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SPHKAP (NM_030623) Human Tagged ORF Clone

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SKU
RC215958
SPHKAP (Myc-DDK-tagged)-Human SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 2
ORF Plasmid:
DDK tGFP
  $1,404.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SPHKAP
Synonyms SKIP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC215958 representing NM_030623
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGGCAACTCCCTGCTCTCGGTACCAAGCAACTTGGAGTCATCACGGATGTATGACGTTTTGGAAC
CGCAGCAGGGCAGAGGCTGTGGCAGCTCAGGAAGCGGCCCGGGGAACTCCATCACAGCCTGTAAGAAGGT
TCTTCGCAGCAATAGCCTGCTGGAGTCAACAGACTACTGGTTGCAGAATCAGAGGATGCCCTGCCAAATT
GGTTTTGTAGAAGACAAGTCTGAAAACTGTGCTTCTGTCTGCTTTGTGAATCTTGATGTGAACAAGGATG
AATGCAGCACAGAGCACCTGCAACAGAAACTGGTCAACGTTTCACCAGATCTTCCAAAACTTATCAGTTC
CATGAATGTCCAACAACCAAAAGAAAATGAAATTGTTGTCCTAAGTGGGTTAGCCTCTGGAAATCTCCAG
GCAGATTTTGAAGTTTCACAGTGCCCTTGGCTGCCAGATATCTGCTTGGTCCAATGTGCAAGAGGGAACA
GACCAAACAGTACCAACTGCATCATCTTTGAAATCAACAAATTTCTGATTGGTCTGGAACTGGTGCAGGA
GCGACAGCTCCACCTGGAAACAAACATCTTGAAACTGGAGGATGACACGAACTGTTCCTTATCTTCAATC
GAGGAAGACTTTCTCACCGCTTCTGAGCACTTGGAGGAGGAAAGCGAGGTGGATGAATCTAGGAACGATT
ATGAAAATATAAATGTCTCAGCCAATGTTTTGGAAAGTAAACAGCTAAAGGGAGCCACCCAGGTGGAATG
GAATTGCAACAAGGAAAAGTGGCTTTATGCTTTGGAAGACAAATACATCAACAAATATCCCACACCATTG
ATTAAAACAGAACGATCTCCAGAAAACCTAACAAAGAACACAGCCTTGCAGAGTCTAGATCCCTCAGCCA
AGCCATCACAGTGGAAAAGAGAAGCTGTGGGGAATGGGAGACAAGCCACACATTATTATCATTCAGAAGC
TTTTAAAGGTCAAATGGAAAAATCACAGGCACTGTATATTCCAAAAGATGCTTATTTCTCCATGATGGAT
AAAGATGTACCTTCTGCATGTGCTGTGGCAGAGCAGAGAAGCAACCTAAACCCAGGAGACCATGAAGACA
CAAGAAACGCTCTCCCTCCTAGACAAGATGGAGAAGTCACCACTGGCAAGTATGCTACAAATTTAGCAGA
ATCCGTGCTGCAGGATGCATTTATTAGATTATCTCAATCTCAGTCCACATTACCCCAGGAATCTGCAGTC
AGTGTTTCTGTAGGAAGTTCTCTGCTTCCCAGTTGCTATTCCACAAAAGATACAGTGGTTTCTCGGTCAT
GGAATGAGCTCCCCAAAATCGTCGTTGTTCAGAGTCCAGATGGCAGTGATGCTGCCCCACAGCCAGGCAT
CTCCTCCTGGCCTGAGATGGAAGTCTCTGTTGAAACCTCAAGCATCCTCTCTGGAGAGAACTCCAGCAGA
CAACCCCAGAGTGCTCTAGAAGTGGCGTTAGCTTGTGCAGCCACTGTGATTGGAACTATTTCCAGTCCAC
AGGCCACAGAAAGACTCAAAATGGAGCAAGTGGTCTCGAACTTTCCCCCAGGGAGCAGTGGTGCACTGCA
AACTCAAGCACCCCAAGGACTCAAGGAACCTTCCATCAATGAGTACTCCTTTCCATCTGCTTTGTGTGGC
ATGACTCAGGTGGCCAGTGCCGTGGCTGTCTGTGGTCTGGGTGAAAGAGAAGAGGTGACATGCTCAGTGG
