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C3orf37 (HMCES) (NM_020187) Human Tagged ORF Clone

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SKU
RC215567
C3orf37 (Myc-DDK-tagged)-Human chromosome 3 open reading frame 37 (C3orf37), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol C3orf37
Synonyms C3orf37; DC12; SRAPD1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC215567 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGTGGGCGAACATCCTGTCACTTACCTAGAGATGTTCTCACGAGAGCTTGCGCCTACCAGGATCGGC
GGGGCCAGCAGCGGCTCCCGGAGTGGAGGGACCCTGATAAGTACTGCCCCTCTTACAACAAGAGTCCTCA
ATCCAACAGCCCAGTGCTTCTGTCTCGACTGCACTTTGAGAAGGATGCAGACTCATCTGAGCGTATCATT
GCTCCCATGCGCTGGGGCTTGGTCCCTTCTTGGTTCAAAGAAAGTGATCCTTCCAAGCTGCAGTTCAATA
CTACCAACTGTCGTAGTGATACCGTAATGGAGAAACGGTCATTTAAGGTGCCTCTGGGAAAGGGAAGACG
CTGTGTCGTTTTAGCAGATGGATTCTATGAGTGGCAGCGATGTCAGGGAACAAACCAGAGGCAGCCATAC
TTCATCTATTTTCCTCAAATCAAGACAGAGAAGTCAGGTAGCATTGGTGCTGCAGATAGTCCTGAGAACT
GGGAGAAAGTCTGGGACAACTGGAGGCTGCTGACAATGGCCGGGATCTTTGACTGCTGGGAGCCCCCAGA
GGGAGGAGATGTCCTGTATTCCTATACCATCATCACAGTGGATTCCTGCAAAGGCTTGAGTGACATCCAC
CACAGGATGCCTGCCATATTAGATGGAGAGGAGGCAGTTTCTAAATGGCTTGACTTTGGTGAAGTCTCAA
CTCAGGAAGCTCTGAAATTAATCCACCCAACAGAGAACATCACCTTCCATGCAGTCTCTTCTGTGGTGAA
CAACTCGCGAAACAACACTCCTGAGTGTCTGGCTCCTGTCGACTTGGTGGTCAAAAAGGAGCTCAGGGCA
AGTGGCAGTAGCCAGAGGATGTTGCAGTGGTTGGCCACAAAGTCACCCAAAAAGGAAGACTCAAAAACAC
CTCAAAAGGAAGAGTCAGATGTTCCCCAGTGGTCCAGTCAGTTCCTGCAGAAGAGTCCACTCCCCACCAA
GAGAGGCACTGCAGGACTCCTAGAGCAATGGCTGAAGCGGGAGAAGGAGGAGGAACCTGTGGCCAAGCGT
CCTTACAGCCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC215567 protein sequence
Red=Cloning site Green=Tags(s)

MCGRTSCHLPRDVLTRACAYQDRRGQQRLPEWRDPDKYCPSYNKSPQSNSPVLLSRLHFEKDADSSERII
APMRWGLVPSWFKESDPSKLQFNTTNCRSDTVMEKRSFKVPLGKGRRCVVLADGFYEWQRCQGTNQRQPY
FIYFPQIKTEKSGSIGAADSPENWEKVWDNWRLLTMAGIFDCWEPPEGGDVLYSYTIITVDSCKGLSDIH
HRMPAILDGEEAVSKWLDFGEVSTQEALKLIHPTENITFHAVSSVVNNSRNNTPECLAPVDLVVKKELRA
SGSSQRMLQWLATKSPKKEDSKTPQKEESDVPQWSSQFLQKSPLPTKRGTAGLLEQWLKREKEEEPVAKR
PYSQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020187
ORF Size 1062 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020187.3
RefSeq Size 1638 bp
RefSeq ORF 1065 bp
Locus ID 56941
UniProt ID Q96FZ2
Cytogenetics 3q21.3
Domains DUF159
MW 40.6 kDa
Summary Sensor of abasic sites in single-stranded DNA (ssDNA) required to preserve genome integrity by promoting error-free repair of abasic sites (PubMed:30554877). Acts as an enzyme that recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA (PubMed:30554877). The HMCES DNA-protein cross-link is then degraded by the proteasome (PubMed:30554877). Promotes error-free repair of abasic sites by acting as a 'suicide' enzyme that is degraded, thereby protecting abasic sites from translesion synthesis (TLS) polymerases and endonucleases that are error-prone and would generate mutations and double-strand breaks (PubMed:30554877). Acts as a protease: mediates autocatalytic processing of its N-terminal methionine in order to expose the catalytic cysteine (By similarity). Specifically binds 5-hydroxymethylcytosine (5hmC)-containing DNA in stem cells (By similarity). May act as an endonuclease that specifically cleaves 5hmC-containing DNA; additional experiments are however required to confirm this activity in vivo (By similarity).UniProtKB/Swiss-Prot Function
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"NM_020187" in other vectors (4)
SKU Description Size Price
RC215567L3 Lenti ORF clone of Human chromosome 3 open reading frame 37 (C3orf37), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC215567L4 Lenti ORF clone of Human chromosome 3 open reading frame 37 (C3orf37), transcript variant 2, mGFP tagged 10 ug
$757.00
RG215567 C3orf37 (tGFP-tagged) - Human chromosome 3 open reading frame 37 (C3orf37), transcript variant 2 10 ug
$489.00 $657.00
SC313096 C3orf37 (untagged)-Human chromosome 3 open reading frame 37 (C3orf37), transcript variant 2 10 ug
$503.00

Plasmid Map for RC215567

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC215567
Circular map for RC215567

file-alt Citations

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