UBE3C (NM_014671) Human Tagged ORF Clone
$943.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | UBE3C |
| Synonyms | HECTH2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_014671 |
| ORF Size | 3249 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_014671.3 |
| RefSeq Size | 5160 bp |
| RefSeq ORF | 3252 bp |
| Locus ID | 9690 |
| UniProt ID | Q15386 |
| Cytogenetics | 7q36.3 |
| Domains | HECT, IQ |
| Protein Families | Druggable Genome |
| Protein Pathways | Ubiquitin mediated proteolysis |
| MW | 123.7 kDa |
| Summary | E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_014671" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC215110L1 | Lenti ORF clone of Human ubiquitin protein ligase E3C (UBE3C), Myc-DDK-tagged | 10 ug |
$1,243.00
|
|
| RC215110L2 | Lenti ORF clone of Human ubiquitin protein ligase E3C (UBE3C), mGFP tagged | 10 ug |
$1,243.00
|
|
| RC215110L3 | Lenti ORF clone of Human ubiquitin protein ligase E3C (UBE3C), Myc-DDK-tagged | 10 ug |
$1,243.00
|
|
| RC215110L4 | Lenti ORF clone of Human ubiquitin protein ligase E3C (UBE3C), mGFP tagged | 10 ug |
$1,243.00
|
|
| RG215110 | UBE3C (tGFP-tagged) - Human ubiquitin protein ligase E3C (UBE3C) | 10 ug |
$789.00
$1,143.00
|
|
| SC315161 | UBE3C (untagged)-Human ubiquitin protein ligase E3C (UBE3C) | 10 ug |
$944.00
|
|
Citations
Identification of UBE3C as an E3 ubiquitin ligase for mutant BRAF
Kim, Yun, You et al
Life Sci (2025)
Kim, Yun, You et al
Life Sci (2025)
Cited by 2
Novel gene-intergenic fusion involving ubiquitin E3 ligase UBE3C causes distal hereditary motor neuropathy
Cutrupi, Narayanan, Perez-Siles et al
Brain (2023) 146 (3), 880-897 DOI: 10.1093/brain/awac424
Cutrupi, Narayanan, Perez-Siles et al
Brain (2023) 146 (3), 880-897 DOI: 10.1093/brain/awac424
Cited by 14
AI Summary: OriGene's pCMV6-Entry-UBE3C plasmid (Cat# RC215110) was used as the template to generate a mutant construct expressing the novel UBE3C-intergenic fusion, enabling functional studies of its role in distal hereditary motor neuropathy.
Snippet: Q5 site-directed mutagenesis (SDM;New England Biolabs) was used to generate the pCMV6-Entry-UBE3C-IF plasmid. This was conducted in two stages. (i) The commercial template plasmid pCMV6-Entry-UBE3C (Origene #: RC215110) was amplified using primers which partially inserted the intergenic fusion sequence and simultaneously deleted the UBE3C coding sequence downstream of p.Val443 (F: 5′-aagaggatca
Tegument protein UL21 of alpha-herpesvirus inhibits the innate immunity by triggering CGAS degradation through TOLLIP-mediated selective autophagy
Ma, Bai, Jiang et al
Autophagy (2022)
Ma, Bai, Jiang et al
Autophagy (2022)
Cited by 102
AI Summary: OriGene products Flag-CALCOCO2 (RC203843), Flag-BNIP3L (RC203315), and Flag-TOLLIP (RC200227) were used as expression plasmids to study the roles of autophagy cargo receptors in the mechanism by which alpha-herpesvirus UL21 protein induces CGAS degradation and suppresses innate immunity.
Showing 1-3 of 3 papers.
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