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ARHGEF4 (NM_032995) Human Tagged ORF Clone

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SKU
RC216845
ARHGEF4 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 4 (ARHGEF4), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $614.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ARHGEF4
Synonyms ASEF; ASEF1; GEF4; STM6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC216845 representing NM_032995
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCTGGGAAGAACCAGCAGGTGAGAAGCCCAGTTGCTCTCACAGTCAGAAGGCGTTCCACATGGAGC
CTGCCCAGAAGCCCTGCTTCACCACTGACATGGTGACATGGGCCCTCCTCTGCATCTCTGCAGAGACTGT
GCGTGGGGAGGCTCCTTCACAGCCTAGGGGCATCCCTCACCGCTCGCCCGTCAGTGTGGATGACCTGTGG
CTGGAGAAGACACAGAGAAAGAAGTTGCAGAAGCAGGCCCACGTCGAAAGGAGGCTGCACATAGGGGCAG
TGCACAAAGATGGAGTCAAGTGCTGGAGAAAGACGATCATTACCTCTCCAGAGTCTTTGAATCTCCCTAG
AAGAAGCCATCCACTCTCCCAGAGTGCTCCAACGGGACTGAACCACATGGGCTGGCCAGAGCACACACCA
GGCACTGCCATGCCTGATGGAGCTCTGGACACAGCTGTCTGCGCTGACGAAGTGGGGAGCGAGGAGGACC
TGTATGATGACCTGCACAGCTCCAGCCACCACTACAGCCACCCTGGAGGGGGTGGGGAGCAGCTGGCTAT
CAATGAGCTCATCAGCGATGGCAGTGTGGTCTGCGCTGAAGCACTCTGGGACCATGTCACCATGGACGAC
CAGGAGCTGGGCTTCAAAGCTGGGGACGTCATCGAAGTGATGGATGCCACCAACAGAGAGTGGTGGTGGG
GCCGGGTCGCCGATGGCGAGGGCTGGTTTCCAGCCAGCTTCGTTCGGCTGAGGGTGAATCAGGACGAGCC
CGCGGATGACGACGCCCCTCTGGCCGGGAACAGCGGAGCGGAGGACGGCGGGGCGGAGGCGCAGAGCAGC
AAGGACCAGATGCGGACCAACGTCATCAACGAGATCCTCAGCACTGAGCGGGACTACATCAAGCACCTGC
GCGACATCTGCGAGGGCTACGTCCGGCAGTGCCGCAAGCGCGCAGACATGTTCAGCGAGGAGCAGCTGCG
TACCATCTTCGGGAACATCGAGGACATCTACCGCTGCCAGAAGGCCTTCGTGAAGGCCCTGGAGCAGAGG
TTCAACCGCGAGCGCCCACACCTGAGCGAGCTGGGTGCCTGCTTCCTGGAGCATCAAGCCGACTTCCAGA
TCTACTCGGAGTACTGCAATAACCACCCCAACGCCTGCGTGGAGCTCTCCCGGCTCACCAAGCTCAGCAA
GTACGTGTACTTCTTCGAGGCCTGCCGGCTGCTGCAGAAGATGATTGACATCTCCCTGGATGGCTTCCTG
CTGACTCCGGTGCAGAAGATCTGCAAGTACCCTCTGCAGCTGGCCGAGCTGCTCAAATACACGCACCCCC
AGCACAGGGACTTCAAGGATGTTGAAGCCGCCTTGCATGCCATGAAGAACGTGGCCCAGCTCATCAACGA
GCGGAAGCGGAGACTTGAGAACATCGACAAGATTGCTCAGTGGCAGAGCTCCATAGAGGACTGGGAGGGA
GAAGATCTCTTGGTCAGGAGCTCAGAACTCATCTACTCGGGGGAGCTGACTCGAGTTACACAGCCTCAAG
CCAAAAGCCAGCAGCGAATGTTCTTTCTCTTTGACCACCAGCTCATCTACTGTAAGAAGGACCTGCTCCG
CCGCGACGTGTTGTACTACAAGGGCCGGCTGGACATGGACGGCCTGGAGGTGGTGGACCTGGAGGACGGG
AAGGACAGAGACCTCCATGTGAGCATCAAGAACGCCTTCCGGCTGCACCGTGGCGCCACAGGGGACAGCC
ACCTGCTGTGCACCAGGAAGCCCGAGCAGAAGCAGCGCTGGCTCAAGGCCTTTGCCAGGGAGAGGGAGCA
GGTGCAGCTGGACCAGGAGACAGGCTTCTCCATCACTGAACTGCAGAGGAAGCAGGCCATGCTGAATGCC
AGCAAGCAGCAGGTCACAGGGAAGCCCAAAGGTAGGCGGACAGCAGCCCCACCTCCTCGGCTGCCCGGTC
CTTACCCTGCTGACATCATTCCCTTCTCTGAACCACAAAGCCAAGCCAGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC216845 representing NM_032995
Red=Cloning site Green=Tags(s)

MPWEEPAGEKPSCSHSQKAFHMEPAQKPCFTTDMVTWALLCISAETVRGEAPSQPRGIPHRSPVSVDDLW
LEKTQRKKLQKQAHVERRLHIGAVHKDGVKCWRKTIITSPESLNLPRRSHPLSQSAPTGLNHMGWPEHTP
GTAMPDGALDTAVCADEVGSEEDLYDDLHSSSHHYSHPGGGGEQLAINELISDGSVVCAEALWDHVTMDD
QELGFKAGDVIEVMDATNREWWWGRVADGEGWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSS
KDQMRTNVINEILSTERDYIKHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQR
FNRERPHLSELGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFL
LTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQWQSSIEDWEG
EDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDVLYYKGRLDMDGLEVVDLEDG
KDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKAFAREREQVQLDQETGFSITELQRKQAMLNA
SKQQVTGKPKGRRTAAPPPRLPGPYPADIIPFSEPQSQAS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032995
ORF Size 2010 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032995.2, NP_127462.1
RefSeq Size 4258 bp
RefSeq ORF 2013 bp
Locus ID 50649
UniProt ID Q9NR80, Q9NTG0
Cytogenetics 2q21.1
Protein Families Druggable Genome
Protein Pathways Regulation of actin cytoskeleton
MW 76.2 kDa
Summary Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The protein encoded by this gene may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of some variants has not been determined. provided by RefSeq, Jun 2013
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"NM_032995" in other vectors (4)
SKU Description Size Price
RC216845L3 Lenti-ORF clone of ARHGEF4 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 4 (ARHGEF4), transcript variant 2 10 ug
$914.00
RC216845L4 Lenti-ORF clone of ARHGEF4 (mGFP-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 4 (ARHGEF4), transcript variant 2 10 ug
$914.00
RG216845 ARHGEF4 (tGFP-tagged) - Human Rho guanine nucleotide exchange factor (GEF) 4 (ARHGEF4), transcript variant 2 10 ug
$814.00
SC305530 ARHGEF4 (untagged)-Human Rho guanine nucleotide exchange factor (GEF) 4 (ARHGEF4), transcript variant 2 10 ug
$676.00

Plasmid Map for RC216845

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC216845
Circular map for RC216845

file-alt Citations

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