SDHAF1 (NM_001042631) Human Tagged ORF Clone
- TrueORF®
SKU
RC216831
SDHAF1 (Myc-DDK-tagged)-Human succinate dehydrogenase complex assembly factor 1 (SDHAF1), nuclear gene encoding mitochondrial protein
$225.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | SDHAF1 |
| Synonyms | LYRM8; MC2DN2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001042631 |
| ORF Size | 345 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001042631.2 |
| RefSeq Size | 1131 bp |
| RefSeq ORF | 348 bp |
| Locus ID | 644096 |
| UniProt ID | A6NFY7 |
| Cytogenetics | 19q13.12 |
| MW | 12.6 kDa |
| Summary | The succinate dehydrogenase (SDH) complex (or complex II) of the mitochondrial respiratory chain is composed of 4 individual subunits. The protein encoded by this gene resides in the mitochondria, and is essential for SDH assembly, but does not physically associate with the complex in vivo. Mutations in this gene are associated with SDH-defective infantile leukoencephalopathy (mitochondrial complex II deficiency).provided by RefSeq, Mar 2010 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001042631" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC216831L1 | Lenti ORF clone of Human succinate dehydrogenase complex assembly factor 1 (SDHAF1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged | 10 ug |
$525.00
|
|
| RC216831L2 | Lenti ORF clone of Human succinate dehydrogenase complex assembly factor 1 (SDHAF1), nuclear gene encoding mitochondrial protein, mGFP tagged | 10 ug |
$525.00
|
|
| RC216831L3 | Lenti ORF clone of Human succinate dehydrogenase complex assembly factor 1 (SDHAF1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged | 10 ug |
$525.00
|
|
| RC216831L4 | Lenti ORF clone of Human succinate dehydrogenase complex assembly factor 1 (SDHAF1), nuclear gene encoding mitochondrial protein, mGFP tagged | 10 ug |
$525.00
|
|
| RG216831 | SDHAF1 (tGFP-tagged) - Human succinate dehydrogenase complex assembly factor 1 (SDHAF1), nuclear gene encoding mitochondrial protein | 10 ug |
$425.00
|
|
| SC311341 | SDHAF1 (untagged)-Human succinate dehydrogenase complex assembly factor 1 (SDHAF1), nuclear gene encoding mitochondrial protein | 10 ug |
$225.00
|
|
Citations
Adiponectin enhances the bioenergetics of cardiac myocytes via an AMPK- and succinate dehydrogenase-dependent mechanism
Jeon, He, Austin et al
Cell Signal (2021) 78, 109866 DOI: 10.1016/j.cellsig.2020.109866
Jeon, He, Austin et al
Cell Signal (2021) 78, 109866 DOI: 10.1016/j.cellsig.2020.109866
Cited by 19
AI Summary: In this study, OriGene products including the AMPKγ1 shRNA (OriGene, #TL501527) and Sdhaf1 shRNA (OriGene, #TL711337) were used to knock down gene expression and demonstrate that the enhancement of cardiac myocyte bioenergetics by adiponectin depends on AMPK and SDH complex assembly.
Showing 1-1 of 1 papers.
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