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FLYWCH1 (NM_020912) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC216822
FLYWCH1 (Myc-DDK-tagged)-Human FLYWCH-type zinc finger 1 (FLYWCH1), transcript variant 2. Note: ORF is codon optimized
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $644.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FLYWCH1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC216822 ORF sequence, codon optimized.
Due to the complexity of NM_020912, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.

Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCCTGCCCGAACCAAGCGAACAGGAAGGAGAGTCTGTCAAAGCCGGCCAAGAGCCTAGCCCCAAAC
CCGGAACAGATGTGATCCCCGCCGCCCCTCGGAAACCAAGGGAGTTCTCTAAGCTCGTCCTCCTTACGGC
CTCAGACCAAGATGAGGACGGAGTGGGATCCAAGCCGCAGGAGGTGCACTGTGTGCTGTCACTCGAAATG
GCGGGGCCCGCCACCTTGGCCAGTACACTTCAGATACTCCCCGTCGAAGAGCAGGGAGGGGTGGTACAAC
CCGCCCTCGAGATGCCTGAACAAAAGTGCAGTAAACTGGACGCCGCTCCCCAGTCACTCGAATTCCTTCG
CACACCGTTCGGTGGGAGACTTCTCGTCCTTGAAAGTTTCTTGTATAAGCAGGAAAAGGCTGTAGGGGAC
AAGGTCTACTGGAAATGTAGACAGCACGCAGAACTCGGTTGTCGCGGCCGGGCTATCACCCGGGGCCTGA
GAGCCACAGTGATGAGAGGACATTGTCACGCCCCGGACGAACAGGGTCTGGAGGCTAGGAGGCAGCGCGA
GAAGCTGCCTAGCTTGGCACTGCCTGAGGGGCTGGGGGAGCCACAGGGACCTGAAGGCCCAGGGGGCAGA
GTCGAGGAACCACTGGAAGGCGTAGGACCTTGGCAGTGCCCGGAAGAACCCGAACCGACTCCGGGCCTGG
TTCTCAGTAAGCCTGCCCTGGAAGAGGAGGAAGCACCCCGCGCCCTGTCTCTGCTGTCCCTGCCCCCGAA
GAAGCGGAGCATTTTGGGCCTCGGACAAGCACGCCCCCTGGAGTTCCTCCGCACGTGCTATGGAGGAAGC
TTTCTCGTTCACGAAAGCTTCTTGTACAAAAGGGAAAAGGCCGTGGGCGATAAAGTGTATTGGACATGCC
GCGACCACGCCCTGCACGGGTGTCGGTCTAGAGCCATTACACAAGGCCAGAGGGTCACCGTAATGAGAGG
CCACTGCCACCAGCCAGACATGGAGGGCCTGGAGGCGCGACGGCAGCAAGAGAAAGCCGTAGAAACACTG
CAGGCAGGCCAGGACGGCCCAGGCAGCCAGGTTGACACACTGCTCCGCGGCGTGGATAGCTTGCTGTACA
GAAGAGGACCAGGGCCCCTCACACTGACAAGACCTCGCCCCAGAAAACGAGCTAAAGTTGAGGACCAAGA
GTTGCCGACACAGCCAGAGGCACCCGACGAACACCAGGATATGGACGCGGACCCTGGCGGACCCGAGTTT
CTTAAGACGCCGTTGGGCGGCAGCTTCCTTGTCTATGAGTCTTTCCTCTATCGGCGCGAGAAGGCAGCCG
GCGAAAAGGTGTACTGGACCTGTCGCGACCAGGCCAGAATGGGTTGCAGGTCCAGGGCCATCACTCAGGG
ACGGCGAGTGACAGTTATGCGCGGACACTGCCACCCACCTGACCTGGGAGGGCTGGAAGCACTGAGACAA
CGGGAGAAGCGCCCCAATACCGCGCAAAGGGGATCACCCGGGGGGCCAGAGTTCCTCAAGACGCCCCTGG
GAGGCTCATTCCTGGTCTATGAATCATTCCTTTATAGACGCGAGAAGGCCGCTGGGGAGAAGGTCTATTG
GACTTGCAGAGATCAGGCTCGAATGGGCTGTCGCTCTCGGGCCATCACTCAGGGCCGCCGCGTTATGGTT
ATGCGCAGGCATTGTCACCCCCCCGACTTGGGCGGACTCGAGGCCCTGCGACAGAGGGAGCACTTCCCAA
ACTTGGCACAGTGGGATTCCCCGGATCCCTTGAGGCCCTTGGAATTCCTGCGCACCAGCCTTGGGGGCCG
CTTCCTGGTGCATGAATCCTTCCTGTATCGCAAAGAGAAAGCTGCCGGCGAAAAAGTCTATTGGATGTGT
CGGGATCAGGCCAGACTGGGGTGTCGGAGTCGCGCCATAACCCAGGGCCACAGGATCATGGTAATGCGAT
CTCATTGTCACCAGCCCGACCTGGCTGGGTTGGAAGCCCTGAGACAGCGGGAAAGGCTTCCGACAACAGC
ACAACAAGAAGATCCAGAAAAAATCCAGGTGCAACTGTGCTTTAAAACCTGCAGTCCCGAAAGCCAGCAG
ATCTATGGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC216822 representing NM_020912
Red=Cloning site Green=Tags(s)

