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DDX42 (NM_203499) Human Tagged ORF Clone

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SKU
RC216636
DDX42 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 42 (DDX42), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $859.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DDX42
Synonyms DDX42P; RHELP; RNAHP; SF3B8; SF3b125
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC216636 representing NM_203499
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACTGGAATAAAGGTGGTCCTGGCACTAAGCGAGGATTTGGCTTTGGAGGTTTTGCCATCAGTGCTG
GGAAAAAGGAGGAACCCAAACTCCCACAGCAGTCCCACAGTGCCTTTGGGGCAACCAGCTCTTCTTCTGG
ATTTGGAAAGTCAGCTCCACCACAGCTTCCTTCTTTCTACAAAATTGGATCTAAGCGGGCCAACTTTGAT
GAAGAAAATGCCTATTTTGAAGATGAGGAAGAAGATTCTAGCAACGTTGATTTACCTTACATTCCTGCTG
AAAACTCACCAACTCGCCAGCAATTCCATTCCAAGCCAGTAGATTCTGACAGCGATGATGATCCCTTGGA
GGCATTCATGGCTGAAGTGGAGGATCAGGCAGCTAGAGACATGAAGAGGCTTGAAGAAAAGGACAAGGAA
AGAAAAAACGTAAAGGGTATTCGAGATGACATTGAAGAGGAAGATGACCAAGAAGCTTATTTTCGATACA
TGGCAGAAAACCCAACTGCTGGTGTGGTTCAGGAGGAAGAGGAAGACAATCTAGAATATGATAGTGACGG
AAATCCAATTGCACCTACCAAAAAAATCATTGATCCTCTTCCCCCCATTGATCATTCAGAGATTGACTAT
CCACCATTTGAAAAAAACTTTTACAATGAGCATGAAGAGATAACCAACCTCACTCCACAGCAGTTAATAG
ATCTCCGGCATAAGCTCAATCTTCGGGTCTCTGGTGCTGCACCTCCTAGACCAGGAAGTAGCTTTGCTCA
TTTTGGGTTTGACGAACAACTTATGCACCAGATTCGGAAATCTGAATACACACAGCCCACTCCAATACAG
TGCCAGGGTGTGCCTGTGGCATTAAGTGGTAGAGACATGATTGGTATTGCCAAAACAGGTAGTGGGAAAA
CTGCAGCCTTCATTTGGCCCATGTTGATTCATATAATGGACCAGAAGGAGTTGGAACCAGGTGATGGACC
AATTGCAGTGATTGTGTGTCCTACCAGGGAGCTTTGCCAGCAGATCCATGCAGAATGTAAGCGGTTTGGA
AAAGCATATAATCTTCGATCAGTGGCCGTATATGGAGGAGGGAGTATGTGGGAGCAGGCCAAGGCCCTTC
AGGAGGGGGCAGAGATTGTTGTGTGTACCCCAGGTCGACTGATAGATCATGTGAAAAAGAAAGCTACCAA
TCTTCAAAGAGTCTCTTACCTTGTGTTTGATGAAGCAGATCGAATGTTTGACATGGGATTTGAGTACCAA
GTTCGATCCATAGCAAGTCATGTTCGTCCTGACAGGCAGACTCTCTTATTTAGTGCAACTTTTCGGAAGA
AGATTGAAAAGTTGGCCAGAGACATCCTGATCGACCCTATTCGAGTGGTGCAGGGAGATATTGGAGAGGC
AAATGAAGATGTGACACAGATTGTGGAGATTCTCCATTCTGGACCTAGTAAATGGAACTGGCTTACCCGG
CGTCTGGTAGAATTTACCTCTTCAGGGAGTGTCCTCCTCTTTGTTACTAAAAAAGCCAATGCTGAAGAGC
TAGCGAATAACCTTAAACAGGAGGGTCATAATCTTGGGCTGCTCCATGGGGATATGGATCAGAGTGAGAG
AAACAAGGTCATTTCAGACTTTAAGAAAAAGGACATCCCAGTCCTGGTGGCCACAGATGTTGCAGCCCGT
GGTCTGGACATTCCTTCAATTAAGACTGTCATTAACTATGATGTGGCACGAGACATTGATACCCACACGC
ATAGGATTGGCCGCACAGGAAGAGCGGGTGAGAAAGGTGTGGCCTATACCCTACTCACTCCCAAGGACAG
CAATTTTGCTGGTGACCTGGTCCGGAACTTGGAAGGAGCCAATCAACACGTTTCTAAGGAACTCCTAGAT
CTGGCAATGCAGAATGCCTGGTTTCGGAAATCTCGATTCAAAGGAGGGAAAGGAAAAAAGCTGAACATTG
GTGGAGGAGGCCTAGGCTACAGGGAGCGGCCTGGCCTGGGCTCTGAGAACATGGATCGAGGAAATAACAA
TGTAATGAGCAATTATGAGGCCTACAAGCCTTCCACAGGAGCTATGGGAGATCGACTAACGGCAATGAAA
GCAGCTTTCCAGTCACAGTACAAGAGTCACTTTGTTGCAGCCAGTTTAAGTAATCAGAAGGCTGGAAGTT
CTGCTGCTGGGGCAAGTGGGTGGACTAGTGCAGGGAGCTTGAATTCTGTTCCAACTAACTCAGCACAACA
GGGCCATAACAGTCCTGACAGCCCCGTCACCAGTGCCGCCAAGGGCATCCCAGGCTTTGGCAATACTGGC
AACATCAGTGGTGCCCCTGTGACCTACCCGTCTGCCGGAGCCCAAGGAGTCAACAACACAGCTTCAGGGA
ATAACAGCCGAGAAGGGACTGGGGGCAGCAACGGGAAAAGAGAGAGATATACTGAGAACCGGGGCAGCAG
CCGTCACAGTCACGGAGAGACTGGCAATCGGCATAGCGATAGTCCACGTCACGGAGATGGTGGTCGCCAT
GGAGATGGATACCGCCATCCAGAAAGCAGCAGCCGTCATACTGATGGCCATCGGCACGGGGAGAACAGAC
ATGGAGGAAGCGCAGGCCGGCATGGGGAGAACCGGGGTGCAAATGATGGTCGGAATGGGGAAAGCAGGAA
AGAAGCTTTTAATCGTGAGAGCAAGATGGAGCCCAAGATGGAACCCAAAGTGGACAGCAGCAAGATGGAC
AAGGTGGACAGCAAGACAGATAAGACAGCTGACGGCTTTGCTGTCCCAGAGCCGCCTAAACGCAAGAAAA
GTCGATGGGACAGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC216636 representing NM_203499
Red=Cloning site Green=Tags(s)

