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SPAG16 (NM_001025436) Human Tagged ORF Clone

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SKU
RC215059
SPAG16 (Myc-DDK-tagged)-Human sperm associated antigen 16 (SPAG16), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "SPAG16 or NM_001025436"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SPAG16
Synonyms PF20; WDR29
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC215059 representing NM_001025436
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCTCAGCGAGGGATGCCCAGCTCCGCCGTGAGGGTCCTGGAAGAGGCGTTGGGCATGGGTTTGA
CGGCAGCCGGGGACGCGAGGGACACGGCGGACGCGGTGGCGGCTGAGGGCGCCTACTACCTGGAACAGGT
CACCATAACTGAAGCATCTGAAGATGACTATGAATATGAAGAGATACCAGATGACAATTTTAGCATCCCA
GAAGGTGAAGAAGATCTGGCAAAAGCAATTCAGATGGCCCAAGAACAGGCTACAGATACTGAAATTTTGG
AACGGAAAACAGTTCTTCCTTCAAAGCATGCAGTACCTGAAGTAATAGAAGACTTTCTCTGCAATTTCTT
GATCAAAATGGGAATGACCAGAACTCTTGATTGCTTTCAGTCTGAATGGTATGAGTTAATACAGAAAGGA
GTGACTGAACTTAGAACTGTTGGGAATGTTCCAGATGTCTACACCCAGATTATGCTTTTGGAAAATGAGA
ACAAAAATTTAAAGAAAGATTTGAAGCACTACAAACAAGCAGCTGAGTATGTTATTTTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC215059 representing NM_001025436
Red=Cloning site Green=Tags(s)

MAAQRGMPSSAVRVLEEALGMGLTAAGDARDTADAVAAEGAYYLEQVTITEASEDDYEYEEIPDDNFSIP
EGEEDLAKAIQMAQEQATDTEILERKTVLPSKHAVPEVIEDFLCNFLIKMGMTRTLDCFQSEWYELIQKG
VTELRTVGNVPDVYTQIMLLENENKNLKKDLKHYKQAAEYVIF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001025436
ORF Size 549 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001025436.2, NP_001020607.1
RefSeq Size 1296 bp
RefSeq ORF 552 bp
Locus ID 79582
UniProt ID Q8N0X2, Q4G1A2
Cytogenetics 2q34
MW 20.4 kDa
Summary Cilia and flagella are comprised of a microtubular backbone, the axoneme, which is organized by the basal body and surrounded by plasma membrane. SPAG16 encodes 2 major proteins that associate with the axoneme of sperm tail and the nucleus of postmeiotic germ cells, respectively (Zhang et al., 2007 PubMed 17699735).supplied by OMIM, Jul 2008
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"NM_001025436" in other vectors (4)
SKU Description Size Price
RC215059L3 Lenti ORF clone of Human sperm associated antigen 16 (SPAG16), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC215059L4 Lenti ORF clone of Human sperm associated antigen 16 (SPAG16), transcript variant 2, mGFP tagged 10 ug
$600.00
RG215059 SPAG16 (tGFP-tagged) - Human sperm associated antigen 16 (SPAG16), transcript variant 2 10 ug
$500.00
SC302404 SPAG16 (untagged)-Human sperm associated antigen 16 (SPAG16), transcript variant 2 10 ug
$330.00

Plasmid Map for RC215059

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC215059
Circular map for RC215059

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