MPLPEPSEQEGESVKAGQEPSPKPGTDVIPAAPRKPREFSKLVLLTASDQDEDGVGSKPQEVHCVLSLEM
AGPATLASTLQILPVEEQGGVVQPALEMPEQKCSKLDAAPQSLEFLRTPFGGRLLVLESFLYKQEKAVGD
KVYWKCRQHAELGCRGRAITRGLRATVMRGHCHAPDEQGLEARRQREKLPSLALPEGLGEPQGPEGPGGR
VEEPLEGVGPWQCPEEPEPTPGLVLSKPALEEEEAPRALSLLSLPPKKRSILGLGQARPLEFLRTCYGGS
FLVHESFLYKREKAVGDKVYWTCRDHALHGCRSRAITQGQRVTVMRGHCHQPDMEGLEARRQQEKAVETL
QAGQDGPGSQVDTLLRGVDSLLYRRGPGPLTLTRPRPRKRAKVEDQELPTQPEAPDEHQDMDADPGGPEF
LKTPLGGSFLVYESFLYRREKAAGEKVYWTCRDQARMGCRSRAITQGRRVTVMRGHCHPPDLGGLEALRQ
REKRPNTAQRGSPGGPEFLKTPLGGSFLVYESFLYRREKAAGEKVYWTCRDQARMGCRSRAITQGRRVMV
MRRHCHPPDLGGLEALRQREHFPNLAQWDSPDPLRPLEFLRTSLGGRFLVHESFLYRKEKAAGEKVYWMC
RDQARLGCRSRAITQGHRIMVMRSHCHQPDLAGLEALRQRERLPTTAQQEDPEKIQVQLCFKTCSPESQQ
IYG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020912
ORF Size 2109 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020912.1, NP_065963.1
RefSeq Size 2555 bp
RefSeq ORF 2112 bp
Locus ID 84256
UniProt ID Q4VC44
Cytogenetics 16p13.3
MW 78.7 kDa
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"NM_020912" in other vectors (4)
SKU Description Size Price
RC216822L3 Lenti-ORF clone of FLYWCH1 (Myc-DDK-tagged)-Human FLYWCH-type zinc finger 1 (FLYWCH1), transcript variant 2. Note: ORF is codon optimized 10 ug
$944.00
RC216822L4 Lenti-ORF clone of FLYWCH1 (mGFP-tagged)-Human FLYWCH-type zinc finger 1 (FLYWCH1), transcript variant 2. Note: ORF is codon optimized 10 ug
$944.00
RG216822 FLYWCH1 (tGFP-tagged) - Human FLYWCH-type zinc finger 1 (FLYWCH1), transcript variant 2. Note: ORF is codon optimized 10 ug
$844.00
SC304863 FLYWCH1 (untagged)-Human FLYWCH-type zinc finger 1 (FLYWCH1), transcript variant 2 10 ug
$709.00

Plasmid Map for RC216822

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC216822
Circular map for RC216822

file-alt Citations

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