CTCCAAGTGGTAGCCTCCCGCCTGCAGCTGAGGCTTCTGAAGCCATGCCCCCACTTTGTGGTTTAGCAAG
CATGGAGCTTGGCAAGGAAGCCATTGCCAAGGGATTGCTCAAGGAGGCTGCTCTGGTTTTAACAAGGCCT
AATACCTACAGCAGCATTGGAGACTTTCTGGACTCCATGAACAGGAGAATCATGGAAACTGCTTCAAAGT
CTCAGACCCTGTGCTCAGAAAATGTCGTCAGGAATGAACTGGCACATACCCTGTCCAATGTTATCCTGAG
GCATTCCATTGATGAAGTTCACCACAAAAATATGATAATCGACCCCAATGACAACAGGCATTCATCTGAA
ATTCTGGACACCTTAATGGAAAGTACAAATCAACTGCTTTTAGATGTGATATGCTTCACGTTCAAGAAGA
TGAGTCATATTGTACGGCTTGGTGAATGTCCTGCTGTCCTTTCTAAGGAGACCATCAGAAGGAGGGAGAC
AGAACCAAGCTGCCAGCCATCTGATCCGGGTGCTAGTCAAGCTTGGACAAAAGCCACTGAATCCTCCAGC
AGCTCTCCACTTAGCAATTCACACAACACGAGTCTTGTCATCAACAATCTTGTGGATGGCATGTATTCAA
AACAAGACAAGGGTGGAGTGAGGCCAGGCCTCTTCAAGAACCCCACGCTGCAGTCACAATTATCACGTAG
TCACAGAGTGCCCGATTCTTCAACTGCTACAACATCCTCCAAGGAAATATATCTGAAAGGAATAGCAGGA
GAGGATACAAAAAGCCCTCATCACAGTGAGAATGAATGCAGAGCCTCTTCCGAAGGACAAAGGTCCCCAA
CGGTCAGCCAGTCCAGAAGTGGTTCCCAGGAGGCTGAGGAGAGTATCCACCCAAACACCCAAGAAAAGTA
CAACTGTGCCACATCTCGCATCAACGAAGTTCAAGTCAACCTGTCCTTGTTAGGGGATGACCTGCTGCTT
CCTGCTCAATCCACGCTTCAAACAAAGCATCCAGACATCTACTGCATTACAGACTTTGCGGAAGAATTAG
CAGACACGGTCGTCTCCATGGCAACTGAAATTGCAGCGATTTGCCTTGACAACTCCAGTGGAAAACAACC
CTGGTTTTGTGCATGGAAAAGAGGGAGTGAGTTTCTGATGACACCCAACGTACCCTGCCGATCCTTGAAG
AGGAAGAAAGAGAGCCAGGGGAGCGGGACCGCTGTGAGGAAACACAAGCCTCCCCGGCTCAGTGAGATCA
AGAGGAAGACGGACGAGCACCCTGAGCTTAAAGAAAAGCTGATGAACAGGGTTGTGGATGAGTCCATGAA
CCTTGAAGATGTCCCAGATTCTGTCAATCTTTTTGCCAATGAAGTGGCAGCCAAGATCATGAACCTAACG
GAGTTCTCTATGGTGGACGGCATGTGGCAGGCGCAGGGCTATCCCCGGAATCGGTTACTGAGTGGCGACA
GGTGGAGCCGGCTGAAGGCCTCCAGCTGCGAAAGCATTCCTGAGGAAGACTCCGAGGCCAGGGCCTATGT
CAACAGCCTGGGCTTAATGAGCACGCTGAGCCAGCCGGTCAGCAGGGCCAGCTCTGTCTCCAAGCAGTCG
AGCTGTGAGAGCATCACCGATGAGTTTTCCAGGTTCATGGTGAACCAGATGGAAAATGAAGGGAGAGGAT
TTGAGTTACTGCTGGATTACTATGCTGGCAAGAACGCCAGCAGCATTCTGAACTCAGCCATGCAACAGGC
GTGCCGGAAAAGTGACCACCTCAGTGTGAGGCCTAGCTGTCCCTCTAAGCAGTCCAGCACAGAGAGCATC
ACTGAGGAGTTCTACAGGTACATGCTGAGGGACATCGAAAGAGACAGCAGAGAAAGTGCCTCCTCCAGAC
GGAGCAGCCAGGATTGGACAGCCGGCCTGCTGTCTCCTTCTCTGCGATCCCCAGTGTGCCACAGACAGTC
GTCCATGCCAGACAGCAGATCCCCATGCTCCAGGCTGACAGTGAATGTGCCCATCAAAGCCAACTCTTTA
GATGGCTTTGCTCAGAACTGCCCACAAGATTTCCTAAGCGTGCAGCCGGTCAGTAGCGCGTCCTCATCCG
GTCTCTGCAAATCTGACTCTTGCTTGTATCGGAGAGGTGGGACTGACCACATCACCAACATGTTAATTCA
TGAAACGTGGGCTAGCTCCATTGAGGCTCTCATGCGCAAGAACAAAATCATTGTGGATGATGCAGAGGAA
GCTGACACTGAGCCTGTTTCTGGTGGCTCTCCCTCGCAAGCAGAGAAGTGTGCAAATAGATTAGCTGCGA
