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FA20A (FAM20A) (NM_017565) Human Tagged ORF Clone

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SKU
RC215864
FAM20A (Myc-DDK-tagged)-Human family with sequence similarity 20, member A (FAM20A), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $504.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FA20A
Synonyms AI1G; AIGFS; FP2747
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC215864 representing NM_017565
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGGGCTGCGCCGGGACCGCCTACTGACTCTGCTGCTGCTGGGCGCGCTGCTCTCCGCCGACCTCT
ACTTCCACCTCTGGCCCCAAGTACAGCGCCAGCTGCGGCCTCGGGAGCGCCCGCGGGGGTGCCCGTGCAC
CGGCCGCGCCTCCTCCCTGGCGCGGGACTCGGCCGCAGCTGCCTCGGACCCCGGCACGATCGTGCACAAC
TTTTCCCGAACCGAGCCCCGGACTGAACCGGCTGGCGGCAGCCACAGCGGGTCGAGCTCCAAGTTGCAGG
CCCTCTTCGCCCACCCGCTGTACAACGTCCCGGAGGAGCCGCCTCTCCTGGGAGCCGAGGACTCACTCCT
GGCCAGCCAGGAGGCGCTGCGGTATTACCGGAGGAAGGTGGCCCGCTGGAACAGGCGACACAAGATGTAC
AGAGAGCAGATGAACCTTACCTCCCTGGACCCCCCACTGCAGCTCCGACTCGAGGCCAGCTGGGTCCAGT
TCCACCTGGGTATTAACCGCCATGGGCTCTACTCCCGGTCCAGCCCTGTTGTCAGCAAACTTCTGCAAGA
CATGAGGCACTTTCCCACCATCAGTGCTGATTACAGTCAAGATGAGAAAGCCTTGCTGGGGGCATGTGAC
TGCACCCAGATTGTGAAACCCAGTGGGGTCCACCTCAAGCTGGTGCTGAGGTTCTCGGATTTCGGGAAGG
CCATGTTCAAACCCATGAGACAGCAGCGAGATGAAGAGACACCAGTGGACTTCTTCTACTTCATTGACTT
TCAGAGACACAATGCTGAGATCGCAGCTTTCCATCTGGACAGGATTCTGGACTTCCGACGGGTGCCGCCA
ACAGTGGGGAGGATAGTAAATGTCACCAAGGAAATCCTAGAGGTCACCAAGAATGAAATCCTGCAGAGTG
TTTTCTTTGTCTCTCCAGCGAGCAACGTGTGCTTCTTCGCCAAGTGTCCATACATGTGCAAGACGGAGTA
TGCTGTCTGTGGCAAACCACACCTGCTGGAGGGTTCCCTCTCTGCCTTCCTGCCGTCCCTCAACCTGGCC
CCCAGGCTGTCTGTGCCCAACCCCTGGATCCGCTCCTACACACTGGCAGGAAAAGAGGAGTGGGAGGTCA
ATCCCCTTTACTGTGACACAGTGAAACAGATCTACCCGTACAACAACAGCCAGCGGCTCCTCAATGTCAT
CGACATGGCCATCTTCGACTTCTTGATAGGGAATATGGACCGGCACCATTATGAGATGTTCACCAAGTTC
GGGGATGATGGGTTCCTTATTCACCTTGACAACGCCAGAGGGTTCGGACGACACTCCCATGATGAAATCT
CCATCCTCTCGCCTCTCTCCCAGTGCTGCATGATAAAAAAGAAAACACTTTTGCACCTGCAGCTGCTGGC
CCAAGCTGACTACAGACTCAGCGATGTGATGCGAGAATCACTGCTGGAAGACCAGCTCAGCCCTGTCCTC
ACTGAACCCCACCTCCTTGCCCTGGATCGAAGGCTCCAAACCATCCTAAGGACAGTGGAGGGGTGCATAG
TGGCCCATGGACAGCAGAGTGTCATAGTCGACGGCCCAGTGGAACAGTCGGCCCCAGACTCTGGCCAGGC
TAACTTGACAAGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC215864 representing NM_017565
Red=Cloning site Green=Tags(s)

