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AGAP8 (NM_001077686) Human Tagged ORF Clone

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SKU
RC214807
AGAP8 (Myc-DDK-tagged)-Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 8 (AGAP8)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $617.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol AGAP8
Synonyms AGAP-8; CTGLF5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214807 representing NM_001077686
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGAACATACTGACCTGTCGTGTGCACCCTAGCGTCAGCCTCGAGTTTGACCAGCAGCAGGGGTCGG
TGTGTCCCTCTGAATCTGAGATCTATGAGGCAGGAGCTGGGGACAGGATGGCAGGAGCGCCCATGGCTGC
TGCTGTACAGCCTGCTGAGGTGACTGTTGAAGTTGGTGAGGACCTCCACATGCACCACGTTCGTGACCGG
GAGATGCCTGAAGCTTTGGAGTTTAACCCTTCTGCCAATCCAGAGGCAAGCACAATATTCCAGAGGAACT
CTCAAACAGATGTTGTAGAAATAAGAAGAAGCAACTGTACAAACCATGTATCTACTGTGCGTTTCAGTCA
ACAATACAGCTTGTGTTCGACAATATTCCTTGATGACAGCACAGCCATCCAGCATTATCTTACAATGACA
ATAATATCTGTGACCTTGGAGATACCTCATCATATCACACAAAGAGATGCAGATAGATCTTTGAGCATAC
CTGATGAACAGTTACACTCATTTGCGGTTTCCACCGTGCACATTATGAAGAAAAGAAATGGAGGTGGGAG
TTTAAATAACTATTCCTCCTCCATTCCATCGACTCCCAGCACCAGCCAGGAGGACCCTCAGTTCAGTGTT
CCTCCCACTGCCAACACACCCACCCCCGTTTGCAAGCGGTCCATGCGCTGGTCCAACCTGTTTACATCTG
AGAAAGGGAGTGACCCAGACAAAGAGAGGAAAGCCCCGGAGAATCATGCTGACACCATCGGGAGCGGCAG
AGCCATCCCCATTAAACAGGGCATGCTCTTAAAGCGAAGTGGGAAATGGCTGAAGACATGGAAAAAGAAA
TACGTCACCCTGTGTTCCAATGGCGTGCTCACCTATTATTCAAGCTTAGGTGATTATATGAAGAATATTC
ATAAAAAAGAGATTGACCTTCAGACATCTACCATCAAAGTCCCAGGAAAGTGGCCATCCCTAGCCACATC
GGCCTGCACACCCATCTCCAGCTCTAAAAGCAATGGCCTATCCAAGGACATGGACACCGGGCTGGGTGAC
TCCATATGCTTCAGCCCCAGTATCTCCAGCACCACCAGCCCCAAGCTCAACCCGCCCCCCTCTCCTCATG
CCAATAAAAAGAAACACCTAAAGAAGAAAAGCACCAACAACTTTATGATTGTGTCTGCCACTGGCCAAAC
GTGGCACTTTGAAGCCACGACATATGAGGAGCGGGATGCCTGGGTCCAAGCCATCCAGAGCCAGATCCTG
GCCAGCCTGCAGTCATGCGAGAGCAGTAAAAGCAAGTCCCAGCTGACCAGCCAGAGCGAGGCCATGGCCC
TGCAGTCGATCCAAAACATGCGTGGGAATGCCCACTGTGTGGACTGTGAGACCCAGAATCCTAAGTGGGC
CAGTTTGAACTTGGGAGTCCTCATGTGTATTGAATGCTCAGGAATCCACCACAGTCTTGGCACCCGCCTT
TCCCGTGTGCGATCTCTGGAGCTGGATGACTGGCCAGTTGAGCTCAGGAAGGTTATGTCATCTATTGGCA
ATGACCTAGCCAACAGCATCTGGGAAGGGAGCAGCCAGGGGCAGACAAAACCCTCAGAAAAGTCCACGAG
GGAAGAGAAGGAACGGTGGATCCGTTCCAAATATGAGGAGAAGCTCTTTCTGGCCCCACTACCCTGCACT
GAGCTGTCCCTGGGCCAGCAGCTGCTGTGGGCCACCGCTGATGAGGACCTGCAGACAGCCATCCTGCTGC
TGGCACATGGCTCCCGTGAGGAGGTGAACGAGACCTGTGGGGAGGGAGACGGCTGCACGGCGCTCCATCT
GGCCTGCCGCAAGGGGAATGTGGTCCTGGCGCAGCTCCTGATCTGGTACGGGGTGGACGTCATGGCCCGA
GATGCCCACGGGAACACAGCGCTGACCTACGCCCGGCAGGCCTCCAGCCAGGAGTGCATCAACGTGCTTC
TGCAGTACGGCTGCCCCGACGAGTGTGTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC214807 representing NM_001077686
Red=Cloning site Green=Tags(s)

MGNILTCRVHPSVSLEFDQQQGSVCPSESEIYEAGAGDRMAGAPMAAAVQPAEVTVEVGEDLHMHHVRDR
EMPEALEFNPSANPEASTIFQRNSQTDVVEIRRSNCTNHVSTVRFSQQYSLCSTIFLDDSTAIQHYLTMT
IISVTLEIPHHITQRDADRSLSIPDEQLHSFAVSTVHIMKKRNGGGSLNNYSSSIPSTPSTSQEDPQFSV
PPTANTPTPVCKRSMRWSNLFTSEKGSDPDKERKAPENHADTIGSGRAIPIKQGMLLKRSGKWLKTWKKK
YVTLCSNGVLTYYSSLGDYMKNIHKKEIDLQTSTIKVPGKWPSLATSACTPISSSKSNGLSKDMDTGLGD
SICFSPSISSTTSPKLNPPPSPHANKKKHLKKKSTNNFMIVSATGQTWHFEATTYEERDAWVQAIQSQIL
ASLQSCESSKSKSQLTSQSEAMALQSIQNMRGNAHCVDCETQNPKWASLNLGVLMCIECSGIHHSLGTRL
SRVRSLELDDWPVELRKVMSSIGNDLANSIWEGSSQGQTKPSEKSTREEKERWIRSKYEEKLFLAPLPCT
ELSLGQQLLWATADEDLQTAILLLAHGSREEVNETCGEGDGCTALHLACRKGNVVLAQLLIWYGVDVMAR
DAHGNTALTYARQASSQECINVLLQYGCPDECV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001077686
ORF Size 1989 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001077686.2, NP_001071154.1
RefSeq Size 2420 bp
RefSeq ORF 1991 bp
Locus ID 728404
Cytogenetics 10q11.23
MW 72.9 kDa
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Documents
Resources
"NM_001077686" in other vectors (4)
SKU Description Size Price
RC214807L3 Lenti ORF clone of Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 8 (AGAP8), Myc-DDK-tagged 10 ug
$917.00
RC214807L4 Lenti ORF clone of Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 8 (AGAP8), mGFP tagged 10 ug
$917.00
RG214807 AGAP8 (tGFP-tagged) - Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 8 (AGAP8) 10 ug
$817.00
SC315606 AGAP8 (untagged)-Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 8 (AGAP8) 10 ug
$680.00

Plasmid Map for RC214807

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC214807
Circular map for RC214807

file-alt Citations

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