C2GNT3 (GCNT4) (NM_016591) Human Tagged ORF Clone

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SKU
RC214560
GCNT4 (Myc-DDK-tagged)-Human glucosaminyl (N-acetyl) transferase 4, core 2 (GCNT4)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol C2GNT3
Synonyms C2GNT3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214560 representing NM_016591
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGATATTCAAATGTTATTTTAAACATACCCTACAGCAGAAAGTTTTCATCCTGTTTTTAACCCTAT
GGCTGCTCTCTTTGTTAAAGCTTCTAAATGTGAGACGACTCTTTCCGCAAAAAGACATTTACTTGGTTGA
GTACTCCCTAAGTACCTCGCCTTTTGTAAGAAACAGATACACTCATGTTAAGGATGAAGTCAGGTATGAA
GTTAACTGTTCGGGTATCTATGAACAGGAGCCTTTGGAAATTGGAAAGAGTCTGGAAATAAGAAGAAGGG
ACATCATTGACTTGGAGGATGATGATGTTGTGGCAATGACCAGTGATTGTGACATTTATCAGACTCTAAG
AGGTTATGCTCAAAAGCTTGTCTCAAAGGAGGAGAAAAGCTTCCCAATAGCCTATTCTTTGGTTGTCCAC
AAAGATGCAATTATGGTTGAAAGGCTTATCCATGCTATATACAACCAGCACAATATTTACTGCATCCATT
ATGATCGTAAGGCACCTGATACCTTCAAAGTTGCCATGAACAATTTAGCTAAGTGCTTCTCCAATATTTT
CATTGCTTCCAAATTAGAGGCTGTGGAATATGCCCACATTTCCAGACTCCAGGCTGATTTAAATTGCTTG
TCGGACCTTCTGAAGTCTTCAATCCAGTGGAAATATGTTATCAACTTGTGTGGGCAAGATTTTCCCCTGA
AGTCAAATTTTGAATTGGTGTCAGAGTTGAAAAAACTCAATGGAGCAAATATGTTGGAGACGGTGAAACC
CCCAAACAGTAAATTGGAAAGATTCACTTACCATCATGAACTTAGACGGGTGCCTTATGAATATGTGAAG
CTACCAATAAGGACAAACATCTCCAAGGAAGCACCCCCCCATAACATTCAGATATTTGTTGGCAGTGCTT
ATTTTGTTTTAAGTCAAGCATTTGTTAAATATATTTTCAACAACTCCATCGTTCAAGACTTTTTTGCCTG
GTCTAAAGACACATACTCTCCTGATGAGCACTTTTGGGCTACCTTGATTCGGGTTCCAGGAATACCTGGG
GAGATTTCCAGATCAGCCCAGGATGTGTCTGATCTGCAGAGTAAGACTCGCCTTGTCAAGTGGAATTACT
ATGAAGGCTTTTTCTATCCCAGTTGTACTGGATCTCACCTTCGAAGCGTGTGTATTTATGGAGCTGCAGA
ATTAAGGTGGCTTATCAAAGATGGACATTGGTTTGCTAATAAATTTGATTCTAAGGTGGACCCTATCTTG
ATTAAATGCTTGGCAGAAAAGCTTGAAGAACAGCAGAGAGACTGGATCACTTTGCCCTCAGAAAAGTTAT
TTATGGATAGAAATCTCACTACCACATCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC214560 representing NM_016591
Red=Cloning site Green=Tags(s)

MKIFKCYFKHTLQQKVFILFLTLWLLSLLKLLNVRRLFPQKDIYLVEYSLSTSPFVRNRYTHVKDEVRYE
VNCSGIYEQEPLEIGKSLEIRRRDIIDLEDDDVVAMTSDCDIYQTLRGYAQKLVSKEEKSFPIAYSLVVH
KDAIMVERLIHAIYNQHNIYCIHYDRKAPDTFKVAMNNLAKCFSNIFIASKLEAVEYAHISRLQADLNCL
SDLLKSSIQWKYVINLCGQDFPLKSNFELVSELKKLNGANMLETVKPPNSKLERFTYHHELRRVPYEYVK
LPIRTNISKEAPPHNIQIFVGSAYFVLSQAFVKYIFNNSIVQDFFAWSKDTYSPDEHFWATLIRVPGIPG
EISRSAQDVSDLQSKTRLVKWNYYEGFFYPSCTGSHLRSVCIYGAAELRWLIKDGHWFANKFDSKVDPIL
IKCLAEKLEEQQRDWITLPSEKLFMDRNLTTTS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016591
ORF Size 1359 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016591.2, NP_057675.1
RefSeq Size 3435 bp
RefSeq ORF 1362 bp
Locus ID 51301
Cytogenetics 5q13.3
Protein Families Transmembrane
Protein Pathways Metabolic pathways, O-Glycan biosynthesis
MW 52.9 kDa
Summary Glycosyltransferase that mediates core 2 O-glycan branching, an important step in mucin-type biosynthesis. Does not have core 4 O-glycan or I-branching enzyme activity.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_016591" in other vectors (4)
SKU Description Size Price
RC214560L3 Lenti ORF clone of Human glucosaminyl (N-acetyl) transferase 4, core 2 (GCNT4), Myc-DDK-tagged 10 ug
$757.00
RC214560L4 Lenti ORF clone of Human glucosaminyl (N-acetyl) transferase 4, core 2 (GCNT4), mGFP tagged 10 ug
$757.00
RG214560 GCNT4 (tGFP-tagged) - Human glucosaminyl (N-acetyl) transferase 4, core 2 (GCNT4) 10 ug
$657.00
SC304436 GCNT4 (untagged)-Human glucosaminyl (N-acetyl) transferase 4, core 2 (GCNT4) 10 ug
$503.00

Plasmid Map for RC214560

Download sequence for

Cloning scheme for RC214560

Circular map for RC214560

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