LCE3B (NM_178433) Human Tagged ORF Clone

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SKU
RC215298
LCE3B (Myc-DDK-tagged)-Human late cornified envelope 3B (LCE3B)
  $150.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LCE3B
Synonyms LEP14
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC215298 representing NM_178433
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCTGCCAGCAGAACCAGCAGCAGTGCCAGCCTCTGCCCAAGTGCCCCTCACCCAAGTGCCCCCCAA
AGAGCTCAGCACAGTGTCTGCCTCCAGCCTCCTCCTGCTGTGCTCCAAGACCTGGGTGCTGTGGTGGCCC
CAGCTCTGAGGGCGGCTGCTGCCTGAGCCACCACAGGTGCTGCAGGTCCCACCGATGCCGGCGCCAGAGC
TCCAACTCCTGTGACAGAGGCAGTGGTCAGCAAGATGGTGCCTCCGACTGTGGCTATGGCTCTGGAGGCT
GCTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC215298 representing NM_178433
Red=Cloning site Green=Tags(s)

MSCQQNQQQCQPLPKCPSPKCPPKSSAQCLPPASSCCAPRPGCCGGPSSEGGCCLSHHRCCRSHRCRRQS
SNSCDRGSGQQDGASDCGYGSGGCC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178433
ORF Size 285 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178433.1, NP_848520.1
RefSeq Size 288 bp
RefSeq ORF 288 bp
Locus ID 353143
UniProt ID Q5TA77
Cytogenetics 1q21.3
MW 9.6 kDa
Summary A structural component of the cornified envelope of the stratum corneum involved in innate cutaneous host defense (Probable). Possesses defensin-like antimicrobial activity against a broad spectrum of Gram-positive and Gram-negative bacteria, both aerobic and anaerobic species. Upon inflammation, may regulate skin barrier repair by shaping cutaneous microbiota composition and immune response to bacterial antigens (PubMed:28634035).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_178433" in other vectors (4)
SKU Description Size Price
RC215298L3 Lenti ORF clone of Human late cornified envelope 3B (LCE3B), Myc-DDK-tagged 10 ug
$450.00
RC215298L4 Lenti ORF clone of Human late cornified envelope 3B (LCE3B), mGFP tagged 10 ug
$450.00
RG215298 LCE3B (tGFP-tagged) - Human late cornified envelope 3B (LCE3B) 10 ug
$350.00
SC307176 LCE3B (untagged)-Human late cornified envelope 3B (LCE3B) 10 ug
$165.00

Plasmid Map for RC215298

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Cloning scheme for RC215298

Circular map for RC215298

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