PRODH2 (NM_021232) Human Tagged ORF Clone
$748.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PRODH2 |
| Synonyms | HSPOX1; HYPDH |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_021232 |
| ORF Size | 1608 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_021232.1, NP_067055.1 |
| RefSeq Size | 1677 bp |
| RefSeq ORF | 1383 bp |
| Locus ID | 58510 |
| UniProt ID | Q9UF12 |
| Cytogenetics | 19q13.12 |
| Protein Pathways | Arginine and proline metabolism, Metabolic pathways |
| MW | 58.7 kDa |
| Summary | The protein encoded by this gene catalyzes the first step in the catabolism of trans-4-hydroxy-L-proline, an amino acid derivative obtained through food intake and collagen turnover. One of the downstream products of this catabolism is glyoxylate, which in people with disorders of glyoxalate metabolism can lead to an increase in oxalate levels and the formation of calcium-oxalate kidney stones. Therefore, this gene may serve as a therapeutic target against primary hyperoxalurias (PH). This gene is similar to proline dehydrogenase (oxidase) 1, a mitochondrial enzyme that catalyzes the first step in proline catabolism. provided by RefSeq, Jan 2017 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_021232" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC215144L1 | Lenti ORF clone of Human proline dehydrogenase (oxidase) 2 (PRODH2), Myc-DDK-tagged | 10 ug |
$1,048.00
|
|
| RC215144L2 | Lenti ORF clone of Human proline dehydrogenase (oxidase) 2 (PRODH2), mGFP tagged | 10 ug |
$1,048.00
|
|
| RC215144L3 | Lenti ORF clone of Human proline dehydrogenase (oxidase) 2 (PRODH2), Myc-DDK-tagged | 10 ug |
$1,048.00
|
|
| RC215144L4 | Lenti ORF clone of Human proline dehydrogenase (oxidase) 2 (PRODH2), mGFP tagged | 10 ug |
$1,048.00
|
|
| RG215144 | PRODH2 (tGFP-tagged) - Human proline dehydrogenase (oxidase) 2 (PRODH2) | 10 ug |
$948.00
|
|
| SC304927 | PRODH2 (untagged)-Human proline dehydrogenase (oxidase) 2 (PRODH2) | 10 ug |
$750.00
|
|
Citations
Proline dehydrogenase 2 (PRODH2) is a hydroxyproline dehydrogenase (HYPDH) and molecular target for treating primary hyperoxaluria
Summitt, Johnson, Jönsson et al
Biochem. J. (2015) 466 (2), 273-81 DOI: 10.1042/BJ20141159
Summitt, Johnson, Jönsson et al
Biochem. J. (2015) 466 (2), 273-81 DOI: 10.1042/BJ20141159
AI Summary: OriGene contributed to this study by providing the full-length PRODH2 gene, which was used for cloning and subsequent biochemical characterization of the enzyme.
Snippet: in PH patients. EXPERIMENTAL. Cloning. The full-length PRODH2 gene was obtained from OriGene and subcloned into the pET19b vector (Novagen) between the NdeI and BamHI restriction sites. This engineered construct ... Cite SaveSaving
Showing 1-1 of 1 papers.
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