KRTAP10-9 (NM_198690) Human Tagged ORF Clone

  • TrueORF®
SKU
RC215254
KRTAP10 (Myc-DDK-tagged)-Human keratin associated protein 10-9 (KRTAP10-9). Note: ORF is codon optimized
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol KRTAP10-9
Synonyms KAP10.9; KAP18.9; KRTAP18-9
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC215254 ORF sequence, codon optimized.
Due to the complexity of NM_198690, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical
.

Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCTTCAACAATGTCCATCCGCTCATCTGCTTACAGCGATTCATGGCAGGTGGATGATTGTCCTG
AGAGCTGTTGCGAGCCCCCGTGCTGCGCTACCTCCTGCTGTGCCCCGGCTCCCTGTCTCACCCTGGTCTG
TACACCAGTCTCTAGAGTGTCTAGTCCCTGCTGTCAGGTGACGTGCGAACCAAGCCCATGCCAGTCAGGT
TGTACCAGCTCCTGCACTCCTTCTTGCTGCCAGCAATCCAGCTGCCAGCCCGCCTACTGTACCTCTTCCC
CCTGCCAGCAAGCCTGCTGCGTTCCAGTTTGCTGTAAACCTGTGTGCTGTGTCCCTGTGTGTTGTGGAGC
GAGCTCATGTTGTCAACAATCCTCTTACCAACCTGCTTGCTGTGCCTCCAGTAGTTGTCAGCCCGCATGC
TGTGTGCCAGTCTGCTGCAAGCCGGTATGTTGCGCGCCTACATGCTCCGAAGATAGTTACAGCTGTTGCC
AGCAGTCCAGTTGTCAACCCGCTTGCTGCACCAGCTCTCCATGTCAACAGTCTTACTGTGTGCCGGTGTG
CTGTAAGCCAGTGTGTTGTAAACCCATCTGTTGCGTACCCGTTTGTAGCGGCGCGAGCTCACTGTGTTGC
CAACAGTCTGGATGCCAGCCTGCATGCTGCACTACCAGTTGTTGTCGGCCATCCAGCTCCGTGTCCCTGC
TTTGTCGGCCAGTTTGTCGACCCGCCTGTTGCGTCCCCGTGTCCTCTTGCTGCGCACCCACTAGCTCTAG
GCAACCCAGCTACTGCCGGCAGGCGAGCTGCGTTAGTCTGCTCTGTCGGCCCGTTTGTTCTCGACCAGCC
TGTTACAGTTTTAGTAGCGGGCAGAAGTCATCTTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC215254 representing NM_198690
Red=Cloning site Green=Tags(s)

MAASTMSIRSSAYSDSWQVDDCPESCCEPPCCATSCCAPAPCLTLVCTPVSRVSSPCCQVTCEPSPCQSG
CTSSCTPSCCQQSSCQPAYCTSSPCQQACCVPVCCKPVCCVPVCCGASSCCQQSSYQPACCASSSCQPAC
CVPVCCKPVCCAPTCSEDSYSCCQQSSCQPACCTSSPCQQSYCVPVCCKPVCCKPICCVPVCSGASSLCC
QQSGCQPACCTTSCCRPSSSVSLLCRPVCRPACCVPVSSCCAPTSSRQPSYCRQASCVSLLCRPVCSRPA
CYSFSSGQKSSC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_198690
ORF Size 876 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_198690.1, NM_198690.2, NP_941963.1, NP_941963.2
RefSeq Size 1276 bp
RefSeq ORF 879 bp
Locus ID 386676
UniProt ID P60411
Cytogenetics 21q22.3
MW 30 kDa
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Documents
Resources
"NM_198690" in other vectors (2)
SKU Description Size Price
RG215254 KRTAP10 (tGFP-tagged) - Human keratin associated protein 10-9 (KRTAP10-9). Note: ORF is codon optimized 10 ug
$500.00
SC307769 KRTAP10 (untagged)-Human keratin associated protein 10-9 (KRTAP10-9) 10 ug
$330.00

Plasmid Map for RC215254

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Cloning scheme for RC215254

Circular map for RC215254

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