MPGLRRDRLLTLLLLGALLSADLYFHLWPQVQRQLRPRERPRGCPCTGRASSLARDSAAAASDPGTIVHN
FSRTEPRTEPAGGSHSGSSSKLQALFAHPLYNVPEEPPLLGAEDSLLASQEALRYYRRKVARWNRRHKMY
REQMNLTSLDPPLQLRLEASWVQFHLGINRHGLYSRSSPVVSKLLQDMRHFPTISADYSQDEKALLGACD
CTQIVKPSGVHLKLVLRFSDFGKAMFKPMRQQRDEETPVDFFYFIDFQRHNAEIAAFHLDRILDFRRVPP
TVGRIVNVTKEILEVTKNEILQSVFFVSPASNVCFFAKCPYMCKTEYAVCGKPHLLEGSLSAFLPSLNLA
PRLSVPNPWIRSYTLAGKEEWEVNPLYCDTVKQIYPYNNSQRLLNVIDMAIFDFLIGNMDRHHYEMFTKF
GDDGFLIHLDNARGFGRHSHDEISILSPLSQCCMIKKKTLLHLQLLAQADYRLSDVMRESLLEDQLSPVL
TEPHLLALDRRLQTILRTVEGCIVAHGQQSVIVDGPVEQSAPDSGQANLTS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017565
ORF Size 1623 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017565.4
RefSeq Size 2267 bp
RefSeq ORF 1626 bp
Locus ID 54757
UniProt ID Q96MK3
Cytogenetics 17q24.2
Protein Families Secreted Protein, Transmembrane
MW 61.2 kDa
Summary This locus encodes a protein that is likely secreted and may function in hematopoiesis. A mutation at this locus has been associated with amelogenesis imperfecta and gingival hyperplasia syndrome. Alternatively spliced transcript variants have been identified. provided by RefSeq, Aug 2011
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Documents
Resources
"NM_017565" in other vectors (6)
SKU Description Size Price
RC215864L1 Lenti ORF clone of Human family with sequence similarity 20, member A (FAM20A), transcript variant 1, Myc-DDK-tagged 10 ug
$804.00
RC215864L2 Lenti ORF clone of Human family with sequence similarity 20, member A (FAM20A), transcript variant 1, mGFP tagged 10 ug
$804.00
RC215864L3 Lenti ORF clone of Human family with sequence similarity 20, member A (FAM20A), transcript variant 1, Myc-DDK-tagged 10 ug
$804.00
RC215864L4 Lenti ORF clone of Human family with sequence similarity 20, member A (FAM20A), transcript variant 1, mGFP tagged 10 ug
$804.00
RG215864 FAM20A (tGFP-tagged) - Human family with sequence similarity 20, member A (FAM20A), transcript variant 1 10 ug
$489.00 $704.00
SC127400 FAM20A (untagged)-Human family with sequence similarity 20, member A (FAM20A), transcript variant 1 10 ug
$505.00

Plasmid Map for RC215864

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC215864
Circular map for RC215864

file-alt Citations

FAM20A binds to and regulates FAM20C localization
Ohyama, Lin, Govitvattana et al
Sci Rep (2016) 6, 27784 DOI: 10.1038/srep27784
Cited by 46
AI Summary: OriGene products contributed to this study by providing the plasmids containing the full-length sequences of human FAM20A and FAM20C, which were used as PCR templates for mutagenesis and expression experiments.
Snippet: mutagenesis with the megaprimer PCR procedure 41 . The plasmids containing the full length sequence of human FAM20A and FAM20C were purchased from OriGene and used as PCR templates. The primer sequences used in this ... Cite SaveSaving
FigureFigureFigure
Showing 1-1 of 1 papers.
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