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Striatin (STRN) (NM_003162) Human Tagged ORF Clone

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SKU
RC215098
STRN (Myc-DDK-tagged)-Human striatin, calmodulin binding protein (STRN)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $714.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Striatin
Synonyms PPP2R6A; SG2NA; STRN1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC215098 representing NM_003162
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGAGCAGGCGGGTCCCGGCGTCTTCTTCAGCAACAACCACCCGGGCGCCGGCGGTGCCAAGGGGC
TCGGGCCTCTGGCGGAGGCTGCCGCGGCCGGCGACGGGGCGGCTGCGGCGGGGGCGGCCCGAGCCCAGTA
CAGTCTCCCGGGGATCCTGCACTTCCTGCAGCACGAGTGGGCCCGCTTCGAGGTGGAGAGAGCCCAGTGG
GAGGTGGAGCGGGCGGAGCTGCAGGCCCAGATTGCCTTCCTGCAGGGAGAAAGGAAGGGCCAAGAAAATT
TGAAGAAGGATCTTGTGAGGAGGATCAAAATGTTGGAGTATGCTCTTAAACAGGAAAGAGCCAAATACCA
CAAGTTGAAATACGGGACAGAATTGAATCAGGGAGATATGAAGCCTCCAAGCTATGATTCTGATGAAGGT
AATGAAACAGAAGTGCAGCCACAACAAAACAGCCAGTTAATGTGGAAACAAGGTCGACAACTACTCAGAC
AGTATCTACAGGAGGTGGGTTATACAGATACTATTCTAGATGTGAAATCTAAACGAGTGCGAGCTTTGTT
GGGCTTTTCAAGTGATGTCACGGACAGGGAAGATGACAAAAATCAGGACTCAGTTGTAAATGGCACAGAG
GCTGAAGTTAAAGAGACAGCAATGATTGCAAAATCTGAGTTAACAGATTCTGCCTCCGTGCTGGATAATT
TCAAATTCCTTGAAAGTGCAGCTGCAGATTTCAGTGATGAAGATGAAGATGATGATGTTGATGGAAGAGA
GAAAAGCGTCATTGATACTTCAACAATTGTTAGGAAAAAAGCATTGCCTGACAGCGGTGAAGATCGAGAT
ACAAAAGAAGCTCTAAAGGAGTTTGACTTCTTGGTTACATCAGAGGAAGGAGACAATGAATCTAGAAGTG
CAGGCGATGGAACAGACTGGGAAAAGGAAGACCAGTGTCTCATGCCTGAAGCCTGGAATGTGGACCAGGG
AGTAATTACCAAACTCAAGGAACAATACAAAAAGGAGAGAAAGGGGAAAAAGGGGGTGAAGAGGCCCAAT
AGGTCAAAACTACAAGATATGCTTGCTAATTTGAGAGATGTTGATGAACTTCCTTCATTGCAGCCATCTG
TGGGTTCACCTTCCAGACCCAGCAGCTCCAGGCTTCCTGAACATGAAATTAATAGGGCAGATGAAGTGGA
AGCATTGACATTTCCTCCTTCTTCTGGAAAGTCATTCATCATGGGAGCAGATGAAGCCCTTGAAAGTGAA
CTGGGACTTGGAGAACTAGCAGGCCTTACGGTGGCCAATGAAGCAGACTCACTAACTTATGATATAGCAA
ACAATAAAGATGCATTGAGGAAGACATGGAACCCTAAGTTTACATTGAGAAGTCACTTTGATGGCATCCG
AGCCCTTGCTTTCCATCCCATTGAGCCTGTTTTGATAACAGCATCAGAGGATCACACATTAAAAATGTGG
AATTTACAGAAAACAGCCCCAGCCAAAAAGAGCACTTCTCTTGATGTAGAACCTATCTATACATTCAGAG
CCCATAAAGGTCCAGTGCTTTGTGTGGTAATGAGCAGCAATGGTGAGCAGTGTTACAGTGGTGGTACTGA
TGGACTGATCCAGGGCTGGAATACCACTAATCCCAACATCGACCCCTATGATTCTTATGATCCTTCTGTT
TTACGAGGCCCTCTGCTAGGCCACACGGATGCAGTCTGGGGTTTGGCTTATAGTGCAGCACATCAGCGTT
TGTTGTCCTGTTCAGCAGATGGCACTCTGCGTTTATGGAATACAACTGAGGTTGCTCCAGCACTAAGTGT
ATTTAATGATACTAAAGAACTGGGAATCCCTGCCTCTGTGGATCTAGTGAGCAGTGACCCGAGCCATATG
GTAGCATCATTCAGCAAGGGATATACAAGCATTTTTAACATGGAAACACAACAACGCATTCTCACTTTAG
AATCCAATGTAGATACAACAGCCAACTCTTCCTGCCAAATAAATAGAGTCATCAGTCATCCTACTCTTCC
GATCAGCATCACTGCTCATGAAGACAGGCACATCAAATTCTATGATAACAATACAGGCAAACTGATCCAC
TCGATGGTAGCCCACCTAGAAGCTGTTACAAGTTTAGCAGTTGATCCCAATGGCCTTTACTTGATGTCTG
GCAGTCATGACTGTTCAATACGTTTATGGAATCTAGAAAGTAAGACGTGTATCCAAGAATTCACAGCTCA
TCGAAAAAAGTTTGAAGAATCGATTCATGATGTAGCTTTCCACCCATCCAAATGCTATATAGCCAGTGCT
GGAGCTGACGCACTGGCTAAAGTCTTTGTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC215098 representing NM_003162
Red=Cloning site Green=Tags(s)

