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ARL5A (NM_012097) Human Tagged ORF Clone

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SKU
RC215504
ARL5A (Myc-DDK-tagged)-Human ADP-ribosylation factor-like 5A (ARL5A), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "ARL5A or NM_012097"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ARL5A
Synonyms ARFLP5; ARL5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC215504 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAATTCTCTTCACTAGAATATGGAGACTGTTCAATCACCAGGAGCACAAAGTTATCATTGTTGGGC
TGGATAATGCAGGGAAAACTACCATTCTTTACCAATTTTCTATGAATGAAGTTGTACATACATCTCCTAC
AATAGGAAGTAATGTAGAAGAGATAGTGATTAATAATACACGTTTCCTAATGTGGGATATTGGTGGCCAA
GAATCTCTTCGTTCTTCCTGGAACACTTACTATACTAACACAGAGTTTGTAATAGTTGTTGTGGACAGTA
CAGACAGAGAGAGGATTTCTGTAACTAGAGAAGAACTCTATAAAATGTTAGCGCATGAGGACCTAAGAAA
AGCTGGATTGCTGATTTTTGCTAATAAACAAGATGTTAAAGAATGCATGACTGTAGCAGAAATCTCCCAG
TTTTTGAAGCTAACTTCTATTAAAGATCACCAGTGGCATATCCAGGCATGCTGTGCTCTAACTGGCGAGG
GATTGTGCCAAGGACTTGAATGGATGATGTCACGACTTAAGATTAGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC215504 protein sequence
Red=Cloning site Green=Tags(s)

MGILFTRIWRLFNHQEHKVIIVGLDNAGKTTILYQFSMNEVVHTSPTIGSNVEEIVINNTRFLMWDIGGQ
ESLRSSWNTYYTNTEFVIVVVDSTDRERISVTREELYKMLAHEDLRKAGLLIFANKQDVKECMTVAEISQ
FLKLTSIKDHQWHIQACCALTGEGLCQGLEWMMSRLKIR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012097
ORF Size 537 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012097.4
RefSeq Size 3169 bp
RefSeq ORF 540 bp
Locus ID 26225
UniProt ID Q9Y689
Cytogenetics 2q23.3
Domains arf, ARF, SAR
MW 20.7 kDa
Summary The protein encoded by this gene belongs to the ARF family of GTP-binding proteins. With its distinctive nuclear/nucleolar localization and interaction with HP1alpha, the protein is developmentally regulated and may play a role(s) in nuclear dynamics and/or signaling cascades during embryonic development. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene has multiple pseudogenes. provided by RefSeq, Jul 2008
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"NM_012097" in other vectors (4)
SKU Description Size Price
RC215504L3 Lenti ORF clone of Human ADP-ribosylation factor-like 5A (ARL5A), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC215504L4 Lenti ORF clone of Human ADP-ribosylation factor-like 5A (ARL5A), transcript variant 1, mGFP tagged 10 ug
$600.00
RG215504 ARL5A (tGFP-tagged) - Human ADP-ribosylation factor-like 5A (ARL5A), transcript variant 1 10 ug
$489.00 $500.00
SC111231 ARL5A (untagged)-Human ADP-ribosylation factor-like 5A (ARL5A), transcript variant 1 10 ug
$300.00

Plasmid Map for RC215504

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC215504
Circular map for RC215504

file-alt Citations

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