ST3GAL4 (NM_006278) Human Tagged Lenti ORF Clone
- LentiORF®
SKU
RC216090L3
Lenti-ORF clone of ST3GAL4 (Myc-DDK-tagged)-Human ST3 beta-galactoside alpha-2,3-sialyltransferase 4 (ST3GAL4)
$750.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | ST3GAL4 |
| Synonyms | CGS23; gal-NAc6S; NANTA3; SAT3; SIAT4; SIAT4C; ST-4; ST3GalA.2; ST3GalIV; STZ |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data | |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene | |
| ACCN | NM_006278 |
| ORF Size | 987 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_006278.1 |
| RefSeq Size | 1766 bp |
| RefSeq ORF | 990 bp |
| Locus ID | 6484 |
| UniProt ID | Q11206 |
| Cytogenetics | 11q24.2 |
| Domains | Glyco_transf_29 |
| Protein Families | Secreted Protein, Transmembrane |
| Protein Pathways | Glycosphingolipid biosynthesis - lacto and neolacto series, Metabolic pathways |
| MW | 37.3 kDa |
| Summary | This gene encodes a member of the glycosyltransferase 29 family, a group of enzymes involved in protein glycosylation. The encoded protein is targeted to Golgi membranes but may be proteolytically processed and secreted. The gene product may also be involved in the increased expression of sialyl Lewis X antigen seen in inflammatory responses. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Dec 2011 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_006278" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC216090 | ST3GAL4 (Myc-DDK-tagged)-Human ST3 beta-galactoside alpha-2,3-sialyltransferase 4 (ST3GAL4) | 10 ug |
$450.00
|
|
| RC216090L1 | Lenti-ORF clone of ST3GAL4 (Myc-DDK-tagged)-Human ST3 beta-galactoside alpha-2,3-sialyltransferase 4 (ST3GAL4) | 10 ug |
$750.00
|
|
| RC216090L2 | Lenti-ORF clone of ST3GAL4 (mGFP-tagged)-Human ST3 beta-galactoside alpha-2,3-sialyltransferase 4 (ST3GAL4) | 10 ug |
$750.00
|
|
| RC216090L4 | Lenti-ORF clone of ST3GAL4 (mGFP-tagged)-Human ST3 beta-galactoside alpha-2,3-sialyltransferase 4 (ST3GAL4) | 10 ug |
$750.00
|
|
| RG216090 | ST3GAL4 (tGFP-tagged) - Human ST3 beta-galactoside alpha-2,3-sialyltransferase 4 (ST3GAL4) | 10 ug |
$650.00
|
|
| SC312986 | ST3GAL4 (untagged)-Human ST3 beta-galactoside alpha-2,3-sialyltransferase 4 (ST3GAL4) | 10 ug |
$300.00
|
|
Citations
ST6Gal-I-mediated sialylation of the epidermal growth factor receptor modulates cell mechanics and enhances invasion
Rao, Beggs, Ankenbauer et al
J Biol Chem (2022) 298 (4), 101726 DOI: 10.1016/j.jbc.2022.101726
Rao, Beggs, Ankenbauer et al
J Biol Chem (2022) 298 (4), 101726 DOI: 10.1016/j.jbc.2022.101726
Cited by 43
AI Summary: OriGene’s ST3Gal-4 OE lentiviral vector (cat # RC216090L3V) was used to transduce Cos-7 cells, enabling the study of how specific sialyltransferase activity affects cell mechanics and EGFR-mediated invasion.
Snippet: omycin-resistance gene expression. Transduced cells were selected with 10 μg/ml of puromycin (Sigma). For ST3Gal-4 transduction, WT Cos-7 cells were transduced with ST3Gal-4 OE lentiviral vector (OriGene, cat # RC216090L3V) for 16 h at MOI 5 with 8 μg/ml of polybrene (Vector Builder) in serum-free media. Virus-containing media was replaced with fresh complete media and incubated for 48 h
Showing 1-1 of 1 papers.
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