KIF5B (NM_004521) Human Tagged ORF Clone
$1,322.00
2 Weeks*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | KIF5B |
| Synonyms | HEL-S-61; KINH; KNS; KNS1; UKHC |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_004521 |
| ORF Size | 2889 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004521.3 |
| RefSeq Size | 3688 bp |
| RefSeq ORF | 2892 bp |
| Locus ID | 3799 |
| UniProt ID | P33176 |
| Cytogenetics | 10p11.22 |
| Domains | kinesin |
| Protein Families | Druggable Genome |
| MW | 109.5 kDa |
| Summary | Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner (By similarity). Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes (PubMed:20386726). Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004521" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC216778L1 | Lenti-ORF clone of KIF5B (Myc-DDK-tagged)-Human kinesin family member 5B (KIF5B) | 10 ug |
$1,622.00
|
|
| RC216778L2 | Lenti-ORF clone of KIF5B (mGFP-tagged)-Human kinesin family member 5B (KIF5B) | 10 ug |
$1,622.00
|
|
| RC216778L3 | Lenti-ORF clone of KIF5B (Myc-DDK-tagged)-Human kinesin family member 5B (KIF5B) | 10 ug |
$1,622.00
|
|
| RC216778L4 | Lenti-ORF clone of KIF5B (mGFP-tagged)-Human kinesin family member 5B (KIF5B) | 10 ug |
$1,622.00
|
|
| RG216778 | KIF5B (tGFP-tagged) - Human kinesin family member 5B (KIF5B) | 10 ug |
$1,522.00
|
|
| SC117316 | KIF5B (untagged)-Human kinesin family member 5B (KIF5B) | 10 ug |
$1,324.00
|
|
Citations
A human proteogenomic-cellular framework identifies KIF5A as a modulator of astrocyte process integrity with relevance to ALS
Szebényi, Barrio-Hernandez, Gibbons et al
Commun Biol (2023) 6 (1), 678 DOI: 10.1038/s42003-023-05041-4
Szebényi, Barrio-Hernandez, Gibbons et al
Commun Biol (2023) 6 (1), 678 DOI: 10.1038/s42003-023-05041-4
Cited by 12
AI Summary: OriGene’s KIF5B (RC216778) and KIF5C (RC218796) plasmids were used in this study to validate the specificity of the KIF5A antibody by serving as controls in HEK293 cell transfection experiments.
Snippet: To confirm the KIF5A antibody specificity, HEK293 cells were plated onto T25 flasks and grown in DMEM media supplemented with 10% foetal bovine serum (FBS) until they reached 70-80% confluence and were transfected with either KIF5A (Addgene plasmid pSN837, 184825, a gift from Kyoko Chiba), KIF5B (RC216778, Origene) or KIF5C (RC218796, Origene) plasmids using Lipofectamine 2000 reagent (Thermofisher Scientific)
Showing 1-1 of 1 papers.
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in shipping.
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