gp340 (DMBT1) (NM_007329) Human Tagged ORF Clone
- TrueORF®
SKU
RC215454
DMBT1 (Myc-DDK-tagged)-Human deleted in malignant brain tumors 1 (DMBT1), transcript variant 2
$2,987.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | gp340 |
| Synonyms | GP340; muclin; SAG; SALSA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_007329 |
| ORF Size | 7239 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_007329.1, NP_015568.1 |
| RefSeq Size | 7686 bp |
| RefSeq ORF | 7242 bp |
| Locus ID | 1755 |
| UniProt ID | Q9UGM3 |
| Cytogenetics | 10q26.13 |
| Protein Families | Druggable Genome |
| MW | 260.76 kDa |
| Summary | Loss of sequences from human chromosome 10q has been associated with the progression of human cancers. This gene was originally isolated based on its deletion in a medulloblastoma cell line. This gene is expressed with transcripts of 6.0, 7.5, and 8.0 kb in fetal lung and with one transcript of 8.0 kb in adult lung, although the 7.5 kb transcript has not been characterized. The encoded protein precursor is a glycoprotein containing multiple scavenger receptor cysteine-rich (SRCR) domains separated by SRCR-interspersed domains (SID). Transcript variant 2 (8.0 kb) has been shown to bind surfactant protein D independently of carbohydrate recognition. This indicates that DMBT1 may not be a classical tumor suppressor gene, but rather play a role in the interaction of tumor cells and the immune system. provided by RefSeq, Mar 2016 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Citations
Squamous cell carcinoma subverts adjacent histologically normal epithelium to promote lateral invasion
Singh, Banerjee, Piao et al
J Exp Med (2021) 218 (6) DOI: 10.1084/jem.20200944
Singh, Banerjee, Piao et al
J Exp Med (2021) 218 (6) DOI: 10.1084/jem.20200944
Cited by 18
AI Summary: OriGene products contributed to this study by providing the DMBT1 cDNA clone (Cat# RC224095) and DMBT1 shRNA plasmids (Cat# TL314299), which were used to manipulate DMBT1 expression and investigate its role in squamous cell carcinoma invasion and metastasis.
Showing 1-1 of 1 papers.
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