MDEQAGPGVFFSNNHPGAGGAKGLGPLAEAAAAGDGAAAAGAARAQYSLPGILHFLQHEWARFEVERAQW
EVERAELQAQIAFLQGERKGQENLKKDLVRRIKMLEYALKQERAKYHKLKYGTELNQGDMKPPSYDSDEG
NETEVQPQQNSQLMWKQGRQLLRQYLQEVGYTDTILDVKSKRVRALLGFSSDVTDREDDKNQDSVVNGTE
AEVKETAMIAKSELTDSASVLDNFKFLESAAADFSDEDEDDDVDGREKSVIDTSTIVRKKALPDSGEDRD
TKEALKEFDFLVTSEEGDNESRSAGDGTDWEKEDQCLMPEAWNVDQGVITKLKEQYKKERKGKKGVKRPN
RSKLQDMLANLRDVDELPSLQPSVGSPSRPSSSRLPEHEINRADEVEALTFPPSSGKSFIMGADEALESE
LGLGELAGLTVANEADSLTYDIANNKDALRKTWNPKFTLRSHFDGIRALAFHPIEPVLITASEDHTLKMW
NLQKTAPAKKSTSLDVEPIYTFRAHKGPVLCVVMSSNGEQCYSGGTDGLIQGWNTTNPNIDPYDSYDPSV
LRGPLLGHTDAVWGLAYSAAHQRLLSCSADGTLRLWNTTEVAPALSVFNDTKELGIPASVDLVSSDPSHM
VASFSKGYTSIFNMETQQRILTLESNVDTTANSSCQINRVISHPTLPISITAHEDRHIKFYDNNTGKLIH
SMVAHLEAVTSLAVDPNGLYLMSGSHDCSIRLWNLESKTCIQEFTAHRKKFEESIHDVAFHPSKCYIASA
GADALAKVFV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003162
ORF Size 2340 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003162.4
RefSeq Size 3479 bp
RefSeq ORF 2343 bp
Locus ID 6801
UniProt ID O43815
Cytogenetics 2p22.2
Domains WD40
MW 86 kDa
Summary Calmodulin-binding protein which may function as scaffolding or signaling protein and may play a role in dendritic Ca(2+) signaling.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_003162" in other vectors (6)
SKU Description Size Price
RC215098L1 Lenti ORF clone of Human striatin, calmodulin binding protein (STRN), Myc-DDK-tagged 10 ug
$1,014.00
RC215098L2 Lenti ORF clone of Human striatin, calmodulin binding protein (STRN), mGFP tagged 10 ug
$1,014.00
RC215098L3 Lenti ORF clone of Human striatin, calmodulin binding protein (STRN), Myc-DDK-tagged 10 ug
$1,014.00
RC215098L4 Lenti ORF clone of Human striatin, calmodulin binding protein (STRN), mGFP tagged 10 ug
$1,014.00
RG215098 STRN (tGFP-tagged) - Human striatin, calmodulin binding protein (STRN) 10 ug
$789.00 $914.00
SC118143 STRN (untagged)-Human striatin, calmodulin binding protein (STRN) 10 ug
$715.00

Plasmid Map for RC215098

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC215098
Circular map for RC215098

file-alt Citations

New age-related hearing loss candidate genes in humans: an ongoing challenge
Di Stazio, Morgan, Brumat et al
Gene (2020) 742, 144561 DOI: 10.1016/j.gene.2020.144561
Cited by 15
AI Summary: OriGene’s pCMV6-Entry vectors containing wild-type and mutant cDNAs of candidate genes (Slc44a2, Slc28a3, Cep104, Dclk1, Pcdh20, Sik3, and Strn) were used for transfection and protein expression studies to functionally assess gene variants associated with age-related hearing loss in this paper.
Snippet: The WT and human mutant genes of interest (Slc44a2, Slc28a3, Cep104, Dclk1, Pcdh20, Sik3 and Strn) cDNAs were cloned into a pCMV6-Entry vector (by Origene, Rockville, MD), Myc tagged, respectively. Vectors were purified through the PureLink HiPure Plasmid Maxiprep kit (Invitrogen) for transfection and protein expression
Showing 1-1 of 1 papers.
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