MSI2 (NM_170721) Human Tagged ORF Clone
SKU
RC216799
MSI2 (Myc-DDK-tagged)-Human musashi homolog 2 (Drosophila) (MSI2), transcript variant 2
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | MSI2 |
| Synonyms | MSI2H |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-RsrII Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_170721 |
| ORF Size | 753 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_170721.2 |
| RefSeq Size | 2151 bp |
| RefSeq ORF | 756 bp |
| Locus ID | 124540 |
| UniProt ID | Q96DH6 |
| Cytogenetics | 17q22 |
| MW | 28.2 kDa |
| Summary | This gene encodes an RNA-binding protein that is a member of the Musashi protein family. The encoded protein is transcriptional regulator that targets genes involved in development and cell cycle regulation. Mutations in this gene are associated with poor prognosis in certain types of cancers. This gene has also been shown to be rearranged in certain cancer cells. provided by RefSeq, Apr 2016 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_170721" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC216799L1 | Lenti ORF clone of Human musashi homolog 2 (Drosophila) (MSI2), transcript variant 2, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC216799L2 | Lenti ORF clone of Human musashi homolog 2 (Drosophila) (MSI2), transcript variant 2, mGFP tagged | 10 ug |
$750.00
|
|
| RC216799L3 | Lenti ORF clone of Human musashi homolog 2 (Drosophila) (MSI2), transcript variant 2, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC216799L4 | Lenti ORF clone of Human musashi homolog 2 (Drosophila) (MSI2), transcript variant 2, mGFP tagged | 10 ug |
$750.00
|
|
| RG216799 | MSI2 (tGFP-tagged) - Human musashi homolog 2 (Drosophila) (MSI2), transcript variant 2 | 10 ug |
$650.00
|
|
| SC306686 | MSI2 (untagged)-Human musashi homolog 2 (Drosophila) (MSI2), transcript variant 2 | 10 ug |
$450.00
|
|
Citations
Evasion of regulatory phosphorylation by an alternatively spliced isoform of Musashi2
MacNicol, Cragle, McDaniel et al
Sci Rep (2017) 7 (1), 11503 DOI: 10.1038/s41598-017-11917-3
MacNicol, Cragle, McDaniel et al
Sci Rep (2017) 7 (1), 11503 DOI: 10.1038/s41598-017-11917-3
AI Summary: OriGene’s Human Msi2 variant 2 cDNA clone (Accession NM_170721) was used to generate expression constructs for functional analysis of the alternatively spliced Musashi2 isoform in this study.
Snippet: pXen hMsi2v2. Human Msi2 variant 2 (Accession NM_170721, Origene) was PCR amplified to include a 5′ Cla1 site, and a 3′ Xho1 site and cloned into Cla1/Xho1 digested pXen2
Showing 1-1 of 1 papers.
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in shipping.
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