DCAF12L2 (NM_001013628) Human Tagged ORF Clone

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SKU
RC216379
DCAF12L2 (Myc-DDK-tagged)-Human DDB1 and CUL4 associated factor 12-like 2 (DCAF12L2)
  $457.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DCAF12L2
Synonyms WDR40C
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC216379 representing NM_001013628
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCAGCAGCAAACAGGTAGCAGGAAACGGAAAGCGCCCGCGGTCGAGGCGGGAGCCGGGAGCTCGT
CGTCGCAGGGTTTAGCGGCGGCGGACGGAGAGGGGCCTCTGCTACCCAAGAAGCAGAAGCGGCCGGCGAC
GCGTCGCAGGCTGGTGCACTATCTGAAGGGCCGGGAGGTAGGAGCGCGGGGCCCAGCCGGGCTCCAGGGC
TTCGAGGGCGAGCTGCGGGGCTACGCCGTCCAGAGGCTGCCCGAGCTGCTGACGGAGCGCCAGCTGGACC
TGGGCACCCTCAACAAGGTGTTCGCGTCACAGTGGCTGAACGCCAGGCAGGTGGTGTGCGGCACCAAGTG
TAATACGCTGTTTGTGGTGGACGTGCAGTCAGGCCACATCACGCGCATCCCCCTCATGCGGGACAAGGAG
GCCGGGCTGGCCCAGGCCCACCAGGGCTGCGGCATCCATGCCATCGAGCTGAATCCCTCCAAGACGCTTC
TGGCCACCGGCGGCGAAAACCCCAACAGCCTGGCCATCTACCAGCTGCCCACCCTGGACCCCCTGTGCCT
GGGCGACCGCCATGGCCACAAGGACTGGATCTTCGCAGTCGCCTGGCTGAGTGACACCGTAGCTGTGAGC
GGCTCCCGCGACGGCACCGTGGCTCTGTGGCGGATGGACCCAGACATGTTTAATGGCAGCATTGCCTGGC
ACAGCGAGGTGGGTCTCCCAGTATATGCCCACATCCGTCCGAGGGATGTGGAGGCCATCCCCAGGGCCAG
CACCAACCCCAGCAACCGCAAGGTACGGGCCCTGGCCTTCAGCGGCAAGAACCAGGAGCTGGGAGCGGTG
TCCTTGGACGGCTACTTCCACCTGTGGAAAGCCCGGAGCACACTATCCAGGCTCCTGTCCATCAGGCTGC
CCTACTGCCGAGAGAATGTGTGCCTGACCTACTGTGATGAGTTGTCCCTGTACGCTGTGGGCTCTCAGTC
CCACGTCTCCTTCCTGGATCCGCGCCAGCGCCAGCAGAACATCCGGCCCCTGTGCTCTCGAGAGGGTGGC
ACAGGCGTGCGGTCGCTGAGCTTCTACCAGCACATCATCACTGTGGGCACCGGCCATGGTTCCCTGCTCT
TCTATGACATCCGCGCCCAGAAGTTCCTGGAGGAGAGGGCCTCTTCCAGCCTGGACTCCATGCCGGGGCC
CGCAGGGAGGAAGCTCAAGCTTGCCTGCGGCAGAGGCTGGCTCAACCAAGATGACGTCTGGGTGAACTAC
TTTGGTGGCATGGGAGAGTTCCCCAATGCGCTCTACACCCACTGCTACAACTGGCCGGAGATGAAGCTCT
TTGTGGCTGGGGGGCCTCTCCCTTCAGGCCTCCATGGGAACTACGCAGGCCTCTGGAGT


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC216379 representing NM_001013628
Red=Cloning site Green=Tags(s)

MAQQQTGSRKRKAPAVEAGAGSSSSQGLAAADGEGPLLPKKQKRPATRRRLVHYLKGREVGARGPAGLQG
FEGELRGYAVQRLPELLTERQLDLGTLNKVFASQWLNARQVVCGTKCNTLFVVDVQSGHITRIPLMRDKE
AGLAQAHQGCGIHAIELNPSKTLLATGGENPNSLAIYQLPTLDPLCLGDRHGHKDWIFAVAWLSDTVAVS
GSRDGTVALWRMDPDMFNGSIAWHSEVGLPVYAHIRPRDVEAIPRASTNPSNRKVRALAFSGKNQELGAV
SLDGYFHLWKARSTLSRLLSIRLPYCRENVCLTYCDELSLYAVGSQSHVSFLDPRQRQQNIRPLCSREGG
TGVRSLSFYQHIITVGTGHGSLLFYDIRAQKFLEERASSSLDSMPGPAGRKLKLACGRGWLNQDDVWVNY
FGGMGEFPNALYTHCYNWPEMKLFVAGGPLPSGLHGNYAGLWS

SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_001013628
ORF Size 1389 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001013628.3
RefSeq Size 1421 bp
RefSeq ORF 1392 bp
Locus ID 340578
UniProt ID Q5VW00
Cytogenetics Xq25
MW 50.6 kDa
Summary This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by Gly-His and Trp-Asp (GH-WD), which may facilitate formation of heterotrimeric or multi-protein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene appears to represent an intronless retrocopy of a related multi-exon gene located on chromosome 9. However, the CDS of this intronless gene remains intact, it is conserved in other mammalian species, it is known to be transcribed, and it is therefore thought to encode a functional protein. provided by RefSeq, May 2010
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Documents
Resources
"NM_001013628" in other vectors (4)
SKU Description Size Price
RC216379L3 Lenti ORF clone of Human DDB1 and CUL4 associated factor 12-like 2 (DCAF12L2), Myc-DDK-tagged 10 ug
$757.00
RC216379L4 Lenti ORF clone of Human DDB1 and CUL4 associated factor 12-like 2 (DCAF12L2), mGFP tagged 10 ug
$757.00
RG216379 DCAF12L2 (tGFP-tagged) - Human DDB1 and CUL4 associated factor 12-like 2 (DCAF12L2) 10 ug
$657.00
SC301803 DCAF12L2 (untagged)-Human DDB1 and CUL4 associated factor 12-like 2 (DCAF12L2) 10 ug
$503.00

Plasmid Map for RC216379

Download sequence for

Cloning scheme for RC216379

Circular map for RC216379

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