CENATAC (NM_198489) Human Tagged ORF Clone

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SKU
RC214707
CCDC84 (Myc-DDK-tagged)-Human coiled-coil domain containing 84 (CCDC84)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CENATAC
Synonyms CCDC84; DLNB14
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214707 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCCGGCGCAGCGCTGCCCTCTGTGCCGCCAGACCTTCTTCTGTGGTCGCGGGCACGTTTACAGCC
GCAAGCACCAGCGGCAGCTGAAGGAGGCTTTGGAGAGGCTCCTGCCCCAGGTGGAGGCGGCCCGCAAGGC
CATCCGCGCCGCTCAGGTGGAGCGCTATGTGCCCGAACACGAGCGATGCTGCTGGTGCCTGTGCTGCGGC
TGTGAGGTGCGGGAACACCTGAGCCATGGAAACCTGACGGTGCTGTACGGGGGGCTGCTGGAGCATCTGG
CCAGCCCAGAGCACAAGAAAGCAACCAACAAATTCTGGTGGGAGAACAAAGCTGAGGTCCAGATGAAAGA
GAAGTTTCTGGTCACTCCCCAGGATTATGCGCGATTCAAGAAATCCATGGTGAAAGGTTTGGATTCCTAT
GAAGAAAAGGAGGATAAAGTGATCAAGGAGATGGCAGCTCAGATCCGTGAGGTGGAGCAGAGCCGACAGG
AGGTGGTTCGGTCTGTCTTAGAGCCTCAGGCAGTGCCAGACCCAGAAGAGGGCTCTTCAGCACCTAGAAG
CTGGAAAGGGATGAACAGCCAAGTAGCTTCCAGCTTACAGCAGCCCTCAAATTTGGACCTGCCACCAGCT
CCAGAGCTTGACTGGATGGAGACAGGACCATCTCTGACATTCATTGGCCATCAGGATATACCAGGAGTTG
GTAACATCCACTCAGGTGCCACACCTCCCTGGATGATCCAAGATGAAGAATACATTGCTGGGAACCAAGA
AATAGGACCATCCTATGAAGAATTTCTTAAAGAAAAGGAAAAACAGAAGTTGAAAAAACTCCCCCCAGAC
CGAGTTGGGGCCAACTTTGATCACAGCTCCAGGACCAGTGCAGGCTGGCTGCCCTCTTTTGGCCGCGTCT
GGAATAATGGACGCCGCTGGCAGTCCAGACATCAATTCAAAACTGAAGCTGCAGCAATGAAGAAGCAGTC
ACATACAGAAAAAAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC214707 protein sequence
Red=Cloning site Green=Tags(s)

MAPAQRCPLCRQTFFCGRGHVYSRKHQRQLKEALERLLPQVEAARKAIRAAQVERYVPEHERCCWCLCCG
CEVREHLSHGNLTVLYGGLLEHLASPEHKKATNKFWWENKAEVQMKEKFLVTPQDYARFKKSMVKGLDSY
EEKEDKVIKEMAAQIREVEQSRQEVVRSVLEPQAVPDPEEGSSAPRSWKGMNSQVASSLQQPSNLDLPPA
PELDWMETGPSLTFIGHQDIPGVGNIHSGATPPWMIQDEEYIAGNQEIGPSYEEFLKEKEKQKLKKLPPD
RVGANFDHSSRTSAGWLPSFGRVWNNGRRWQSRHQFKTEAAAMKKQSHTEKS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_198489
ORF Size 996 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_198489.3
RefSeq Size 1274 bp
RefSeq ORF 999 bp
Locus ID 338657
UniProt ID Q86UT8
Cytogenetics 11q23.3
MW 38 kDa
Summary This gene encodes a protein thought to contain a coiled coil motif. No function has been determined for the encoded protein. A pseudogene of this gene is located on chromosome 20. Alternate splicing results in multiple transcript variants. provided by RefSeq, Aug 2013
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Documents
Resources
"NM_198489" in other vectors (4)
SKU Description Size Price
RC214707L3 Lenti ORF clone of Human coiled-coil domain containing 84 (CCDC84), Myc-DDK-tagged 10 ug
$600.00
RC214707L4 Lenti ORF clone of Human coiled-coil domain containing 84 (CCDC84), mGFP tagged 10 ug
$600.00
RG214707 CCDC84 (tGFP-tagged) - Human coiled-coil domain containing 84 (CCDC84) 10 ug
$489.00 $500.00
SC309378 CCDC84 (untagged)-Human coiled-coil domain containing 84 (CCDC84) 10 ug
$330.00

Plasmid Map for RC214707

Download sequence for

Cloning scheme for RC214707

Circular map for RC214707

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