MAPK15 (NM_139021) Human Tagged ORF Clone
$506.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | MAPK15 |
| Synonyms | ERK7; ERK8 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_139021 |
| ORF Size | 1632 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_139021.3 |
| RefSeq Size | 1904 bp |
| RefSeq ORF | 1635 bp |
| Locus ID | 225689 |
| UniProt ID | Q8TD08 |
| Cytogenetics | 8q24.3 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 60.3 kDa |
| Summary | Atypical MAPK protein that regulates several process such as autophagy, ciliogenesis, protein trafficking/secretion and genome integrity, in a kinase activity-dependent manner (PubMed:22948227, PubMed:24618899, PubMed:29021280, PubMed:21847093, PubMed:20733054). Controls both, basal and starvation-induced autophagy throught its interaction with GABARAP, MAP1LC3B and GABARAPL1 leading to autophagosome formation, SQSTM1 degradation and reduced MAP1LC3B inhibitory phosphorylation (PubMed:22948227). Regulates primary cilium formation and the localization of ciliary proteins involved in cilium structure, transport, and signaling (PubMed:29021280). Prevents the relocation of the sugar-adding enzymes from the Golgi to the endoplasmic reticulum, thereby restricting the production of sugar-coated proteins (PubMed:24618899). Upon amino-acid starvation, mediates transitional endoplasmic reticulum site disassembly and inhibition of secretion (PubMed:21847093). Binds to chromatin leading to MAPK15 activation and interaction with PCNA, that which protects genomic integrity by inhibiting MDM2-mediated degradation of PCNA (PubMed:20733054). Regulates DA transporter (DAT) activity and protein expression via activation of RhoA (PubMed:28842414). In response to H(2)O(2) treatment phosphorylates ELAVL1, thus preventing it from binding to the PDCD4 3' UTR and rendering the PDCD4 mRNA accessible to miR-21 and leading to its degradation and loss of protein expression (PubMed:26595526). Also functions in a kinase activity-independent manner as a negative regulator of growth (By similarity). Phosphorylates in vitro FOS and MBP (PubMed:11875070, PubMed:16484222, PubMed:20638370, PubMed:19166846). During oocyte maturation, plays a key role in the microtubule organization and meiotic cell cycle progression in oocytes, fertilized eggs, and early embryos (By similarity). Interacts with ESRRA promoting its re-localization from the nucleus to the cytoplasm and then prevents its transcriptional activity (PubMed:21190936).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_139021" in other vectors (8)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC216589L1 | Lenti ORF clone of Human mitogen-activated protein kinase 15 (MAPK15), Myc-DDK-tagged | 10 ug |
$806.00
|
|
| RC216589L2 | Lenti ORF clone of Human mitogen-activated protein kinase 15 (MAPK15), mGFP tagged | 10 ug |
$806.00
|
|
| RC216589L3 | Lenti ORF clone of Human mitogen-activated protein kinase 15 (MAPK15), Myc-DDK-tagged | 10 ug |
$806.00
|
|
| RC216589L4 | Lenti ORF clone of Human mitogen-activated protein kinase 15 (MAPK15), mGFP tagged | 10 ug |
$806.00
|
|
| RG216589 | MAPK15 (tGFP-tagged) - Human mitogen-activated protein kinase 15 (MAPK15) | 10 ug |
$489.00
$706.00
|
|
| SC120623 | MAPK15 (untagged)-Human mitogen-activated protein kinase 15 (MAPK15) | 10 ug |
$300.00
|
|
| SC317926 | MAPK15 (untagged)-Human mitogen-activated protein kinase 15 (MAPK15) | 10 ug |
$558.00
|
|
| SC323484 | MAPK15 (untagged)-Kinase deficient mutant (K42M) of Human mitogen-activated protein kinase 15 (MAPK15) | 10 ug |
$300.00
|
|
Citations
ERK8 is a negative regulator of O-GalNAc glycosylation and cell migration
Chia, Tham, Gill et al
Elife (2014) 3, e01828
Chia, Tham, Gill et al
Elife (2014) 3, e01828
AI Summary: OriGene contributed to this study by providing the human MAPK15/ERK8 cDNA (product #RG216589), which was used to generate constructs for investigating ERK8’s role in O-GalNAc glycosylation and cell migration.
Snippet: supplemented with 15% FBS. All cells were grown at 37°C in a 10% CO2 incubator. Human MAPK15/ ERK8 (NM_139021) was amplified from cDNA purchased from Origene (#RG216589;Rockville, MD) by PCR and cloned into entry vector pDONR221 (Invitrogen, Life Technologies Corporation, Carlsbad, CA). The catalytically inactive ERK8 mutant construct was generated by
Showing 1-1 of 1 papers.
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