GLIPR1 (NM_006851) Human Tagged ORF Clone

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SKU
RC216882
GLIPR1 (Myc-DDK-tagged)-Human GLI pathogenesis-related 1 (GLIPR1)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GLIPR1
Synonyms CRISP7; GLIPR; RTVP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC216882 representing NM_006851
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGTGTCACACTTGCTACAATAGCCTGGATGGTTTCTTTTGTCTCCAATTATTCACACACAGCAAATA
TTTTGCCAGATATCGAAAATGAAGATTTCATCAAAGACTGCGTTCGAATCCATAACAAGTTCCGATCAGA
GGTGAAACCAACAGCCAGTGATATGCTATACATGACTTGGGACCCAGCACTAGCCCAAATTGCAAAAGCA
TGGGCCAGCAATTGCCAGTTTTCACATAATACACGGCTGAAGCCACCCCACAAGCTGCACCCAAACTTCA
CTTCACTGGGAGAGAACATCTGGACTGGGTCTGTGCCCATTTTTTCTGTGTCTTCCGCCATCACAAACTG
GTATGACGAAATCCAGGACTATGACTTCAAGACTCGGATATGCAAAAAAGTCTGTGGCCACTACACTCAG
GTTGTTTGGGCAGATAGTTACAAAGTTGGCTGCGCAGTTCAATTTTGCCCTAAAGTTTCTGGCTTTGACG
CTCTTTCCAATGGAGCACATTTTATATGCAACTACGGACCAGGAGGGAATTACCCAACTTGGCCATATAA
GAGAGGAGCCACCTGCAGTGCCTGCCCCAATAATGACAAGTGTTTGGACAATCTCTGTGTTAACCGACAG
CGAGACCAAGTCAAACGTTACTACTCTGTTGTATATCCAGGCTGGCCCATATATCCACGTAACAGATACA
CTTCTCTCTTTCTCATTGTTAATTCAGTAATTCTAATACTGTCTGTTATAATTACCATTTTGGTACAGCA
CAAGTACCCTAATTTAGTTCTTTTGGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC216882 representing NM_006851
Red=Cloning site Green=Tags(s)

MRVTLATIAWMVSFVSNYSHTANILPDIENEDFIKDCVRIHNKFRSEVKPTASDMLYMTWDPALAQIAKA
WASNCQFSHNTRLKPPHKLHPNFTSLGENIWTGSVPIFSVSSAITNWYDEIQDYDFKTRICKKVCGHYTQ
VVWADSYKVGCAVQFCPKVSGFDALSNGAHFICNYGPGGNYPTWPYKRGATCSACPNNDKCLDNLCVNRQ
RDQVKRYYSVVYPGWPIYPRNRYTSLFLIVNSVILILSVIITILVQHKYPNLVLLD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006851
ORF Size 798 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006851.3
RefSeq Size 1061 bp
RefSeq ORF 801 bp
Locus ID 11010
UniProt ID P48060
Cytogenetics 12q21.2
Domains SCP
Protein Families Transmembrane
MW 30.37 kDa
Summary This gene encodes a protein with similarity to both the pathogenesis-related protein (PR) superfamily and the cysteine-rich secretory protein (CRISP) family. Increased expression of this gene is associated with myelomocytic differentiation in macrophage and decreased expression of this gene through gene methylation is associated with prostate cancer. The protein has proapoptotic activities in prostate and bladder cancer cells. This gene is a member of a cluster on chromosome 12 containing two other similar genes. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_006851" in other vectors (6)
SKU Description Size Price
RC216882L1 Lenti ORF clone of Human GLI pathogenesis-related 1 (GLIPR1), Myc-DDK-tagged 10 ug
$600.00
RC216882L2 Lenti ORF clone of Human GLI pathogenesis-related 1 (GLIPR1), mGFP tagged 10 ug
$600.00
RC216882L3 Lenti ORF clone of Human GLI pathogenesis-related 1 (GLIPR1), Myc-DDK-tagged 10 ug
$600.00
RC216882L4 Lenti ORF clone of Human GLI pathogenesis-related 1 (GLIPR1), mGFP tagged 10 ug
$600.00
RG216882 GLIPR1 (tGFP-tagged) - Human GLI pathogenesis-related 1 (GLIPR1) 10 ug
$489.00 $500.00
SC108517 GLIPR1 (untagged)-Human GLI pathogenesis-related 1 (GLIPR1) 10 ug
$300.00

Plasmid Map for RC216882

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Cloning scheme for RC216882

Circular map for RC216882

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