RILPL1 (NM_178314) Human Tagged ORF Clone

SKU
RC215279
RILPL1 (Myc-DDK-tagged)-Human Rab interacting lysosomal protein-like 1 (RILPL1)
  • TrueORF®
    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

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$732.00
3 Weeks*
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RILPL1
Synonyms GOSPEL; RLP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC215279 representing NM_178314
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAGGAGCGGGGGTCGGCGCTGGCGGCCGAGTCGGCGCTGGAGAAGAACGTGGCCGAGCTGACCG
TCATGGACGTGTACGACATCGCGTCGCTTGTGGGCCACGAGTTCGAGCGGGTCATTGACCAGCACGGCTG
CGAGGCCATCGCGCGCCTCATGCCCAAGGTCGTGCGCGTCCTGGAGATCCTGGAGGTGCTGGTCAGCCGC
CACCACGTCGCGCCCGAGCTGGACGAGCTGCGCCTGGAGCTGGACCGCCTGCGCCTGGAGAGGATGGACC
GCATCGAGAAGGAGCGCAAGCACCAGAAGGAGCTGGAGCTGGTGGAGGATGTGTGGCGAGGGGAGGCGCA
GGACCTCCTCTCCCAGATCGCCCAGCTGCAGGAGGAGAACAAGCAGCTCATGACCAACCTCTCCCACAAG
GATGTCAATTTCTCAGAGGAGGAGTTCCAGAAGCATGAAGGCATGTCAGAGCGGGAGCGACAGGTGATGA
AGAAGCTGAAGGAGGTGGTGGACAAACAACGCGACGAGATCCGCGCCAAGGACAGGGAGCTGGGCCTGAA
AAATGAGGACGTTGAGGCTTTACAGCAGCAGCAGACACGGCTGATGAAGATCAACCATGACCTTCGGCAC
CGGGTCACGGTGGTGGAGGCCCAGGGGAAAGCCCTGATCGAACAGAAGGTGGAGCTGGAGGCAGACCTGC
AGACCAAGGAGCAGGAGATGGGCAGCCTGCGAGCAGAGCTGGGGAAGTTGCGAGAGAGGCTGCAGGGGGA
GCACAGCCAGAATGGGGAGGAGGAGCCTGAGACGGAGCCGGTGGGAGAGGAGAGCATCTCCGACGCAGAG
AAGGTGGCCATGGATCTCAAGGACCCCAACCGCCCCCGGTTCACCCTGCAGGAGCTGCGGGACGTGCTGC
ACGAGAGGAACGAGCTCAAGTCCAAGGTGTTCTTGCTGCAGGAGGAGCTGGCTTACTATAAGAGTGAAGA
AATGGAAGAGGAAAACCGAATACCCCAACCCCCACCCATCGCCCACCCGAGGACGTCCCCCCAGCCGGAG
TCGGGCATCAAGCGACTGTTTAGCTTCTTCTCCCGAGATAAGAAGCGCCTGGCCAACACACAGAGAAACG
TGCACATCCAGGAGTCCTTTGGACAGTGGGCAAACACCCACCGCGATGACGGTTACACAGAGCAAGGACA
GGAAGCCCTGCAGCATCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC215279 representing NM_178314
Red=Cloning site Green=Tags(s)

MEEERGSALAAESALEKNVAELTVMDVYDIASLVGHEFERVIDQHGCEAIARLMPKVVRVLEILEVLVSR
HHVAPELDELRLELDRLRLERMDRIEKERKHQKELELVEDVWRGEAQDLLSQIAQLQEENKQLMTNLSHK
DVNFSEEEFQKHEGMSERERQVMKKLKEVVDKQRDEIRAKDRELGLKNEDVEALQQQQTRLMKINHDLRH
RVTVVEAQGKALIEQKVELEADLQTKEQEMGSLRAELGKLRERLQGEHSQNGEEEPETEPVGEESISDAE
KVAMDLKDPNRPRFTLQELRDVLHERNELKSKVFLLQEELAYYKSEEMEEENRIPQPPPIAHPRTSPQPE
SGIKRLFSFFSRDKKRLANTQRNVHIQESFGQWANTHRDDGYTEQGQEALQHL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_178314
ORF Size 1209 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178314.5
RefSeq Size 2624 bp
RefSeq ORF 1212 bp
Locus ID 353116
UniProt ID Q5EBL4
Cytogenetics 12q24.31
MW 47.1 kDa
Summary Plays a role in the regulation of cell shape and polarity. Plays a role in cellular protein transport, including protein transport away from primary cilia. Neuroprotective protein, which acts by sequestring GAPDH in the cytosol and prevent the apoptotic function of GAPDH in the nucleus. Competes with SIAH1 for binding GAPDH (By similarity). Does not regulate lysosomal morphology and distribution.[UniProtKB/Swiss-Prot Function]
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SKU Description Size Price
RG215279 RILPL1 (tGFP-tagged) - Human Rab interacting lysosomal protein-like 1 (RILPL1) 10 ug
$932.00
SC309259 RILPL1 (untagged)-Human Rab interacting lysosomal protein-like 1 (RILPL1) 10 ug
$732.00
SC327829 RILPL1 (untagged)-Human Rab interacting lysosomal protein-like 1 (RILPL1) 10 ug
$732.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.