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PAX7 (NM_013945) Human Tagged ORF Clone

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SKU
RC216836
PAX7 (Myc-DDK-tagged)-Human paired box 7 (PAX7), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $482.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PAX7
Synonyms HUP1; MYOSCO; PAX7B; RMS2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC216836 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCCCTTCCCGGCACGGTACCGAGAATGATGCGGCCGGCTCCGGGGCAGAACTACCCCCGCACGG
GATTCCCTTTGGAAGTGTCCACCCCGCTTGGCCAAGGCCGGGTCAATCAGCTGGGAGGGGTCTTCATCAA
TGGGCGACCCCTGCCTAACCACATCCGCCACAAGATAGTGGAGATGGCCCACCATGGCATCCGGCCCTGT
GTCATCTCCCGACAGCTGCGTGTCTCCCACGGCTGCGTCTCCAAGATTCTTTGCCGCTACCAGGAGACCG
GGTCCATCCGGCCTGGGGCCATCGGCGGCAGCAAGCCCAGACAGGTGGCGACTCCGGATGTAGAGAAAAA
GATTGAGGAGTACAAGAGGGAAAACCCAGGCATGTTCAGCTGGGAGATCCGGGACAGGCTGCTGAAGGAT
GGGCACTGTGACCGAAGCACTGTGCCCTCAGTGAGTTCGATTAGCCGCGTGCTCAGAATCAAGTTCGGGA
AGAAAGAGGAGGAGGATGAAGCGGACAAGAAGGAGGACGACGGCGAAAAGAAGGCCAAACACAGCATCGA
CGGCATCCTGGGCGACAAAGGGAACCGGCTGGACGAGGGCTCGGATGTGGAGTCGGAACCTGACCTCCCA
CTGAAGCGCAAGCAGCGACGCAGTCGGACCACATTCACGGCCGAGCAGCTGGAGGAGCTGGAGAAGGCCT
TTGAGAGGACCCACTACCCAGACATATACACCCGCGAGGAGCTGGCGCAGAGGACCAAGCTGACAGAGGC
GCGTGTGCAGGTCTGGTTCAGTAACCGCCGCGCCCGTTGGCGTAAGCAGGCAGGAGCCAACCAGCTGGCG
GCGTTCAACCACCTTCTGCCAGGAGGCTTCCCGCCCACCGGCATGCCCACGCTGCCCCCCTACCAGCTGC
CGGACTCCACCTACCCCACCACCACCATCTCCCAAGATGGGGGCAGCACTGTGCACCGGCCTCAGCCCCT
GCCACCGTCCACCATGCACCAGGGCGGGCTGGCTGCAGCGGCTGCAGCCGCCGACACCAGCTCTGCCTAC
GGAGCCCGCCACAGCTTCTCCAGCTACTCTGACAGCTTCATGAATCCGGCGGCGCCCTCCAACCACATGA
ACCCGGTCAGCAACGGCCTGTCTCCTCAGGTGATGAGCATCTTGGGCAACCCCAGTGCGGTGCCCCCGCA
GCCACAGGCTGACTTCTCCATCTCCCCGCTGCATGGCGGCCTGGACTCGGCCACCTCCATCTCAGCCAGC
TGCAGCCAGCGGGCCGACTCCATCAAGCCAGGAGACAGCCTGCCCACCTCCCAGGCCTACTGCCCACCCA
CCTACAGCACCACCGGCTACAGCGTGGACCCCGTGGCCGGCTATCAGTACGGCCAGTACGGCCAGAGTGA
GTGCCTGGTGCCCTGGGCGTCCCCCGTCCCCATTCCTTCTCCCACCCCCAGGGCCTCCTGCTTGTTTATG
GAGAGCTACAAGGTGGTGTCAGGGTGGGGAATGTCCATTTCACAGATGGAAAAATTGAAGTCCAGCCAGA
TGGAACAGTTCACC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC216836 protein sequence
Red=Cloning site Green=Tags(s)

MAALPGTVPRMMRPAPGQNYPRTGFPLEVSTPLGQGRVNQLGGVFINGRPLPNHIRHKIVEMAHHGIRPC
VISRQLRVSHGCVSKILCRYQETGSIRPGAIGGSKPRQVATPDVEKKIEEYKRENPGMFSWEIRDRLLKD
GHCDRSTVPSVSSISRVLRIKFGKKEEEDEADKKEDDGEKKAKHSIDGILGDKGNRLDEGSDVESEPDLP
LKRKQRRSRTTFTAEQLEELEKAFERTHYPDIYTREELAQRTKLTEARVQVWFSNRRARWRKQAGANQLA
AFNHLLPGGFPPTGMPTLPPYQLPDSTYPTTTISQDGGSTVHRPQPLPPSTMHQGGLAAAAAAADTSSAY
GARHSFSSYSDSFMNPAAPSNHMNPVSNGLSPQVMSILGNPSAVPPQPQADFSISPLHGGLDSATSISAS
CSQRADSIKPGDSLPTSQAYCPPTYSTTGYSVDPVAGYQYGQYGQSECLVPWASPVPIPSPTPRASCLFM
ESYKVVSGWGMSISQMEKLKSSQMEQFT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013945
ORF Size 1554 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013945.3
RefSeq Size 2265 bp
RefSeq ORF 1557 bp
Locus ID 5081
UniProt ID P23759, Q2PJS5
Cytogenetics 1p36.13
Protein Families Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transcription Factors
MW 56.7 kDa
Summary This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Three transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Sep 2008
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Documents
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"NM_013945" in other vectors (6)
SKU Description Size Price
RC216836L1 Lenti ORF clone of Human paired box 7 (PAX7), transcript variant 2, Myc-DDK-tagged 10 ug
$782.00
RC216836L2 Lenti ORF clone of Human paired box 7 (PAX7), transcript variant 2, mGFP tagged 10 ug
$782.00
RC216836L3 Lenti ORF clone of Human paired box 7 (PAX7), transcript variant 2, Myc-DDK-tagged 10 ug
$782.00
RC216836L4 Lenti ORF clone of Human paired box 7 (PAX7), transcript variant 2, mGFP tagged 10 ug
$782.00
RG216836 PAX7 (tGFP-tagged) - Human paired box 7 (PAX7), transcript variant 2 10 ug
$489.00 $682.00
SC304018 PAX7 (untagged)-Human paired box 7 (PAX7), transcript variant 2 10 ug
$483.00

Plasmid Map for RC216836

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC216836
Circular map for RC216836

file-alt Citations

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