GCAGGATGTGCAGTGGGCCAACTCTGCTTGTTCAGGAGTCTCTCGATTGCCCGAGGAAAGACTCTGTTAC
CGAATGTAAACAGCCCCCAGTGTCATCTTTGAGCAAAACTGCTTCTCTTACAAACCACAGCCCTTTAGAT
TCTAAAAAAGAAACTTCCTCGTGCCAGGACCCTGTACCAATAAACCACAAAAGGCGATCACTTTGCTCGA
GGGAAGTGCCTTTGATTCAGATTGAAACAGATCAGAGAGAAGCCTGTGCTGGGGAACCTGAACCCTTCCT
TTCCAAAAGCAGCCTCCTAGAGGAAGCAGAAGGGCATTCGAATGACAAAAACATCCCAGATGTGGTGAGA
GGTGGAGACACAGCCGTGAGCGCTTGTCAAATCCATAGTGACAGCCTTGATACCAGAGATGTACCAGAGG
CTGAAGCCTCCACAGAAGCCAGAGCCCCCGATGAGGCCCCCAACCCTCCAAGCAGCAGCGAGGAGAGCAC
AGGCAGCTGGACCCAGCTTGCCAATGAGGAAGACAACCCAGATGACACAAGTAGCTTTCTCCAGCTCAGT
GAGCGATCCATGAGTGAATTAGTAGAAGAAAAGAAGATTCTTAAAGGACAGTCAGAAAGCACAGAGGCAC
CTGCATCTGGACCGCCTACGGGAACAGCCAGCCCCCAGAGGAGCCTGCTGGTGATCAACTTTGACCTGGA
GCCAGAGTGTCCAGATGCCGAGCTCCGAGCCACTCTGCAGTGGATAGCTGCCTCTGAACTGGGGATTCCC
ACCATCTACTTTAAGAAATCTCAGGAAAACAGAATTGAAAAGTTTCTAGATGTCGTGCAGCTGGTTCATC
GGAAGTCCTGGAAAGTGGGTGATATCTTCCATGCAGTTGTCCAGTACTGCAAAATGCATGAGGAGCAGAA
GGATGGGAGACTGAGTCTCTTTGACTGGCTCTTGGAACTGGGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC215958 protein sequence
Red=Cloning site Green=Tags(s)

MDGNSLLSVPSNLESSRMYDVLEPQQGRGCGSSGSGPGNSITACKKVLRSNSLLESTDYWLQNQRMPCQI
GFVEDKSENCASVCFVNLDVNKDECSTEHLQQKLVNVSPDLPKLISSMNVQQPKENEIVVLSGLASGNLQ
ADFEVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLELVQERQLHLETNILKLEDDTNCSLSSI
EEDFLTASEHLEEESEVDESRNDYENINVSANVLESKQLKGATQVEWNCNKEKWLYALEDKYINKYPTPL
IKTERSPENLTKNTALQSLDPSAKPSQWKREAVGNGRQATHYYHSEAFKGQMEKSQALYIPKDAYFSMMD
KDVPSACAVAEQRSNLNPGDHEDTRNALPPRQDGEVTTGKYATNLAESVLQDAFIRLSQSQSTLPQESAV
SVSVGSSLLPSCYSTKDTVVSRSWNELPKIVVVQSPDGSDAAPQPGISSWPEMEVSVETSSILSGENSSR
QPQSALEVALACAATVIGTISSPQATERLKMEQVVSNFPPGSSGALQTQAPQGLKEPSINEYSFPSALCG
MTQVASAVAVCGLGEREEVTCSVAPSGSLPPAAEASEAMPPLCGLASMELGKEAIAKGLLKEAALVLTRP
NTYSSIGDFLDSMNRRIMETASKSQTLCSENVVRNELAHTLSNVILRHSIDEVHHKNMIIDPNDNRHSSE
ILDTLMESTNQLLLDVICFTFKKMSHIVRLGECPAVLSKETIRRRETEPSCQPSDPGASQAWTKATESSS
SSPLSNSHNTSLVINNLVDGMYSKQDKGGVRPGLFKNPTLQSQLSRSHRVPDSSTATTSSKEIYLKGIAG
EDTKSPHHSENECRASSEGQRSPTVSQSRSGSQEAEESIHPNTQEKYNCATSRINEVQVNLSLLGDDLLL
PAQSTLQTKHPDIYCITDFAEELADTVVSMATEIAAICLDNSSGKQPWFCAWKRGSEFLMTPNVPCRSLK
RKKESQGSGTAVRKHKPPRLSEIKRKTDEHPELKEKLMNRVVDESMNLEDVPDSVNLFANEVAAKIMNLT
EFSMVDGMWQAQGYPRNRLLSGDRWSRLKASSCESIPEEDSEARAYVNSLGLMSTLSQPVSRASSVSKQS
SCESITDEFSRFMVNQMENEGRGFELLLDYYAGKNASSILNSAMQQACRKSDHLSVRPSCPSKQSSTESI
