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DNAJC4 (NM_005528) Human Tagged ORF Clone

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SKU
RC214506
DNAJC4 (Myc-DDK-tagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 4 (DNAJC4)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DNAJC4
Synonyms DANJC4; HSPF2; MCG18
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214506 representing NM_005528
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGCCCTTACTGCCCCTGCGCCTGTGCCGGCTGTGGCCCCGCAACCCTCCCTCCCGGCTCCTCGGAG
CGGCCGCCGGGCAGCGGTCCAGACCCAGTACTTATTATGAACTGTTGGGGGTGCATCCTGGTGCCAGCAC
TGAGGAAGTTAAACGAGCTTTCTTCTCCAAGTCCAAAGAGCTGCACCCAGACCGGGACCCTGGGAACCCA
AGCCTGCACAGCCGCTTTGTGGAGCTGAGCGAGGCATACCGTGTGCTCAGCCGTGAGCAGAGCCGCCGCA
GCTATGATGACCAGCTCCGCTCAGGTAGTCCCCCAAAGTCTCCACGAACCACAGTCCATGACAAGTCTGC
CCACCAAACACACAGCTCCTGGACACCCCCCAACGCACAGTACTGGTCCCAGTTTCACAGCGTGAGGCCA
CAGGGGCCCCAGTTGAGGCAGCAGCAACACAAACAAAACAAACAAGTGCTGGGGTACTGCCTCCTCCTCA
TGCTGGCGGGCATGGGCCTGCACTACATTGCCTTCAGGAAGGTGAAGCAGATGCACCTTAACTTCATGGA
TGAAAAGGATCGGATCATCACAGCCTTCTACAACGAAGCCCGGGCACGGGCCAGGGCCAACAGAGGCATC
CTTCAGCAGGAGCGACAACGGCTAGGGCAGCGGCAGCCGCCACCATCCGAGCCAACCCAAGGCCCCGAGA
TCGTGCCCCGGGGCGCCGGCCCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC214506 representing NM_005528
Red=Cloning site Green=Tags(s)

MPPLLPLRLCRLWPRNPPSRLLGAAAGQRSRPSTYYELLGVHPGASTEEVKRAFFSKSKELHPDRDPGNP
SLHSRFVELSEAYRVLSREQSRRSYDDQLRSGSPPKSPRTTVHDKSAHQTHSSWTPPNAQYWSQFHSVRP
QGPQLRQQQHKQNKQVLGYCLLLMLAGMGLHYIAFRKVKQMHLNFMDEKDRIITAFYNEARARARANRGI
LQQERQRLGQRQPPPSEPTQGPEIVPRGAGP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005528
ORF Size 723 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005528.3, NP_005519.2
RefSeq Size 1214 bp
RefSeq ORF 726 bp
Locus ID 3338
UniProt ID Q9NNZ3
Cytogenetics 11q13.1
Protein Families Transmembrane
MW 27.4 kDa
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Documents
Resources
"NM_005528" in other vectors (4)
SKU Description Size Price
RC214506L3 Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily C, member 4 (DNAJC4), Myc-DDK-tagged 10 ug
$600.00
RC214506L4 Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily C, member 4 (DNAJC4), mGFP tagged 10 ug
$600.00
RG214506 DNAJC4 (tGFP-tagged) - Human DnaJ (Hsp40) homolog, subfamily C, member 4 (DNAJC4) 10 ug
$500.00
SC317345 DNAJC4 (untagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 4 (DNAJC4) 10 ug
$330.00

Plasmid Map for RC214506

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC214506
Circular map for RC214506

file-alt Citations

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