CD35 (CR1) (NM_000573) Human Tagged ORF Clone
- TrueORF®
SKU
RC216533
CR1 (Myc-DDK-tagged)-Human complement component (3b/4b) receptor 1 (Knops blood group) (CR1), transcript variant F
$2,692.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | CD35 |
| Synonyms | C3BR; C4BR; CD35; KN |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_000573 |
| ORF Size | 6117 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000573.4 |
| RefSeq Size | 8629 bp |
| RefSeq ORF | 6120 bp |
| Locus ID | 1378 |
| UniProt ID | P17927 |
| Cytogenetics | 1q32.2 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Complement and coagulation cascades, Hematopoietic cell lineage |
| MW | 223.7 kDa |
| Summary | This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The genome is polymorphic at this locus with allele-specific splice variants encoding different isoforms, based on the presence/absence of long homologous repeats (LHRs). The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in this gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus, sarcoidosis and Alzheimer's disease. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. provided by RefSeq, May 2020 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000573" in other vectors (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC216533L1 | Lenti ORF clone of Human complement component (3b/4b) receptor 1 (Knops blood group) (CR1), transcript variant F, Myc-DDK-tagged | 10 ug |
$2,992.00
|
|
| RG216533 | CR1 (tGFP-tagged) - Human complement component (3b/4b) receptor 1 (Knops blood group) (CR1), transcript variant F | 10 ug |
$2,892.00
|
|
| SC300100 | CR1 (untagged)-Human complement component (3b/4b) receptor 1 (Knops blood group) (CR1), transcript variant F | 10 ug |
$2,693.00
|
|
Citations
Complement Receptor 1 (CR1/CD35)-expressing retinal pigment epithelial cells as a potential therapy for age-related macular degeneration
Simmons, Mazzilli, Mueller-Ortiz et al
Mol. Immunol. (2019) 118, 91-98 DOI: 10.1016/j.molimm.2019.11.007
Simmons, Mazzilli, Mueller-Ortiz et al
Mol. Immunol. (2019) 118, 91-98 DOI: 10.1016/j.molimm.2019.11.007
Cited by 8
AI Summary: OriGene contributed to this study by providing the CR1 expression plasmid (Catalog #RC216533), which was used to generate retinal pigment epithelial cells overexpressing CR1 for functional analysis as a potential therapy for age-related macular degeneration.
Snippet: Briefly, ARPE19 were transfected with a plasmid containing CR1, a CMV promoter, and a G418 resistance gene (Catalog #RC216533, Origene, Rockville, MD) using an Amaxa Nucleofector II (Lonza, Basel, Switzerland) and Cell Line Nucleofector Kit V (Lonza);- Briefly, ARPE19 were transfected with a plasmid containing CR1, a CMV promoter, and a G418 resistance gene (Catalog #RC216533, Origene, Rockville, MD) using an Amaxa Nucleofector II (Lonza, Basel, Switzerland) and Cell Line Nucleofector Kit V (Lonza
Showing 1-1 of 1 papers.
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