hSET1 (SETD1A) (NM_014712) Human Tagged ORF Clone
$1,434.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | hSET1 |
| Synonyms | EPEDD; KMT2F; NEDSID; Set1; Set1A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_014712 |
| ORF Size | 5121 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_014712.3 |
| RefSeq Size | 6447 bp |
| RefSeq ORF | 5124 bp |
| Locus ID | 9739 |
| UniProt ID | O15047 |
| Cytogenetics | 16p11.2 |
| Protein Families | Druggable Genome |
| Protein Pathways | Lysine degradation |
| MW | 185.9 kDa |
| Summary | The protein encoded by this gene is a component of a histone methyltransferase (HMT) complex that produces mono-, di-, and trimethylated histone H3 at Lys4. Trimethylation of histone H3 at lysine 4 (H3K4me3) is a chromatin modification known to generally mark the transcription start sites of active genes. The protein contains SET domains, a RNA recognition motif domain and is a member of the class V-like SAM-binding methyltransferase superfamily. provided by RefSeq, Dec 2016 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_014712" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC214996L1 | Lenti ORF clone of Human SET domain containing 1A (SETD1A), Myc-DDK-tagged | 10 ug |
$1,734.00
|
|
| RC214996L3 | Lenti ORF clone of Human SET domain containing 1A (SETD1A), Myc-DDK-tagged | 10 ug |
$1,734.00
|
|
| RG214996 | SETD1A (tGFP-tagged) - Human SET domain containing 1A (SETD1A) | 10 ug |
$1,634.00
|
|
| SC304157 | SETD1A (untagged)-Human SET domain containing 1A (SETD1A) | 10 ug |
$1,579.00
|
|
Citations
SETD1A regulates transcriptional pause release of heme biosynthesis genes in leukemia
Hoshii, Perlee, Kikuchi et al
Cell Rep (2022) 41 (9), 111727 DOI: 10.1016/j.celrep.2022.111727
Hoshii, Perlee, Kikuchi et al
Cell Rep (2022) 41 (9), 111727 DOI: 10.1016/j.celrep.2022.111727
Cited by 18
AI Summary: OriGene’s pCMV6-hSETD1A(NM_014712)-Myc-DDK plasmid (Cat# RC214996) was used in this study to investigate the role of SETD1A in regulating transcriptional pause release of heme biosynthesis genes in leukemia cells.
Snippet: Addgene #57824 Plasmid: pMD2.G Laboratory of Didier Trono Addgene #12259 Plasmid: psPAX2 Laboratory of Didier Trono Addgene #12260 Plasmid: pCMV6-hSETD1A(NM_014712)-Myc-DDK Origene Cat#RC214996 Plasmid: pMXs-3XHA-EGFP-OMP25 Chen et al. 201645 Addgene #83356 ------------------------- Software and algorithms ------------------------- Prism 9 Graph
A Non-catalytic Function of SETD1A Regulates Cyclin K and the DNA Damage Response
Hoshii, Cifani, Feng et al
Cell (2018) 172 (5), 1007-1021.e17 DOI: 10.1016/j.cell.2018.01.032
Hoshii, Cifani, Feng et al
Cell (2018) 172 (5), 1007-1021.e17 DOI: 10.1016/j.cell.2018.01.032
AI Summary: OriGene products pCMV6-hSETD1A (Cat# RC214996) and pCMV6-hSETD1B (Cat# RC227091) plasmids were used to express SETD1A and SETD1B proteins, enabling functional studies that revealed the essential, non-catalytic role of SETD1A in regulating cyclin K and the DNA damage response in acute myeloid leukemia cells.
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
