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FBXO43 (NM_001029860) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC216347L3
Lenti-ORF clone of FBXO43 (Myc-DDK-tagged)-Human F-box protein 43 (FBXO43), transcript variant 2
ORF Plasmid:
DDK DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $948.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FBXO43
Synonyms EMI2; ERP1; FBX43
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC216347).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001029860
ORF Size 2124 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001029860.2
RefSeq Size 3211 bp
RefSeq ORF 2127 bp
Locus ID 286151
UniProt ID Q4G163
Cytogenetics 8q22.2
Protein Pathways Oocyte meiosis
MW 78.2 kDa
Summary Members of the F-box protein family, such as FBXO43, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 PubMed 15520277).supplied by OMIM, Mar 2008
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Documents
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"NM_001029860" in other vectors (4)
SKU Description Size Price
RC216347 FBXO43 (Myc-DDK-tagged)-Human F-box protein 43 (FBXO43), transcript variant 2 10 ug
$648.00
RC216347L4 Lenti-ORF clone of FBXO43 (mGFP-tagged)-Human F-box protein 43 (FBXO43), transcript variant 2 10 ug
$948.00
RG216347 FBXO43 (tGFP-tagged) - Human F-box protein 43 (FBXO43), transcript variant 2 10 ug
$848.00
SC318190 FBXO43 (untagged)-Human F-box protein 43 (FBXO43), transcript variant 2 10 ug
$714.00

Plasmid Map for RC216347L3

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Cloning scheme for RC216347L3
Circular map for RC216347L3

file-alt Citations

FBXO43 promotes cell cycle progression in cancer cells through stabilizing SKP2
Zheng, Shen, Chen et al
Cancer Lett (2024) 591, 216848 DOI: 10.1016/j.canlet.2024.216848
Cited by 17
AI Summary: OriGene’s FBXO43 plasmid (#RC216347L3) was used in this study to investigate the role of FBXO43 in promoting cell cycle progression and growth in cancer cells.
Snippet: XO43 (#RC216347L3) plasmids were acquired from OriGene Technologies. … FBXW2 shRNA (#sc-92515-SH) plasmid was acquired from Santa Cruz Biotechnology. pcDNA3.1-Myc-CDH1, pcDNA4.0-FLAG-FBXO43 deletion mutants and pLKO.1-shFBXO43 plasmids (target sequence: 5′-GCTTAACACATCC-TTTAGAAT-3′) were constructed in our lab. …
Showing 1-1 of 1 papers.
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