DCAF12L2 (NM_001013628) Human Tagged ORF Clone
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | DCAF12L2 |
| Synonyms | WDR40C |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
![]() Sequencher program is needed, download here |
| Restriction Sites |
SgfI-RsrII Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001013628 |
| ORF Size | 1389 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001013628.3 |
| RefSeq Size | 1421 bp |
| RefSeq ORF | 1392 bp |
| Locus ID | 340578 |
| UniProt ID | Q5VW00 |
| Cytogenetics | Xq25 |
| MW | 50.6 kDa |
| Summary | This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by Gly-His and Trp-Asp (GH-WD), which may facilitate formation of heterotrimeric or multi-protein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene appears to represent an intronless retrocopy of a related multi-exon gene located on chromosome 9. However, the CDS of this intronless gene remains intact, it is conserved in other mammalian species, it is known to be transcribed, and it is therefore thought to encode a functional protein. provided by RefSeq, May 2010 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001013628" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC216379L3 | Lenti ORF clone of Human DDB1 and CUL4 associated factor 12-like 2 (DCAF12L2), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC216379L4 | Lenti ORF clone of Human DDB1 and CUL4 associated factor 12-like 2 (DCAF12L2), mGFP tagged | 10 ug |
$757.00
|
|
| RG216379 | DCAF12L2 (tGFP-tagged) - Human DDB1 and CUL4 associated factor 12-like 2 (DCAF12L2) | 10 ug |
$657.00
|
|
| SC301803 | DCAF12L2 (untagged)-Human DDB1 and CUL4 associated factor 12-like 2 (DCAF12L2) | 10 ug |
$503.00
|
|
Citations
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