SM22 alpha (TAGLN) (NM_003186) Human Tagged ORF Clone
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | SM22 alpha |
| Synonyms | SM22; SM22-alpha; SMCC; TAGLN1; WS3-10 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_003186 |
| ORF Size | 603 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_003186.5 |
| RefSeq Size | 1177 bp |
| RefSeq ORF | 606 bp |
| Locus ID | 6876 |
| UniProt ID | Q01995, Q5U0D2 |
| Cytogenetics | 11q23.3 |
| Domains | calponin, CH |
| MW | 22.4 kDa |
| Summary | This gene encodes a shape change and transformation sensitive actin-binding protein which belongs to the calponin family. It is ubiquitously expressed in vascular and visceral smooth muscle, and is an early marker of smooth muscle differentiation. The encoded protein is thought to be involved in calcium-independent smooth muscle contraction. It acts as a tumor suppressor, and the loss of its expression is an early event in cell transformation and the development of some tumors, coinciding with cellular plasticity. The encoded protein has a domain architecture consisting of an N-terminal calponin homology (CH) domain and a C-terminal calponin-like (CLIK) domain. Mice with a knockout of the orthologous gene are viable and fertile but their vascular smooth muscle cells exhibit alterations in the distribution of the actin filament and changes in cytoskeletal organization. provided by RefSeq, Aug 2017 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_003186" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC215789L1 | Lenti ORF clone of Human transgelin (TAGLN), transcript variant 2, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC215789L2 | Lenti ORF clone of Human transgelin (TAGLN), transcript variant 2, mGFP tagged | 10 ug |
$600.00
|
|
| RC215789L3 | Lenti ORF clone of Human transgelin (TAGLN), transcript variant 2, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC215789L4 | Lenti ORF clone of Human transgelin (TAGLN), transcript variant 2, mGFP tagged | 10 ug |
$600.00
|
|
| RG215789 | TAGLN (tGFP-tagged) - Human transgelin (TAGLN), transcript variant 2 | 10 ug |
$489.00
$500.00
|
|
| SC118118 | TAGLN (untagged)-Human transgelin (TAGLN), transcript variant 2 | 10 ug |
$300.00
|
|
Citations
GRASP55 regulates the unconventional secretion and aggregation of mutant huntingtin
Ahat, Bui, Zhang et al
J Biol Chem (2022) 298 (8), 102219 DOI: 10.1016/j.jbc.2022.102219
Ahat, Bui, Zhang et al
J Biol Chem (2022) 298 (8), 102219 DOI: 10.1016/j.jbc.2022.102219
Cited by 54
AI Summary: In this study, OriGene’s pCMV6-Htt-Q74 expression plasmid was used to express mutant huntingtin protein (Htt-Q74), enabling the investigation of GRASP55’s role in regulating the unconventional secretion and aggregation of this neurodegenerative disease-associated protein.
GRASP55 regulates the unconventional secretion and aggregation of mutant huntingtin
Ahat, Bui, Zhang et al
Journal of Biological Chemistry (2022) 298 (8), 102219 DOI: 10.1016/j.jbc.2022.102219
Ahat, Bui, Zhang et al
Journal of Biological Chemistry (2022) 298 (8), 102219 DOI: 10.1016/j.jbc.2022.102219
Cited by 54
AI Summary: In this study, the OriGene product TAGLN-Myc (catalog no.: RC215789) was used to investigate TAGLN as a novel cytosolic cargo secreted via the GRASP55-mediated unconventional protein secretion pathway.
Snippet: SP55 (WT, 37A, 5A), pEGFP-N1-GRASP65 (WT), and pEGFP-N1-Q80 (38, 64). Other complementary DNAs include GFP-Htt-Q74 (Addgene;catalog no.: 40262), GFP-Htt-Q23 (Addgene;catalog no.: 40261), TAGLN-Myc (OriGene;catalog no.: RC215789), PAICS-EGFP (Addgene;catalog no.: 99108), and pFRT/TO/HIS/FLAG/HA-PRDX1 (Addgene;catalog no.: 38086). Antibodies used in this study include monoclonal antibodies aga
Proteomic signatures corresponding to the SS18/SSX fusion gene in synovial sarcoma
Ishii, Suehara, Sano et al
Oncotarget (2018) 9 (101), 37509-37519 DOI: 10.18632/oncotarget.26493
Ishii, Suehara, Sano et al
Oncotarget (2018) 9 (101), 37509-37519 DOI: 10.18632/oncotarget.26493
Snippet: For retrovirus production, the pcx4 (PMID: 14597713) and pGEM (Promega) vector systems were used. A plasmid encoding human TAGLN (Origene) was used to generate constructs, which were subcloned into pGEM, and the construct were subcloned into pCX4bleo. Retroviruses were obtained using αφ cells as packaging cells and infected into 3 SS lines for selection with 500 μg/ml zeocin (Thermo Fisher Scienti...
Transgelin gene is frequently downregulated by promoter DNA hypermethylation in breast cancer
Sayar, Karahan, Konu et al
Clin Epigenetics (2015) 7, 104 DOI: 10.1186/s13148-015-0138-5
Sayar, Karahan, Konu et al
Clin Epigenetics (2015) 7, 104 DOI: 10.1186/s13148-015-0138-5
Cited by 51
AI Summary: OriGene products contributed to this study by providing the TAGLN cDNA clone (Cat# RC209875) and TAGLN shRNA plasmids (Cat# TG311438) used for overexpression and gene silencing experiments, respectively, to investigate the functional role of TAGLN in breast cancer.
Showing 1-4 of 4 papers.
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