TEEFYRYMLRDIERDSRESASSRRSSQDWTAGLLSPSLRSPVCHRQSSMPDSRSPCSRLTVNVPIKANSL
DGFAQNCPQDFLSVQPVSSASSSGLCKSDSCLYRRGGTDHITNMLIHETWASSIEALMRKNKIIVDDAEE
ADTEPVSGGSPSQAEKCANRLAASRMCSGPTLLVQESLDCPRKDSVTECKQPPVSSLSKTASLTNHSPLD
SKKETSSCQDPVPINHKRRSLCSREVPLIQIETDQREACAGEPEPFLSKSSLLEEAEGHSNDKNIPDVVR
GGDTAVSACQIHSDSLDTRDVPEAEASTEARAPDEAPNPPSSSEESTGSWTQLANEEDNPDDTSSFLQLS
ERSMSELVEEKKILKGQSESTEAPASGPPTGTASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIP
TIYFKKSQENRIEKFLDVVQLVHRKSWKVGDIFHAVVQYCKMHEEQKDGRLSLFDWLLELG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_030623
ORF Size 5013 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030623.4
RefSeq Size 6825 bp
RefSeq ORF 5016 bp
Locus ID 80309
UniProt ID Q2M3C7
Cytogenetics 2q36.3
Protein Families Druggable Genome
MW 183.2 kDa
Summary Anchoring protein that binds preferentially to the type I regulatory subunit of c-AMP-dependent protein kinase (PKA type I) and targets it to distinct subcellular compartments. May act as a converging factor linking cAMP and sphingosine signaling pathways. Plays a regulatory role in the modulation of SPHK1.UniProtKB/Swiss-Prot Function
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"NM_030623" in other vectors (2)
SKU Description Size Price
RG215958 SPHKAP (tGFP-tagged) - Human SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 2 10 ug
$1,604.00
SC305270 SPHKAP (untagged)-Human SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 2 10 ug
$1,545.00

file-alt Citations

Repression of sphingosine kinase (SK)-interacting protein (SKIP) in acute myeloid leukemia diminishes SK activity and its re-expression restores SK function
Ghazaly, Miraki-Moud, Smith et al
J. Biol. Chem. (2020) 295 (16), 5496-5508 DOI: 10.1074/jbc.RA119.010467
AI Summary: OriGene contributed to this study by providing the SKIP cDNA clone, which was used to re-express SKIP in leukemia cell lines and investigate its effects on sphingosine kinase activity and apoptosis.
Snippet: SKIP cDNA clone was purchased from Origene and re-cloned into the pIRES Hyg3 expression vector using standard methods. A FLAG tag was added by PCR protocols using High-Fidelity Taq polymerase and confirmed by sequencing
Showing 1-1 of 1 papers.
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