPGPTFDAYYPRPYPSEELSGPSPRRMGGYYAGEVRTFPIQEPPSRSYYGEAPR
AYGLPYGPRYVPEEPRAHSTARPFYTEDFGRYRERDVLARTYPHPRSSPAWADWGPRPYRTLQVVPPSDP
DPLLASWHGGTGTSPPRLATDSRHYSRSWDNILAPGPRREDPLGRGRSYENLLGREVREPRGVSPEGRRP
PVVVNLSTSPRRYAALSLSETSLTEKGRAGEGLGRNWYVTPEITITDNDLRATERPSARAWELPGGRTRP
PPHAAPDGPTSGRQRSLEQLDELITDLVIDSRPTAGQASEPAADCLGPQLRRLLDSRPAGSGAPALAPPR
SPPASAGSAEEPAAPGEAADASPEPSADEDDLMTCSNARCRRTETMFNACLYFKSCHSCYTYYCSRLCRR
EDWDAHKARCVYGRVGSVCRHVLQFCRDSGPVHRAFSRIARVGFLSRGRGVLFLGFPSPGSADNFLRFGL
EGLLLSPTYLSLRELATHAAPLGSYARELAAAGRLYEPAECFLLSVSVAVGPGTAPPGTPALPAPAPRSH
GPTVRKFAKVALAAGSPARPPPARSREPDMETLILTPPPGTAGLDQDGEAGRRAREVAFIHIQRELRLRG
VFLRHEFPRVYEQLCEFVEANRRFTPTTIYPTDRRTGRPFMCMIMAASEPRALDWVASANLLDDIM

LEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-XhoI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001080482
ORF Size 2928 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001080482.4
RefSeq Size 2931 bp
RefSeq ORF 2931 bp
Locus ID 389813
UniProt ID C9J069
Cytogenetics 9q34.3
MW 106.5 kDa
Summary May be involved in the control of adherens junction integrity.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001080482" in other vectors (2)
SKU Description Size Price
RG217132 C9orf172 (tGFP-tagged) - Human chromosome 9 open reading frame 172 (C9orf172) 10 ug
$1,094.00
SC318427 C9orf172 (untagged)-Human chromosome 9 open reading frame 172 (C9orf172) 10 ug
$984.00

Plasmid Map for RC217132

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC217132
Circular map for RC217132

file-alt Citations

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