CARD9 (NM_052813) Human Tagged ORF Clone

SKU RC215899

CARD9 (Myc-DDK-tagged)-Human caspase recruitment domain family, member 9 (CARD9), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: CARD9
• Synonyms: CANDF2; hCARD9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215899 representing NM_052813; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTCGGACTACGAGAACGATGACGAGTGCTGGAACGTCCTGGAGGGCTTCCGGGTGACGCTCACCTCGG; TCATCGACCCCTCACGCATCACACCTTACCTGCGGCAGTGCAAGGTCCTGAACCCTGATGATGAGGAGCA; GGTGCTCAGCGACCCCAACCTGGTCATCCGCAAACGGAAAGTGGGTGTGCTCCTGGACATCCTGCAGCGG; ACCGGCCACAAGGGCTACGTGGCCTTCCTCGAGAGCCTGGAGCTCTACTACCCGCAGCTGTACAAGAAGG; TCACAGGCAAGGAGCCGGCCCGCGTCTTCTCCATGATCATCGACGCGTCCGGGGAGTCAGGCCTGACTCA; GCTGCTGATGACTGAGGTCATGAAGCTGCAGAAGAAGGTGCAGGACCTGACCGCGCTGCTGAGCTCCAAA; GATGACTTCATCAAGGAGCTGCGGGTGAAGGACAGCCTGCTGCGCAAGCACCAGGAGCGTGTGCAGAGGC; TCAAGGAGGAGTGCGAGGCCGGCAGCCGCGAGCTCAAGCGCTGCAAGGAGGAGAACTACGACCTGGCCAT; GCGCCTGGCGCACCAGAGTGAGGAGAAGGGCGCCGCGCTCATGCGGAACCGTGACCTGCAGCTGGAGATT; GACCAGCTCAAGCACAGCCTCATGAAGGCCGAGGACGACTGCAAGGTGGAGCGCAAGCACACGCTGAAGC; TCAGGCACGCCATGGAGCAGCGGCCCAGCCAGGAGCTGCTGTGGGAGCTGCAGCAGGAGAAGGCCCTGCT; CCAGGCCCGGGTGCAGGAGCTGGAGGCCTCCGTCCAGGAGGGGAAGCTGGACAGGAGCAGCCCCTACATC; CAGGTACTGGAGGAGGACTGGCGGCAGGCGCTGCGGGACCACCAGGAGCAGGCCAACACCATCTTCTCCC; TGCGCAAGGACCTCCGCCAGGGCGAGGCCCGACGCCTCCGGTGCATGGAGGAGAAGGAGATGTTCGAGCT; GCAGTGCCTGGCACTACGTAAGGACTCCAAGATGTACAAGGACCGCATCGAGGCCATCCTGCTGCAGATG; GAGGAGGTCGCCATTGAGCGGGACCAGGCCATAGCCACGCGGGAGGAGCTGCACGCACAGCACGCCCGGG; GCCTGCAGGAGAAGGACGCGCTGCGCAAGCAGGTGCGGGAGCTGGGCGAGAAGGCGGATGAGCTGCAGCT; GCAGGTGTTCCAGTGTGAGGCGCAGCTACTGGCCGTGGAGGGCAGGCTCAGGCGGCAGCAGCTGGAGACG; CTCGTCCTGAGCTCCGACCTGGAAGATGGCTCACCCAGGAGGTCCCAGGAGCTCTCACTCCCCCAGGACC; TGGAGGACACCCAGCTCTCAGACAAAGGCTGCCTTGCCGGCGGGGGGAGCCCGAAACAGCCCTTTGCAGC; TCTGCACCAGGAGCAGGTTTTGCGGAACCCCCATGACGCAGGCCTGAGCAGCGGGGAGCCGCCCGAGAAG; GAGCGGCGGCGCCTCAAAGAGAGTTTTGAGAACTACCGCAGGAAGCGCGCCCTCAGGAAGATGCAGAAAG; GATGGCGGCAGGGGGAGGAGGACCGGGAGAACACCACGGGCAGCGACAACACCGACACTGAGGGCTCC; ; ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA; TTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215899 representing NM_052813; Red=Cloning site Green=Tags(s); ; MSDYENDDECWNVLEGFRVTLTSVIDPSRITPYLRQCKVLNPDDEEQVLSDPNLVIRKRKVGVLLDILQR; TGHKGYVAFLESLELYYPQLYKKVTGKEPARVFSMIIDASGESGLTQLLMTEVMKLQKKVQDLTALLSSK; DDFIKELRVKDSLLRKHQERVQRLKEECEAGSRELKRCKEENYDLAMRLAHQSEEKGAALMRNRDLQLEI; DQLKHSLMKAEDDCKVERKHTLKLRHAMEQRPSQELLWELQQEKALLQARVQELEASVQEGKLDRSSPYI; QVLEEDWRQALRDHQEQANTIFSLRKDLRQGEARRLRCMEEKEMFELQCLALRKDSKMYKDRIEAILLQM; EEVAIERDQAIATREELHAQHARGLQEKDALRKQVRELGEKADELQLQVFQCEAQLLAVEGRLRRQQLET; LVLSSDLEDGSPRRSQELSLPQDLEDTQLSDKGCLAGGGSPKQPFAALHQEQVLRNPHDAGLSSGEPPEK; ERRRLKESFENYRRKRALRKMQKGWRQGEEDRENTTGSDNTDTEGS; ; TRRLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052813
• ORF Size: 1608 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_052813.5
• RefSeq Size: 2136 bp
• RefSeq ORF: 1611 bp
• Locus ID: 64170
• UniProt ID: Q9H257
• Cytogenetics: 9q34.3
• Protein Families: Druggable Genome
• Protein Pathways: NOD-like receptor signaling pathway
• MW: 62.1 kDa
• Summary: The protein encoded by this gene is a member of the CARD protein family, which is defined by the presence of a characteristic caspase-associated recruitment domain (CARD). CARD is a protein interaction domain known to participate in activation or suppression of CARD containing members of the caspase family, and thus plays an important regulatory role in cell apoptosis. This protein was identified by its selective association with the CARD domain of BCL10, a postive regulator of apoptosis and NF-kappaB activation, and is thought to function as a molecular scaffold for the assembly of a BCL10 signaling complex that activates NF-kappaB. Several alternatively spliced transcript variants have been observed, but their full-length nature is not clearly defined. [provided by RefSeq, Jul 2008]