MNWNKGGPGTKRGFGFGGFAISAGKKEEPKLPQQSHSAFGATSSSSGFGKSAPPQLPSFYKIGSKRANFD
EENAYFEDEEEDSSNVDLPYIPAENSPTRQQFHSKPVDSDSDDDPLEAFMAEVEDQAARDMKRLEEKDKE
RKNVKGIRDDIEEEDDQEAYFRYMAENPTAGVVQEEEEDNLEYDSDGNPIAPTKKIIDPLPPIDHSEIDY
PPFEKNFYNEHEEITNLTPQQLIDLRHKLNLRVSGAAPPRPGSSFAHFGFDEQLMHQIRKSEYTQPTPIQ
CQGVPVALSGRDMIGIAKTGSGKTAAFIWPMLIHIMDQKELEPGDGPIAVIVCPTRELCQQIHAECKRFG
KAYNLRSVAVYGGGSMWEQAKALQEGAEIVVCTPGRLIDHVKKKATNLQRVSYLVFDEADRMFDMGFEYQ
VRSIASHVRPDRQTLLFSATFRKKIEKLARDILIDPIRVVQGDIGEANEDVTQIVEILHSGPSKWNWLTR
RLVEFTSSGSVLLFVTKKANAEELANNLKQEGHNLGLLHGDMDQSERNKVISDFKKKDIPVLVATDVAAR
GLDIPSIKTVINYDVARDIDTHTHRIGRTGRAGEKGVAYTLLTPKDSNFAGDLVRNLEGANQHVSKELLD
LAMQNAWFRKSRFKGGKGKKLNIGGGGLGYRERPGLGSENMDRGNNNVMSNYEAYKPSTGAMGDRLTAMK
AAFQSQYKSHFVAASLSNQKAGSSAAGASGWTSAGSLNSVPTNSAQQGHNSPDSPVTSAAKGIPGFGNTG
NISGAPVTYPSAGAQGVNNTASGNNSREGTGGSNGKRERYTENRGSSRHSHGETGNRHSDSPRHGDGGRH
GDGYRHPESSSRHTDGHRHGENRHGGSAGRHGENRGANDGRNGESRKEAFNRESKMEPKMEPKVDSSKMD
KVDSKTDKTADGFAVPEPPKRKKSRWDS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_203499
ORF Size 2814 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_203499.3
RefSeq Size 3943 bp
RefSeq ORF 2817 bp
Locus ID 11325
UniProt ID Q86XP3
Cytogenetics 17q23.3
Protein Pathways Spliceosome
MW 103 kDa
Summary This gene encodes a member of the Asp-Glu-Ala-Asp (DEAD) box protein family. Members of this protein family are putative RNA helicases, and are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Two transcript variants encoding the same protein have been identified for this gene. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_203499" in other vectors (4)
SKU Description Size Price
RC216636L3 Lenti-ORF clone of DDX42 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 42 (DDX42), transcript variant 2 10 ug
$1,159.00
RC216636L4 Lenti-ORF clone of DDX42 (mGFP-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 42 (DDX42), transcript variant 2 10 ug
$1,159.00
RG216636 DDX42 (tGFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 42 (DDX42), transcript variant 2 10 ug
$1,059.00
SC308214 DDX42 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 42 (DDX42), transcript variant 2 10 ug
$946.00

Plasmid Map for RC216636

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC216636
Circular map for RC216636

file-alt Citations

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