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AMELX (NM_001142) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC216306
AMELX (Myc-DDK-tagged)-Human amelogenin, X-linked (AMELX), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "AMELX or NM_001142"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol AMELX
Synonyms AI1E; AIH1; ALGN; AMG; AMGL; AMGX
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC216306 representing NM_001142
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGACCTGGATTTTATTTGCCTGCCTCCTGGGAGCAGCTTTTGCCATGCCTCTACCACCTCATCCTG
GGCACCCTGGTTATATCAACTTCAGCTATGAGGTGCTTACCCCTTTGAAGTGGTACCAGAGCATAAGGCC
ACCGTACCCTTCCTATGGTTACGAGCCCATGGGTGGATGGCTGCACCACCAAATCATCCCCGTGCTGTCC
CAACAGCACCCCCCGACTCACACCCTGCAGCCTCATCACCACATCCCAGTGGTGCCAGCTCAGCAGCCCG
TGATCCCCCAGCAACCAATGATGCCCGTTCCTGGCCAACACTCCATGACTCCAATCCAACACCACCAGCC
AAACCTCCCTCCGCCCGCCCAGCAGCCCTACCAGCCCCAGCCTGTTCAGCCACAGCCTCACCAGCCCATG
CAGCCCCAGCCACCTGTGCACCCCATGCAGCCCCTGCCGCCACAGCCACCTCTGCCTCCGATGTTCCCCA
TGCAGCCCCTGCCTCCCATGCTTCCTGATCTGACTCTGGAAGCTTGGCCATCAACAGACAAGACCAAGCG
GGAGGAAGTGGAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC216306 representing NM_001142
Red=Cloning site Green=Tags(s)

MGTWILFACLLGAAFAMPLPPHPGHPGYINFSYEVLTPLKWYQSIRPPYPSYGYEPMGGWLHHQIIPVLS
QQHPPTHTLQPHHHIPVVPAQQPVIPQQPMMPVPGQHSMTPIQHHQPNLPPPAQQPYQPQPVQPQPHQPM
QPQPPVHPMQPLPPQPPLPPMFPMQPLPPMLPDLTLEAWPSTDKTKREEVD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001142
ORF Size 573 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001142.2, NP_001133.1
RefSeq Size 793 bp
RefSeq ORF 576 bp
Locus ID 265
UniProt ID Q99217
Cytogenetics Xp22.2
Protein Families Druggable Genome, Secreted Protein, Transmembrane
MW 21.6 kDa
Summary This gene encodes a member of the amelogenin family of extracellular matrix proteins. Amelogenins are involved in biomineralization during tooth enamel development. Mutations in this gene cause X-linked amelogenesis imperfecta. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Jul 2008
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"NM_001142" in other vectors (4)
SKU Description Size Price
RC216306L3 Lenti-ORF clone of AMELX (Myc-DDK-tagged)-Human amelogenin, X-linked (AMELX), transcript variant 1 10 ug
$600.00
RC216306L4 Lenti-ORF clone of AMELX (mGFP-tagged)-Human amelogenin, X-linked (AMELX), transcript variant 1 10 ug
$600.00
RG216306 AMELX (tGFP-tagged) - Human amelogenin, X-linked (AMELX), transcript variant 1 10 ug
$500.00
SC302979 AMELX (untagged)-Human amelogenin, X-linked (AMELX), transcript variant 1 10 ug
$330.00

Plasmid Map for RC216306

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for RC216306
Circular map for RC216306

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