BLM (NM_000057) Human Tagged Lenti ORF Clone
- LentiORF®
SKU
RC216075L4
Lenti ORF clone of Human Bloom syndrome, RecQ helicase-like (BLM), mGFP tagged
$2,118.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | BLM |
| Synonyms | BS; MGRISCE1; RECQ2; RECQL2; RECQL3 |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data | |
| Restriction Sites |
AscI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_000057 |
| ORF Size | 4251 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000057.1 |
| RefSeq Size | 4437 bp |
| RefSeq ORF | 4254 bp |
| Locus ID | 641 |
| UniProt ID | P54132 |
| Cytogenetics | 15q26.1 |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Protein Pathways | Homologous recombination |
| MW | 158.8 kDa |
| Summary | The Bloom syndrome is an autosomal recessive disorder characterized by growth deficiency, microcephaly and immunodeficiency among others. It is caused by homozygous or compound heterozygous mutation in the gene encoding DNA helicase RecQ protein on chromosome 15q26. This Bloom-associated helicase unwinds a variety of DNA substrates including Holliday junction, and is involved in several pathways contributing to the maintenance of genome stability. Identification of pathogenic Bloom variants is required for heterozygote testing in at-risk families. provided by RefSeq, May 2020 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000057" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC216075 | BLM (Myc-DDK-tagged)-Human Bloom syndrome, RecQ helicase-like (BLM) | 10 ug |
$1,818.00
|
|
| RC216075L1 | Lenti ORF clone of Human Bloom syndrome, RecQ helicase-like (BLM), Myc-DDK-tagged | 10 ug |
$2,118.00
|
|
| RC216075L2 | Lenti ORF clone of Human Bloom syndrome, RecQ helicase-like (BLM), mGFP tagged | 10 ug |
$2,118.00
|
|
| RC216075L3 | Lenti ORF clone of Human Bloom syndrome, RecQ helicase-like (BLM), Myc-DDK-tagged | 10 ug |
$2,118.00
|
|
| RG216075 | BLM (tGFP-tagged) - Human Bloom syndrome, RecQ helicase-like (BLM) | 10 ug |
$2,018.00
|
|
| SC300002 | BLM (untagged)-Human Bloom syndrome, RecQ helicase-like (BLM) | 10 ug |
$1,819.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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