BVES (NM_147147) Human Tagged ORF Clone

  • TrueORF®
SKU
RC214565
BVES (Myc-DDK-tagged)-Human blood vessel epicardial substance (BVES), transcript variant B
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol BVES
Synonyms CARICK; HBVES; LGMD2X; LGMDR25; POP1; POPDC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214565 representing NM_147147
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATTATACAGAGTCCAGCCCATTGAGAGAATCAACTGCCATAGGTTTTACACCTGAGTTAGAAAGTA
TCATACCTGTGCCTTCCAATAAGACCACTTGTGAAAACTGGAGAGAGATACATCATCTGGTTTTTCATGT
AGCAAATATTTGTTTTGCAGTTGGGTTGGTTATTCCAACTACTCTTCACCTTCATATGATATTTCTTAGG
GGAATGTTAACTCTAGGATGTACCCTTTATATCGTCTGGGCCACTCTCTACCGATGTGCCTTGGATATAA
TGATCTGGAACTCTGTGTTCTTGGGTGTCAACATTTTGCATCTGTCGTATCTTTTATACAAGAAGAGACC
GGTAAAGATTGAAAAGGAACTCAGTGGCATGTACCGGCGATTGTTTGAACCACTCCGTGTGCCTCCAGAT
TTGTTCAGAAGACTAACTGGACAGTTTTGCATGATCCAAACCTTGAAAAAGGGCCAAACTTATGCTGCAG
AGGATAAAACCTCAGTTGATGACCGTCTGAGTATTCTCTTGAAGGGAAAAATGAAGGTCTCCTATCGAGG
ACATTTTCTGCATAACATTTACCCCTGTGCCTTTATAGATTCTCCTGAATTTAGATCAACTCAGATGCAC
AAAGGTGAAAAATTCCAGGTCACCATTATTGCAGATGATAACTGCAGATTTTTATGCTGGTCAAGAGAAA
GATTAACATACTTTCTGGAATCAGAACCTTTCTTGTATGAAATCTTTAGGTATCTTATTGGAAAAGACAT
CACAAATAAGCTCTACTCATTGAATGATCCCACCTTAAATGATAAAAAAGCCAAAAAGCTGGAACATCAG
CTCAGCCTCTGCACACAGATCTCCATGTTGGAAATGAGGAACAGTATAGCCAGCTCCAGTGACAGTGACG
ACGGCTTGCACCAGTTTCTTCGGGGTACCTCCAGCATGTCCTCTCTTCATGTGTCATCCCCACACCAGCG
AGCCTCTGCCAAGATGAAACCGATAGAAGAAGGAGCAGAAGATGATGATGACGTTTTTGAACCGGCATCT
CCAAATACATTGAAAGTCCATCAGCTGCCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC214565 representing NM_147147
Red=Cloning site Green=Tags(s)

MNYTESSPLRESTAIGFTPELESIIPVPSNKTTCENWREIHHLVFHVANICFAVGLVIPTTLHLHMIFLR
GMLTLGCTLYIVWATLYRCALDIMIWNSVFLGVNILHLSYLLYKKRPVKIEKELSGMYRRLFEPLRVPPD
LFRRLTGQFCMIQTLKKGQTYAAEDKTSVDDRLSILLKGKMKVSYRGHFLHNIYPCAFIDSPEFRSTQMH
KGEKFQVTIIADDNCRFLCWSRERLTYFLESEPFLYEIFRYLIGKDITNKLYSLNDPTLNDKKAKKLEHQ
LSLCTQISMLEMRNSIASSSDSDDGLHQFLRGTSSMSSLHVSSPHQRASAKMKPIEEGAEDDDDVFEPAS
PNTLKVHQLP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_147147
ORF Size 1080 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_147147.3
RefSeq Size 5507 bp
RefSeq ORF 1083 bp
Locus ID 11149
UniProt ID Q8NE79
Cytogenetics 6q21
Protein Families Transmembrane
MW 41.3 kDa
Summary This gene encodes a member of the POP family of proteins containing three putative transmembrane domains. This gene is expressed in cardiac and skeletal muscle and may play an important role in development of these tissues. The mouse ortholog may be involved in the regeneration of adult skeletal muscle and may act as a cell adhesion molecule in coronary vasculogenesis. Three transcript variants encoding the same protein have been found for this gene. provided by RefSeq, Dec 2010
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Documents
Resources
"NM_147147" in other vectors (4)
SKU Description Size Price
RC214565L3 Lenti ORF clone of Human blood vessel epicardial substance (BVES), transcript variant B, Myc-DDK-tagged 10 ug
$757.00
RC214565L4 Lenti ORF clone of Human blood vessel epicardial substance (BVES), transcript variant B, mGFP tagged 10 ug
$757.00
RG214565 BVES (tGFP-tagged) - Human blood vessel epicardial substance (BVES), transcript variant B 10 ug
$657.00
SC125867 BVES (untagged)-Human blood vessel epicardial substance (BVES), transcript variant B 10 ug
$457.00

Plasmid Map for RC214565

Download sequence for

Cloning scheme for RC214565

Circular map for RC214565

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