JMJD1C (NM_032776) Human Tagged ORF Clone
- TrueORF®
SKU
RC214878
JMJD1C (Myc-DDK-tagged)-Human jumonji domain containing 1C (JMJD1C), transcript variant 1
$2,096.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | JMJD1C |
| Synonyms | KDM3C; TRIP-8; TRIP8 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_032776 |
| ORF Size | 7620 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_032776.2, NP_116165.1 |
| RefSeq Size | 8786 bp |
| RefSeq ORF | 7623 bp |
| Locus ID | 221037 |
| UniProt ID | Q15652 |
| Cytogenetics | 10q21.3 |
| Protein Families | Druggable Genome |
| MW | 285 kDa |
| Summary | The protein encoded by this gene interacts with thyroid hormone receptors and contains a jumonji domain. It is a candidate histone demethylase and is thought to be a coactivator for key transcription factors. It plays a role in the DNA-damage response pathway by demethylating the mediator of DNA damage checkpoint 1 (MDC1) protein, and is required for the survival of acute myeloid leukemia. Mutations in this gene are associated with Rett syndrome and intellectual disability. Alternative splicing results in multiple transcript variants. provided by RefSeq, Dec 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Citations
Histone demethylase JMJD1C is phosphorylated by mTOR to activate de novo lipogenesis
Viscarra, Wang, Nguyen et al
Nat Commun (2020) 11 (1), 796 DOI: 10.1038/s41467-020-14617-1
Viscarra, Wang, Nguyen et al
Nat Commun (2020) 11 (1), 796 DOI: 10.1038/s41467-020-14617-1
Cited by 75
AI Summary: OriGene contributed to this study by providing the full-length human JMJD1C plasmid, which was used to investigate the role of JMJD1C in lipogenic gene transcription.
Snippet: H3K9me2 (ab176882, 2 µg/µg of chromatin), H3K9me3 (ab8898, 2 µg/µg of chromatin), and H3K4me3 (ab8580, 2 µg/µg of chromatin) from Abcam. Full-length human JMJD1C plasmid was obtained from OriGene. HepG2 and 293FT cells were obtained from ATCC. Cells were grown in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum and 100 units/mL of penicillin and strep
Showing 1-1 of 1 papers.
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